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High-Throughput Assay of Oxygen Radical Absorbance Capacity (ORAC) Using a Multichannel Liquid Handling System Coupled with a Microplate Fluorescence Reader in 96-Well Format
Abstract
The oxygen radical absorbance capacity (ORAC) assay has been widely accepted as a standard tool to measure the antioxidant activity in the nutraceutical, pharmaceutical, and food industries. However, the ORAC assay has been criticized for a lack of accessibility due to the unavailability of the COBAS FARA II analyzer, an instrument discontinued by the manufacturer. In addition, the manual sample preparation is time-consuming and labor-intensive. The objective of this study was to develop a high-throughput instrument platform that can fully automate the ORAC assay procedure. The new instrument platform consists of a robotic eight-channel liquid handling system and a microplate fluorescence reader. By using the high-throughput platform, the efficiency of the assay is improved with at least a 10-fold increase in sample throughput over the current procedure. The mean of intra- and interday CVs was
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Cited by
The Chemistry behind Antioxidant Capacity Assays
Huang, D., Ou, B., & Prior, R. L. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf030723c · Cited by 6,162
Self-citationAntioxidants and antioxidant methods: an updated overview
Gulcin, İ. (2020) · Archives of Toxicology · doi:10.1007/s00204-020-02689-3 · Cited by 2,205
Fluorescence probes used for detection of reactive oxygen species
Gomes, A., Fernandes, E., & Lima, J. L. F. C. (2005) · Journal of Biochemical and Biophysical Methods · doi:10.1016/j.jbbm.2005.10.003 · Cited by 1,844
Prior, R. L., Hoang, H., Gu, L., Wu, X., Bacchiocca, M., Howard, L., Hampsch-Woodill, M., Huang, D., Ou, B., & Jacob, R. (2003) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0262256 · Cited by 1,455
Self-citationExtending Applicability of the Oxygen Radical Absorbance Capacity (ORAC−Fluorescein) Assay
Dávalos, A., Gómez-Cordovés, C., & Bartolomé, B. (2004) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0305231 · Cited by 1,207
Metadata
- Authors
- Huang, D., Ou, B., Hampsch-Woodill, M., Flanagan, J. A., & Prior, R. L.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-07-01
- Volume
- 50
- Issue
- 16
- Pages
- 4437-4444
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2090678272
- Cited by
- 1,566
- References
- 0
- FWCI
- 5.24
- Type
- journal article
- Language
- en
- Open access
- closed
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- Huang, D., Ou, B., Hampsch-Woodill, M., Flanagan, J. A., & Prior, R. L. (2002). High-Throughput Assay of Oxygen Radical Absorbance Capacity (ORAC) Using a Multichannel Liquid Handling System Coupled with a Microplate Fluorescence Reader in 96-Well Format. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf0201529
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