The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Activatable NIR Fluorescence/MRI Bimodal Probes for in Vivo Imaging by Enzyme-Mediated Fluorogenic Reaction and Self-Assembly
Abstract
Stimuli-responsive in situ self-assembly of small molecules to form nanostructures in living subjects has produced promising tools for molecular imaging and tissue engineering. However, controlling the self-assembly process to simultaneously activate multimodality imaging signals in a small-molecule probe is challenging. In this paper, we rationally integrate a fluorogenic reaction into enzyme-responsive in situ self-assembly to design small-molecule-based activatable near-infrared (NIR) fluorescence and magnetic resonance (MR) bimodal probes for molecular imaging. Using alkaline phosphatase (ALP) as a model target, we demonstrate that probe (P-CyFF-Gd) can be activated by endogenous ALP overexpressed on cell membranes, producing membrane-localized assembled nanoparticles (NPs) that can be directly visualized by cryo-SEM. Simultaneous enhancements in NIR fluorescence (>70-fold at 710 nm) and r 1 relaxivity (∼2.3-fold) enable real-time, high-sensitivity, high-spatial-resolution imaging and localization of the ALP activity in live tumor cells and mice. P-CyFF-Gd can also delineate orthotopic liver tumor foci, facilitating efficient real-time, image-guided surgical resection of tumor tissues in intraoperative mice. This strategy combines activatable NIR fluorescence via a fluorogenic reaction and activatable MRI via in situ self-assembly to promote ALP activity imaging, which could be applicable to design other activatable bimodal probes for in vivo imaging of enzyme activity and locations in real time.
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Cites
Reaction-based small-molecule fluorescent probes for chemoselective bioimaging
Chan, J., Dodani, S. C., & Chang, C. J. (2012) · Nature Chemistry · doi:10.1038/nchem.1500 · Cited by 1,883
Chemistry of MRI Contrast Agents: Current Challenges and New Frontiers
Wahsner, J., Gale, E. M., Rodríguez-Rodríguez, A., & Caravan, P. (2019) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00363 · Cited by 1,603
Image-guided cancer surgery using near-infrared fluorescence
Vahrmeijer, A. L., Hutteman, M., van der Vorst, J. R., van de Velde, C. J. H., & Frangioni, J. V. (2013) · Nature Reviews Clinical Oncology · doi:10.1038/nrclinonc.2013.123 · Cited by 1,466
Li, J., & Pu, K. (2019) · Chemical Society Reviews · doi:10.1039/c8cs00001h · Cited by 1,168
Nanomaterials for In Vivo Imaging
Smith, B. R., & Gambhir, S. S. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00073 · Cited by 1,075
Multimodality Imaging Probes: Design and Challenges
Louie, A. (2010) · Chemical Reviews · doi:10.1021/cr9003538 · Cited by 1,044
Recent progresses in small-molecule enzymatic fluorescent probes for cancer imaging
Liu, H.-W., Chen, L., Xu, C., Li, Z., Zhang, H., Zhang, X.-B., & Tan, W. (2018) · Chemical Society Reviews · doi:10.1039/c7cs00862g · Cited by 948
Self-citationA Unique Approach to Development of Near-Infrared Fluorescent Sensors for in Vivo Imaging
Yuan, L., Lin, W., Zhao, S., Gao, W., Chen, B., He, L., & Zhu, S. (2012) · Journal of the American Chemical Society · doi:10.1021/ja305802v · Cited by 679
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Self-citationZhao, Z., Fan, H., Zhou, G., Bai, H., Liang, H., Wang, R., Zhang, X., & Tan, W. (2014) · Journal of the American Chemical Society · doi:10.1021/ja5029364 · Cited by 584
Cited by
Advanced Fluorescence Imaging Technology in the Near-Infrared-II Window for Biomedical Applications
Li, C., Chen, G., Zhang, Y., Wu, F., & Wang, Q. (2020) · Journal of the American Chemical Society · doi:10.1021/jacs.0c07022 · Cited by 1,037
Activity‐Based NIR Enzyme Fluorescent Probes for the Diagnosis of Tumors and Image‐Guided Surgery
Li, H., Kim, D., Yao, Q., Ge, H., Chung, J., Fan, J., Wang, J., Peng, X., & Yoon, J. (2021) · Angewandte Chemie International Edition · doi:10.1002/anie.202009796 · Cited by 363
Hemicyanine‐Based Near‐Infrared Activatable Probes for Imaging and Diagnosis of Diseases
Zeng, Z., Liew, S. S., Wei, X., & Pu, K. (2021) · Angewandte Chemie International Edition · doi:10.1002/anie.202107877 · Cited by 359
Qin, Z., Ren, T., Zhou, H., Zhang, X., He, L., Li, Z., Zhang, X., & Yuan, L. (2022) · Angewandte Chemie International Edition · doi:10.1002/anie.202201541 · Cited by 140
Metadata
- Authors
- Yan, R., Hu, Y., Liu, F., Wei, S., Fang, D., Shuhendler, A. J., Liu, H., Chen, H.-Y., & Ye, D.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2019-06-07
- Volume
- 141
- Issue
- 26
- Pages
- 10331-10341
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2949216521
- Cited by
- 389
- References
- 52
- FWCI
- 18.86
- Type
- journal article
- Language
- en
- Open access
- closed
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- Yan, R., Hu, Y., Liu, F., Wei, S., Fang, D., Shuhendler, A. J., Liu, H., Chen, H.-Y., & Ye, D. (2019). Activatable NIR Fluorescence/MRI Bimodal Probes for in Vivo Imaging by Enzyme-Mediated Fluorogenic Reaction and Self-Assembly. Journal of the American Chemical Society. https://doi.org/10.1021/jacs.9b03649
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