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Determination of Flavonoids in Plant Material by HPLC with Diode-Array and Electro-Array Detections
Abstract
A high-performance liquid chromatographic (HPLC) method with in-line connected diode-array (DAD) and electro-array (EC) detection to identify and quantify 17 flavonoids in plant-derived foods is described. Catechins were extracted from the samples using ethyl acetate, and quantification of these compounds was performed with the EC detector. Other flavonoids were quantified with DAD after acid hydrolysis. The methods developed were effective for the determination of catechins and other flavonoids in plant-derived foods. Responses of the detection systems were linear within the range evaluated, 20-200 ng/injection (DAD) and 20-100 ng/injection (EC), with correlation coefficients exceeding 0.999. Coefficient of variation was under 10.5%, and recoveries of flavonoids ranged from 70 to 124%. Purity of the flavonoid peaks was confirmed by combining the spectral and voltammetric data.
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Cited by
Concentrations of Proanthocyanidins in Common Foods and Estimations of Normal Consumption
Kelm, M. A., Hammerstone, J. F., Beecher, G., Holden, J., Haytowitz, D., Gebhardt, S., Gu, L., & Prior, R. L. (2004) · The Journal of Nutrition · doi:10.1093/jn/134.3.613 · Cited by 938
Review of methods to determine chain-breaking antioxidant activity in food
ROGINSKY, V, & LISSI, E. (2005) · Food Chemistry · doi:10.1016/j.foodchem.2004.08.004 · Cited by 855
Contents of vitamins, mineral elements, and some phenolic compounds in cultivated mushrooms
Mattila, P., Könkö, K., Eurola, M., Pihlava, J.-M., Astola, J., Vahteristo, L., Hietaniemi, V., Kumpulainen, J., Valtonen, M., & Piironen, V. (2001) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf001525d · Cited by 781
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Favorable effects of berry consumption on platelet function, blood pressure, and HDL cholesterol
Erlund, I., Koli, R., Alfthan, G., Marniemi, J., Puukka, P., Mustonen, P., Mattila, P., & Jula, A. (2008) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/87.2.323 · Cited by 445
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Metadata
- Authors
- Mattila, P., Astola, J., & Kumpulainen, J.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-10-28
- Volume
- 48
- Issue
- 12
- Pages
- 5834-5841
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2041692462
- Cited by
- 380
- References
- 0
- FWCI
- 2.92
- Type
- journal article
- Language
- en
- Open access
- closed
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- Mattila, P., Astola, J., & Kumpulainen, J. (2000). Determination of Flavonoids in Plant Material by HPLC with Diode-Array and Electro-Array Detections. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf000661f
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