Advanced Fluorescence Imaging Technology in the Near-Infrared-II Window for Biomedical Applications
Abstract
Fluorescence imaging has become a fundamental tool for biomedical applications; nevertheless, its intravital imaging capacity in the conventional wavelength range (400-950 nm) has been restricted by its extremely limited tissue penetration. To tackle this challenge, a novel imaging approach using the fluorescence in the second near-infrared window (NIR-II, 1000-1700 nm) has been developed in the past decade to achieve deep penetration and high-fidelity imaging, and thus significant biomedical applications have begun to emerge. In this Perspective, we first examine recent discoveries and challenges in the development of novel NIR-II fluorophores and compatible imaging apparatuses. Subsequently, the recent advances in bioimaging, biosensing, and therapy using such a cutting-edge imaging technique are highlighted. Finally, based on the achievement in the representative studies, we elucidate the main concerns regarding this imaging technique and give some advice and prospects for the development of NIR-II imaging for future biomedical applications.
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Self-citation
- Authors
- Li, C., Chen, G., Zhang, Y., Wu, F., & Wang, Q.
- Affiliations
- Chinese Academy of Sciences (CN)
- University of Science and Technology of China (CN)
- Corresponding author
- Wang, Qiangbin
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2020-08-07
- Volume
- 142
- Issue
- 35
- Pages
- 14789-14804
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W3047785641
- Cited by
- 1,037
- References
- 119
- Citations by year
- 2026: 120 · 2025: 172 · 2024: 227 · 2023: 214 · 2022: 156 · 2021: 129 · 2020: 19
- FWCI
- 19.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of the American Chemical Society (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Science and Technology of the People's Republic of China (2016YFA0101503, 2017YFA0205503)
- Youth Innovation Promotion Association of the Chinese Academy of Sciences
- National Natural Science Foundation of China (21703282, 21778070, 21934007)
- Chinese Academy of Sciences (XDB36000000, ZDBS-LY-SLH021)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- NIR-II fluorescence imaging, second near-infrared window, NIR-II fluorophores, deep tissue penetration, intravital imaging, biosensing, photothermal therapy, bioimaging
- MeSH terms
- Animals, Fluorescent Dyes, Humans, Infrared Rays, Biomedical Research, Optical Imaging
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