The DOI matches its Crossref record; 219 of the 219 references it cites were found in a registry or an index.
Click Chemistry in Proteomic Investigations
Open accessAbstract
Despite advances in genetic and proteomic techniques, a complete portrait of the proteome and its complement of dynamic interactions and modifications remains a lofty, and as of yet unrealized objective. Specifically, traditional biological and analytical approaches have not been able to address key questions relating to the interactions of proteins with small molecules, including drugs, drug candidates, metabolites, or protein post-translational modifications. Fortunately, chemists have bridged this experimental gap through the creation of bioorthogonal reactions. These reactions allow for the incorporation of chemical groups with highly selective reactivity into small molecules or protein modifications without perturbing their biological function, enabling the selective installation of an analysis tag for downstream investigations. The introduction of chemical strategies to parse and enrich subsets of the “functional” proteome has empowered mass spectrometry (MS)-based methods to delve more deeply and precisely into the biochemical state of cells and its perturbations by small molecules. In this Primer, we discuss how one of the most versatile bioorthogonal reactions, “click chemistry,” has been exploited to overcome limitations of biological approaches to enable the selective marking and functional investigation of critical protein/small-molecule interactions and post-translational modifications in native biological environments.
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Profiling the Proteome-Wide Selectivity of Diverse Electrophiles
Zanon, P. R. A., Yu, F., Musacchio, P., Lewald, L., Zollo, M., Krauskopf, K., Mrdović, D., Raunft, P., Maher, T. E., Cigler, M., et al. (2021) · doi:10.26434/chemrxiv.14186561.v1 · Cited by 35
Transforming Chemical Proteomics Enrichment into a High-Throughput Method Using an SP2E Workflow
Becker, T., Wiest, A., Telek, A., Bejko, D., Hoffmann-Röder, A., & Kielkowski, P. (2022) · JACS Au · doi:10.1021/jacsau.2c00284 · Cited by 33
Brain cell-specific origin of circulating microRNA biomarkers in experimental temporal lobe epilepsy
Brindley, E., Heiland, M., Mooney, C., Diviney, M., Mamad, O., Hill, T. D. M., Yan, Y., Venø, M. T., Reschke, C. R., Batool, A., et al. (2023) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2023.1230942 · Cited by 21
Metadata
- Authors
- Parker, C. G., & Pratt, M. R.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2020-02
- Volume
- 180
- Issue
- 4
- Pages
- 605-632
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W3006394146
- Cited by
- 380
- References
- 219
- FWCI
- 18.13
- Type
- journal article
- Language
- en
- Open access
- bronze
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