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Generation of a Bacterium with a 21 Amino Acid Genetic Code
Abstract
We have generated a completely autonomous bacterium with a 21 amino acid genetic code. This bacterium can biosynthesize a nonstandard amino acid from basic carbon sources and incorporate this amino acid into proteins in response to the amber nonsense codon. The biosynthetic pathway for the amino acid p-aminophenylalanine (pAF) as well as a unique pAF synthetase and cognate tRNA were added to Escherichia coli. Denaturing gel electrophoresis and mass spectrometric analysis show that pAF is incorporated into myoglobin with fidelity and efficiency rivaling those of the common 20 amino acids. This and other such organisms may provide an opportunity to examine the evolutionary consequences of adding new amino acids to the genetic repertoire, as well as generate proteins with new or enhanced biological functions.
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Cites
Osawa, S. (1995) · doi:10.1093/oso/9780198547815.001.0001 · Cited by 216
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Adding New Chemistries to the Genetic Code
Liu, C. C., & Schultz, P. G. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.052308.105824 · Cited by 1,725
Self-citationCellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins
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Click Chemistry in Complex Mixtures: Bioorthogonal Bioconjugation
McKay, C. S., & Finn, M. G. (2014) · Chemistry & Biology · doi:10.1016/j.chembiol.2014.09.002 · Cited by 768
Bioorthogonale Chemie – oder: in einem Meer aus Funktionalität nach Selektivität fischen
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Metadata
- Authors
- Mehl, R. A., Anderson, J. C., Santoro, S. W., Wang, L., Martin, A. B., King, D. S., Horn, D. M., & Schultz, P. G.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-01-01
- Volume
- 125
- Issue
- 4
- Pages
- 935-939
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2017602203
- Cited by
- 287
- References
- 1
- FWCI
- 5.68
- Type
- journal article
- Language
- en
- Open access
- closed
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- Mehl, R. A., Anderson, J. C., Santoro, S. W., Wang, L., Martin, A. B., King, D. S., Horn, D. M., & Schultz, P. G. (2003). Generation of a Bacterium with a 21 Amino Acid Genetic Code. Journal of the American Chemical Society. https://doi.org/10.1021/ja0284153
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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