The DOI matches its Crossref record.
Mesoporous Silica Nanoparticles for Intracellular Delivery of Membrane-Impermeable Proteins
Abstract
An MCM-41-type mesoporous silica nanoparticle (MSN) material with a large average pore diameter (5.4 nm) is synthesized and characterized. The in vitro uptake and release profiles of cytochrome c by the MSN were investigated. The enzymatic activity of the released protein was quantitatively analyzed and compared with that of the native cytochrome c in physiological buffer solutions. We found that the enzymes released from the MSNs are still functional and highly active in catalyzing the oxidation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) by hydrogen peroxide. In contrast to the fact that cytochrome c is a cell-membrane-impermeable protein, we discovered that the cytochrome c-encapsulated MSNs could be internalized by live human cervical cancer cells (HeLa) and the protein could be released into the cytoplasm. We envision that these MSNs with large pores could serve as a transmembrane delivery vehicle for controlled release of membrane-impermeable proteins in live cells, which may lead to many important biotechnological applications including therapeutics and metabolic manipulation of cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Applications of Nanoparticles in Biology
De, M., Ghosh, P. S., & Rotello, V. M. (2008) · Advanced Materials · doi:10.1002/adma.200703183 · Cited by 1,547
Mesoporous silica nanoparticles in drug delivery and biomedical applications
Wang, Y., Zhao, Q., Han, N., Bai, L., Li, J., Liu, J., Che, E., Hu, L., Zhang, Q., Jiang, T., et al. (2015) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2014.09.014 · Cited by 1,192
Chen, Z., Chen, H., Hu, H., Yu, M., Li, F., Zhang, Q., Zhou, Z., Yi, T., & Huang, C. (2008) · Journal of the American Chemical Society · doi:10.1021/ja076151k · Cited by 836
Shieh, F.-K., Wang, S.-C., Yen, C.-I., Wu, C.-C., Dutta, S., Chou, L.-Y., Morabito, J. V., Hu, P., Hsu, M.-H., Wu, K. C.-W., et al. (2015) · Journal of the American Chemical Society · doi:10.1021/ja513058h · Cited by 774
Croissant, J. G., Fatieiev, Y., Almalik, A., & Khashab, N. M. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201700831 · Cited by 594
Metadata
- Authors
- Slowing, I. I., Trewyn, B. G., & Lin, V. S.-Y.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-06-23
- Volume
- 129
- Issue
- 28
- Pages
- 8845-8849
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2073341588
- Cited by
- 795
- References
- 0
- FWCI
- 28.34
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Mesoporous Silica Nanoparticles for Intracellular Delivery of Membrane-Impermeable Proteins. https://app.cochat.ai/papers/10.1021/ja0719780
- The paper
- Slowing, I. I., Trewyn, B. G., & Lin, V. S.-Y. (2007). Mesoporous Silica Nanoparticles for Intracellular Delivery of Membrane-Impermeable Proteins. Journal of the American Chemical Society. https://doi.org/10.1021/ja0719780
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
