Controlling Light Harvesting with Light
Open accessAbstract
When exposed to intense sunlight, all organisms performing oxygenic photosynthesis implement various photoprotective strategies to prevent potentially lethal photodamage. The rapidly responding photoprotective mechanisms, occurring in the light-harvesting pigment-protein antennae, take effect within tens of seconds, while the dramatic and potentially harmful light intensity fluctuations manifest also on shorter time scales. Here we show that, upon illumination, individual phycobilisomes from Synechocystis PCC 6803, which, in vivo under low-light conditions, harvest solar energy, and have the built-in capacity to switch rapidly and reversibly into light-activated energy-dissipating states. Simultaneously measured fluorescence intensity, lifetime, and spectra, compared with a multicompartmental kinetic model, revealed that essentially any subunit of a phycobilisome can be quenched, and that the core complexes were targeted most frequently. Our results provide the first evidence for fluorescence blinking from a biologically active system at physiological light intensities and suggest that the light-controlled switches to intrinsically available energy-dissipating states are responsible for a novel type of photoprotection in cyanobacteria. We anticipate other photosynthetic organisms to employ similar strategies to respond instantly to rapid solar light intensity fluctuations. A detailed understanding of the photophysics of photosynthetic antenna complexes is of great interest for bioinspired solar energy technologies.
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Static disorder also strongly affects the fate of an excitation in a light-harvesting complex by changing the probability of the excitation to be trapped in an energy sink or by pigment clusters that are not neighbouring other complexes in the photosystem.
“Static disorder also strongly affects the fate of an excitation in a light-harvesting complex by changing the probability of the excitation to be trapped in an energy sink [63,64] or by pigment clusters that are not neighbouring other complexes in the photosystem [65].”
From The future of quantum biology (2018) · cites this paper as [64]
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Self-citation
- Authors
- Gwizdala, M., Berera, R., Kirilovsky, D., van Grondelle, R., & Krüger, T. P. J.
- Affiliations
- Vrije Universiteit Amsterdam (NL)
- Kagawa University (JP)
- Centre National de la Recherche Scientifique (FR)
- Department of Physics and Astronomy, VU Amsterdam, 1081 HV Amsterdam, The Netherlands
- University of Pretoria (ZA)
- Corresponding author
- Gwizdala, Michal
- Krüger, Tjaart P.J.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2016-09-02
- Volume
- 138
- Issue
- 36
- Pages
- 11616-11622
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2517845855
- Cited by
- 60
- References
- 38
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 5 · 2023: 5 · 2022: 7 · 2021: 5 · 2020: 5 · 2019: 7 · 2018: 8 · 2017: 10
- FWCI
- 4.33
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Locations
- Journal of the American Chemical Society (publishedVersion)
- PubMed (publishedVersion)
- VU Research Portal (publishedVersion)
- UpSpace Institutional Repository (University of Pretoria) (submittedVersion)
- HAL (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-10-INSB-05-01)
- European Research Council (267333)
- Koninklijke Nederlandse Akademie van Wetenschappen
- Japan Society for the Promotion of Science
- Centre National de la Recherche Scientifique
- University of Pretoria (A0W679)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives
- European Molecular Biology Organization
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (700.58.305)
- Phycosource
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
- Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Photochemistry and Electron Transfer Studies
- Keywords
- photoprotection, light-harvesting complexes, phycobilisomes, Synechocystis sp. PCC 6803, fluorescence blinking, energy dissipation, fluctuating light, photodamage, fluorescence lifetime, fluorescence spectra, kinetic modeling, cyanobacteria
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