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Cited by 329

The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.

Lateral Diffusion of Membrane Proteins

Ramadurai, Sivaramakrishnan, Holt, Andrea, Krasnikov, Victor, van den Bogaart, Geert, Killian, J. Antoinette, Poolman, Bert·2009·Journal of the American Chemical Society
DOI 10.1021/ja902853g
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Abstract

We measured the lateral mobility of integral membrane proteins reconstituted in giant unilamellar vesicles (GUVs), using fluorescence correlation spectroscopy. Receptor, channel, and transporter proteins with 1-36 transmembrane segments (lateral radii ranging from 0.5 to 4 nm) and a alpha-helical peptide (radius of 0.5 nm) were fluorescently labeled and incorporated into GUVs. At low protein-to-lipid ratios (i.e., 10-100 proteins per microm(2) of membrane surface), the diffusion coefficient D displayed a weak dependence on the hydrodynamic radius (R) of the proteins [D scaled with ln(1/R)], consistent with the Saffman-Delbruck model. At higher protein-to lipid ratios (up to 3000 microm(-2)), the lateral diffusion coefficient of the molecules decreased linearly with increasing the protein concentration in the membrane. The implications of our findings for protein mobility in biological membranes (protein crowding of approximately 25,000 microm(-2)) and use of diffusion measurements for protein geometry (size, oligomerization) determinations are discussed.

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Metadata

Authors
Ramadurai, S., Holt, A., Krasnikov, V., van den Bogaart, G., Killian, J. A., & Poolman, B.
Venue
Journal of the American Chemical Society
Publisher
American Chemical Society (ACS)
Published
2009-08-12
Volume
131
Issue
35
Pages
12650-12656
ISSN
  • 0002-7863 (ISSN-L)
  • 1520-5126
DOI
10.1021/ja902853g
OpenAlex
W2151616366
Cited by
329
References
40
FWCI
6.24
Type
journal article
Language
en
Open access
closed

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