The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Screening of Protein Crystallization Conditions on a Microfluidic Chip Using Nanoliter-Size Droplets
Abstract
Protein crystallization is a major bottleneck in determining tertiary protein structures from genomic sequence data. This paper describes a microfluidic system for screening hundreds of protein crystallization conditions using less than 4 nL of protein solution for each crystallization droplet. The droplets are formed by mixing protein, precipitant, and additive stock solutions in variable ratios in a flow of water-immiscible fluids inside microchannels. Each droplet represents a discrete trial testing different conditions. The system has been validated by crystallization of several water-soluble proteins.
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Citations
Cites
Vesicles and Polymerized Vesicles from Thiophene‐Containing Rod–Coil Block Copolymers
Vriezema, D. M., Hoogboom, J., Velonia, K., Takazawa, K., Christianen, P. C. M., Maan, J. C., Rowan, A. E., & Nolte, R. J. M. (2003) · Angewandte Chemie International Edition · doi:10.1002/anie.200390204 · Cited by 334
Cited by
Teh, S.-Y., Lin, R., Hung, L.-H., & Lee, A. P. (2008) · Lab on a Chip · doi:10.1039/b715524g · Cited by 2,690
Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications
Mark, D., Haeberle, S., Roth, G., von Stetten, F., & Zengerle, R. (2010) · Chemical Society Reviews · doi:10.1039/b820557b · Cited by 1,647
Whitesides, G. M. (2018) · Angewandte Chemie International Edition · doi:10.1002/anie.201800907 · Cited by 850
Synthesis of micro and nanostructures in microfluidic systems
Marre, S., & Jensen, K. F. (2010) · Chemical Society Reviews · doi:10.1039/b821324k · Cited by 734
Microfluidic cell culture systems for drug research
Wu, M.-H., Huang, S.-B., & Lee, G.-B. (2010) · Lab on a Chip · doi:10.1039/b921695b · Cited by 432
Girod, M., Moyano, E., Campbell, D. I., & Cooks, R. G. (2011) · Chem. Sci. · doi:10.1039/c0sc00416b · Cited by 419
Zheng, B., Tice, J. D., Roach, L. S., & Ismagilov, R. F. (2004) · Angewandte Chemie International Edition · doi:10.1002/anie.200453974 · Cited by 398
Self-citationDroplet Control for Microfluidics
Joanicot, M., & Ajdari, A. (2005) · Science · doi:10.1126/science.1112615 · Cited by 379
Monodispersed microfluidic droplet generation by shear focusing microfluidic device
Tan, Y.-C., Cristini, V., & Lee, A. P. (2006) · Sensors and Actuators B: Chemical · doi:10.1016/j.snb.2005.06.008 · Cited by 305
Permeation-driven flow in poly(dimethylsiloxane) microfluidic devices.
Randall, G. C., & Doyle, P. S. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0503287102 · Cited by 220
Chip in a lab: microfluidics for next generation life science research
Streets, A. M., & Huang, Y. (2013) · Biomicrofluidics · doi:10.1063/1.4789751 · Cited by 180
Metadata
- Authors
- Zheng, B., Roach, L. S., & Ismagilov, R. F.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-08-23
- Volume
- 125
- Issue
- 37
- Pages
- 11170-11171
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2020068704
- Cited by
- 697
- References
- 1
- FWCI
- 22.29
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
