The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Preparation of Fe3O4@SiO2@Layered Double Hydroxide Core–Shell Microspheres for Magnetic Separation of Proteins
Abstract
Three-component microspheres containing an SiO(2)-coated Fe(3)O(4) magnetite core and a layered double hydroxide (LDH) nanoplatelet shell have been synthesized via an in situ growth method. The resulting Fe(3)O(4)@SiO(2)@NiAl-LDH microspheres display three-dimensional core-shell architecture with flowerlike morphology, large surface area (83 m(2)/g), and uniform mesochannels (4.3 nm). The Ni(2+) cations in the NiAl-LDH shell provide docking sites for histidine and the materials exhibit excellent performance in the separation of a histidine (His)-tagged green fluorescent protein, with a binding capacity as high as 239 μg/mg. The microspheres show highly selective adsorption of the His-tagged protein from Escherichia coli lysate, demonstrating their practical applicability. Moreover, the microspheres possess superparamagnetism and high saturation magnetization (36.8 emu/g), which allows them to be easily separated from solution by means of an external magnetic field and subsequently reused. The high stability and selectivity of the Fe(3)O(4)@SiO(2)@NiAl-LDH microspheres for the His-tagged protein were retained over several separation cycles. Therefore, this work provides a promising approach for the design and synthesis of multifunctional LDH microspheres, which can be used for the practical purification of recombinant proteins, as well as having other potential applications in a variety of biomedical fields including drug delivery and biosensors.
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Cited by
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Metadata
- Authors
- Shao, M., Ning, F., Zhao, J., Wei, M., Evans, D. G., & Duan, X.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-01-06
- Volume
- 134
- Issue
- 2
- Pages
- 1071-1077
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2073732456
- Cited by
- 803
- References
- 22
- FWCI
- 23.08
- Type
- journal article
- Language
- en
- Open access
- closed
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- Shao, M., Ning, F., Zhao, J., Wei, M., Evans, D. G., & Duan, X. (2012). Preparation of Fe3O4@SiO2@Layered Double Hydroxide Core–Shell Microspheres for Magnetic Separation of Proteins. Journal of the American Chemical Society. https://doi.org/10.1021/ja2086323
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