The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Direct In-Gel Fluorescence Detection and Cellular Imaging of O -GlcNAc-Modified Proteins
Open accessAbstract
We report an advanced chemoenzymatic strategy for the direct fluorescence detection, proteomic analysis, and cellular imaging of O-GlcNAc-modified proteins. O-GlcNAc residues are selectively labeled with fluorescent or biotin tags using an engineered galactosyltransferase enzyme and [3 + 2] azide-alkyne cycloaddition chemistry. We demonstrate that this approach can be used for direct in-gel detection and mass spectrometric identification of O-GlcNAc proteins, identifying 146 novel glycoproteins from the mammalian brain. Furthermore, we show that the method can be exploited to quantify dynamic changes in cellular O-GlcNAc levels and to image O-GlcNAc-glycosylated proteins within cells. As such, this strategy enables studies of O-GlcNAc glycosylation that were previously inaccessible and provides a new tool for uncovering the physiological functions of O-GlcNAc.
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Citations
Cites
Cycling of O-linked β-N-acetylglucosamine on nucleocytoplasmic proteins
Hart, G. W., Housley, M. P., & Slawson, C. (2007) · Nature · doi:10.1038/nature05815 · Cited by 1,418
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Phosphofructokinase 1 Glycosylation Regulates Cell Growth and Metabolism
Yi, W., Clark, P. M., Mason, D. E., Keenan, M. C., Hill, C., Goddard, W. A., Peters, E. C., Driggers, E. M., & Hsieh-Wilson, L. C. (2012) · Science · doi:10.1126/science.1222278 · Cited by 676
Self-citationAgarwal, P., & Bertozzi, C. R. (2015) · Bioconjugate Chemistry · doi:10.1021/bc5004982 · Cited by 605
Click Chemistry in Proteomic Investigations
Parker, C. G., & Pratt, M. R. (2020) · Cell · doi:10.1016/j.cell.2020.01.025 · Cited by 380
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Rao, X., Duan, X., Mao, W., Li, X., Li, Z., Li, Q., Zheng, Z., Xu, H., Chen, M., Wang, P. G., et al. (2015) · Nature Communications · doi:10.1038/ncomms9468 · Cited by 257
Transmembrane proteins tetraspanin 4 and CD9 sense membrane curvature
Dharan, R., Goren, S., Cheppali, S. K., Shendrik, P., Brand, G., Vaknin, A., Yu, L., Kozlov, M. M., & Sorkin, R. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2208993119 · Cited by 70
Metadata
- Authors
- Clark, P. M., Dweck, J. F., Mason, D. E., Hart, C. R., Buck, S. B., Peters, E. C., Agnew, B. J., & Hsieh-Wilson, L. C.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-08-07
- Volume
- 130
- Issue
- 35
- Pages
- 11576-11577
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2050554863
- Cited by
- 243
- References
- 1
- FWCI
- 3.72
- Type
- journal article
- Language
- en
- Open access
- green
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- Clark, P. M., Dweck, J. F., Mason, D. E., Hart, C. R., Buck, S. B., Peters, E. C., Agnew, B. J., & Hsieh-Wilson, L. C. (2008). Direct In-Gel Fluorescence Detection and Cellular Imaging of O -GlcNAc-Modified Proteins. Journal of the American Chemical Society. https://doi.org/10.1021/ja8030467
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