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Fluorogenic Probes for Multicolor Imaging in Living Cells
Open accessAbstract
Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-permeable, and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.
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Citations
Cited by
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Vicidomini, G., Bianchini, P., & Diaspro, A. (2018) · Nature Methods · doi:10.1038/nmeth.4593 · Cited by 717
A general method to fine-tune fluorophores for live-cell and in vivo imaging
Grimm, J. B., Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C., Patel, R., Lu, R., Macklin, J. J., Keller, P. J., Ji, N., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4403 · Cited by 665
A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy
Wang, L., Tran, M., D’Este, E., Roberti, J., Koch, B., Xue, L., & Johnsson, K. (2020) · Nature Chemistry · doi:10.1038/s41557-019-0371-1 · Cited by 382
Self-citationDanylchuk, D. I., Jouard, P.-H., & Klymchenko, A. S. (2021) · Journal of the American Chemical Society · doi:10.1021/jacs.0c10972 · Cited by 298
Competition for microtubule lattice spacing between a microtubule expander and compactor
Paquette, A. L., Tetlalmatzi, S. C., Haineault, J. A. G., Li, Y., Finkel, N., Hendricks, A. G., Brouhard, G. J., & Sébastien, M. (2025) · Current Biology · doi:10.1016/j.cub.2025.07.080 · Cited by 17
Metadata
- Authors
- Lukinavičius, G., Reymond, L., Umezawa, K., Sallin, O., D’Este, E., Göttfert, F., Ta, H., Hell, S. W., Urano, Y., & Johnsson, K.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2016-07-21
- Volume
- 138
- Issue
- 30
- Pages
- 9365-9368
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2474094449
- Cited by
- 281
- References
- 0
- FWCI
- 16.86
- Type
- journal article
- Language
- en
- Open access
- green
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- Lukinavičius, G., Reymond, L., Umezawa, K., Sallin, O., D’Este, E., Göttfert, F., Ta, H., Hell, S. W., Urano, Y., & Johnsson, K. (2016). Fluorogenic Probes for Multicolor Imaging in Living Cells. Journal of the American Chemical Society. https://doi.org/10.1021/jacs.6b04782
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