The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Real-time imaging of oxidative and nitrosative stress in the liver of live animals for drug-toxicity testing
Open accessAbstract
Current drug-safety assays for hepatotoxicity rely on biomarkers with low predictive power. The production of radical species, specifically reactive oxygen species (ROS) and reactive nitrogen species (RNS), has been proposed as an early unifying event linking the bioactivation of drugs to hepatotoxicity and as a more direct and mechanistic indicator of hepatotoxic potential. Here we present a nanosensor for rapid, real-time in vivo imaging of drug-induced ROS and RNS for direct evaluation of acute hepatotoxicity. By combining fluorescence resonance energy transfer (FRET) and chemiluminescence resonance energy transfer (CRET), our semiconducting polymer–based nanosensor simultaneously and differentially detects RNS and ROS using two optically independent channels. We imaged drug-induced hepatotoxicity and its remediation longitudinally in mice after systemic challenge with acetaminophen or isoniazid. We detected dose-dependent ROS and RNS activity in the liver within minutes of drug challenge, which preceded histological changes, protein nitration and DNA double-strand-break induction.
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Cites
Can the pharmaceutical industry reduce attrition rates?
Kola, I., & Landis, J. (2004) · Nature Reviews Drug Discovery · doi:10.1038/nrd1470 · Cited by 4,167
Second window for in vivo imaging
Smith, A. M., Mancini, M. C., & Nie, S. (2009) · Nature Nanotechnology · doi:10.1038/nnano.2009.326 · Cited by 2,848
Peroxynitrite: biochemistry, pathophysiology and development of therapeutics
Szabó, C., Ischiropoulos, H., & Radi, R. (2007) · Nature Reviews Drug Discovery · doi:10.1038/nrd2222 · Cited by 2,134
Saito, C., Zwingmann, C., & Jaeschke, H. (2010) · Hepatology · doi:10.1002/hep.23267 · Cited by 430
James, L. P. (2003) · Toxicological Sciences · doi:10.1093/toxsci/kfg181 · Cited by 244
Cited by
Ratiometric optical nanoprobes enable accurate molecular detection and imaging
Huang, X., Song, J., Yung, B. C., Huang, X., Xiong, Y., & Chen, X. (2018) · Chemical Society Reviews · doi:10.1039/c7cs00612h · Cited by 820
Jiang, Y., Li, J., Zhen, X., Xie, C., & Pu, K. (2018) · Advanced Materials · doi:10.1002/adma.201705980 · Cited by 601
Self-citationLei, Z., Sun, C., Pei, P., Wang, S., Li, D., Zhang, X., & Zhang, F. (2019) · Angewandte Chemie International Edition · doi:10.1002/anie.201904182 · Cited by 362
Qi, J., Chen, C., Zhang, X., Hu, X., Ji, S., Kwok, R. T. K., Lam, J. W. Y., Ding, D., & Tang, B. Z. (2018) · Nature Communications · doi:10.1038/s41467-018-04222-8 · Cited by 350
Green, O., Eilon, T., Hananya, N., Gutkin, S., Bauer, C. R., & Shabat, D. (2017) · ACS Central Science · doi:10.1021/acscentsci.7b00058 · Cited by 336
Metadata
- Authors
- Shuhendler, A. J., Pu, K., Cui, L., Uetrecht, J. P., & Rao, J.
- Venue
- Nature Biotechnology
- Published
- 2014-03-23
- Volume
- 32
- Issue
- 4
- Pages
- 373-380
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2068664917
- Cited by
- 623
- References
- 5
- FWCI
- 54.67
- Type
- journal article
- Language
- en
- Open access
- green
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- Shuhendler, A. J., Pu, K., Cui, L., Uetrecht, J. P., & Rao, J. (2014). Real-time imaging of oxidative and nitrosative stress in the liver of live animals for drug-toxicity testing. Nature Biotechnology. https://doi.org/10.1038/nbt.2838
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