The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification
Abstract
Through rational design of a functional molecular probe with high sequence specificity that takes advantage of sensitive isothermal amplification with simple operation, we developed a one-pot hairpin-mediated quadratic enzymatic amplification strategy for microRNA (miRNA) detection. Our method exhibits ultrahigh sensitivity toward miR-21 with detection limits of 10 fM at 37 °C and 1 aM at 4 °C, which corresponds to nine strands of miR-21 in a 15 μL sample, and it is capable of distinguishing among miRNA family members. More importantly, the proposed approach is also sensitive and selective when applied to crude extractions from MCF-7 and PC3 cell lines and even patient tissues from intraductal carcinoma and invasive ductal carcinoma of the breast.
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Cites
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
Lee, R. C., Feinbaum, R. L., & Ambros, V. (1993) · Cell · doi:10.1016/0092-8674(93)90529-y · Cited by 12,948
Identification of Novel Genes Coding for Small Expressed RNAs
Lagos-Quintana, M., Rauhut, R., Lendeckel, W., & Tuschl, T. (2001) · Science · doi:10.1126/science.1064921 · Cited by 4,956
Cited by
Noncanonical Self-Assembly of Multifunctional DNA Nanoflowers for Biomedical Applications
Zhu, G., Hu, R., Zhao, Z., Chen, Z., Zhang, X., & Tan, W. (2013) · Journal of the American Chemical Society · doi:10.1021/ja406115e · Cited by 429
Self-citationDensity gradient ultracentrifugation for colloidal nanostructures separation and investigation
Li, P., Kumar, A., Ma, J., Kuang, Y., Luo, L., & Sun, X. (2018) · Science Bulletin · doi:10.1016/j.scib.2018.04.014 · Cited by 67
Metadata
- Authors
- Duan, R., Zuo, X., Wang, S., Quan, X., Chen, D., Chen, Z., Jiang, L., Fan, C., & Xia, F.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-03-13
- Volume
- 135
- Issue
- 12
- Pages
- 4604-4607
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2078653411
- Cited by
- 355
- References
- 2
- FWCI
- 17.75
- Type
- journal article
- Language
- en
- Open access
- closed
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- Duan, R., Zuo, X., Wang, S., Quan, X., Chen, D., Chen, Z., Jiang, L., Fan, C., & Xia, F. (2013). Lab in a Tube: Ultrasensitive Detection of MicroRNAs at the Single-Cell Level and in Breast Cancer Patients Using Quadratic Isothermal Amplification. Journal of the American Chemical Society. https://doi.org/10.1021/ja311313b
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