The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
The Transcriptional Complex Between the BCL2 i-Motif and hnRNP LL Is a Molecular Switch for Control of Gene Expression That Can Be Modulated by Small Molecules
Open accessAbstract
In a companion paper (DOI: 10.021/ja410934b) we demonstrate that the C-rich strand of the cis-regulatory element in the BCL2 promoter element is highly dynamic in nature and can form either an i-motif or a flexible hairpin. Under physiological conditions these two secondary DNA structures are found in an equilibrium mixture, which can be shifted by the addition of small molecules that trap out either the i-motif (IMC-48) or the flexible hairpin (IMC-76). In cellular experiments we demonstrate that the addition of these molecules has opposite effects on BCL2 gene expression and furthermore that these effects are antagonistic. In this contribution we have identified a transcriptional factor that recognizes and binds to the BCL2 i-motif to activate transcription. The molecular basis for the recognition of the i-motif by hnRNP LL is determined, and we demonstrate that the protein unfolds the i-motif structure to form a stable single-stranded complex. In subsequent experiments we show that IMC-48 and IMC-76 have opposite, antagonistic effects on the formation of the hnRNP LL-i-motif complex as well as on the transcription factor occupancy at the BCL2 promoter. For the first time we propose that the i-motif acts as a molecular switch that controls gene expression and that small molecules that target the dynamic equilibrium of the i-motif and the flexible hairpin can differentially modulate gene expression.
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Cites
Quantitative visualization of DNA G-quadruplex structures in human cells
Biffi, G., Tannahill, D., McCafferty, J., & Balasubramanian, S. (2013) · Nature Chemistry · doi:10.1038/nchem.1548 · Cited by 2,146
Dai, J., Dexheimer, T. S., Chen, D., Carver, M., Ambrus, A., Jones, R. A., & Yang, D. (2006) · Journal of the American Chemical Society · doi:10.1021/ja055636a · Cited by 413
Sun, D., & Hurley, L. H. (2009) · Journal of Medicinal Chemistry · doi:10.1021/jm900055s · Cited by 397
Self-citationHeterogeneous Nuclear Ribonucleoprotein K Is a Transcription Factor
Michelotti, E. F., Michelotti, G. A., Aronsohn, A. I., & Levens, D. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.5.2350 · Cited by 353
Functional complexity and regulation through RNA dynamics
Dethoff, E. A., Chugh, J., Mustoe, A. M., & Al-Hashimi, H. M. (2012) · Nature · doi:10.1038/nature10885 · Cited by 328
Identification and Characterization of Nucleolin as a c-myc G-quadruplex-binding Protein
González, V., Guo, K., Hurley, L., & Sun, D. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.018028 · Cited by 321
Self-citationBrooks, T. A., & Hurley, L. H. (2009) · Nature Reviews Cancer · doi:10.1038/nrc2733 · Cited by 294
Self-citationKendrick, S., Kang, H.-J., Alam, M. P., Madathil, M. M., Agrawal, P., Gokhale, V., Yang, D., Hecht, S. M., & Hurley, L. H. (2014) · Journal of the American Chemical Society · doi:10.1021/ja410934b · Cited by 281
Self-citationA stress-responsive RNA switch regulates VEGFA expression
Ray, P. S., Jia, J., Yao, P., Majumder, M., Hatzoglou, M., & Fox, P. L. (2009) · Nature · doi:10.1038/nature07598 · Cited by 249
Rajendran, A., Nakano, S.-I., & Sugimoto, N. (2010) · Chemical Communications · doi:10.1039/b922050j · Cited by 210
Cited by
The Structure and Function of DNA G-Quadruplexes
Spiegel, J., Adhikari, S., & Balasubramanian, S. (2020) · Trends in Chemistry · doi:10.1016/j.trechm.2019.07.002 · Cited by 920
“rich opposite strand may be single stranded and potentially complexed with single-stranded binding proteins [110,111].”
Backgroundi-Motif DNA: structural features and significance to cell biology
Abou Assi, H., Garavís, M., González, C., & Damha, M. J. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky735 · Cited by 423
i-Motif DNA: Structure, stability and targeting with ligands
Day, H. A., Pavlou, P., & Waller, Z. A. E. (2014) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2014.05.047 · Cited by 383
G-quadruplexes are transcription factor binding hubs in human chromatin
Spiegel, J., Cuesta, S. M., Adhikari, S., Hänsel-Hertsch, R., Tannahill, D., & Balasubramanian, S. (2021) · Genome Biology · doi:10.1186/s13059-021-02324-z · Cited by 267
Interplay of Guanine Oxidation and G-Quadruplex Folding in Gene Promoters
Fleming, A. M., & Burrows, C. J. (2020) · Journal of the American Chemical Society · doi:10.1021/jacs.9b11050 · Cited by 152
Metadata
- Authors
- Kang, H.-J., Kendrick, S., Hecht, S. M., & Hurley, L. H.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2014-03-07
- Volume
- 136
- Issue
- 11
- Pages
- 4172-4185
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W1972485143
- Cited by
- 257
- References
- 25
- FWCI
- 8.03
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Kang, H.-J., Kendrick, S., Hecht, S. M., & Hurley, L. H. (2014). The Transcriptional Complex Between the BCL2 i-Motif and hnRNP LL Is a Molecular Switch for Control of Gene Expression That Can Be Modulated by Small Molecules. Journal of the American Chemical Society. https://doi.org/10.1021/ja4109352
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