The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Assays for Hydrophilic and Lipophilic Antioxidant Capacity (oxygen radical absorbance capacity (ORACFL)) of Plasma and Other Biological and Food Samples
Abstract
Methods are described for the extraction and analysis of hydrophilic and lipophilic antioxidants, using modifications of the oxygen radical absorbing capacity (ORAC(FL)) procedure. These methods provide, for the first time, the ability to obtain a measure of "total antioxidant capacity" in the protein free plasma, using the same peroxyl radical generator for both lipophilic and hydrophilic antioxidants. Separation of the lipophilic and hydrophilic antioxidant fractions from plasma was accomplished by extracting with hexane after adding water and ethanol to the plasma (hexane/plasma/ethanol/water, 4:1:2:1, v/v). Lipophilic and hydrophilic antioxidants were efficiently partitioned between hexane and aqueous solvents. Conditions for controlling temperature effects and decreasing assay variability using fluorescein as the fluorescent probe were validated in different laboratories. Incubation (37 degrees C for at least 30 min) of the buffer to which AAPH was dissolved was critical in decreasing assay variability. Lipophilic antioxidants represented 33.1 +/- 1.5 and 38.2 +/- 1.9% of the total antioxidant capacity of the protein free plasma in two independent studies of 6 and 10 subjects, respectively. Methods are described for application of the assay techniques to other types of biological and food samples.
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Cites
Ou, B., Hampsch-Woodill, M., & Prior, R. L. (2001) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf010586o · Cited by 2,932
Self-citationHuang, D., Ou, B., Hampsch-Woodill, M., Flanagan, J. A., & Prior, R. L. (2002) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0201529 · Cited by 1,566
Self-citationAntioxidant Capacity of Tea and Common Vegetables
Cao, G., Sofic, E., & Prior, R. L. (1996) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf9602535 · Cited by 1,295
Self-citationIn vivo total antioxidant capacity: comparison of different analytical methods1
Prior, R. L., & Cao, G. (1999) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(99)00203-8 · Cited by 925
Self-citationComparison of different analytical methods for assessing total antioxidant capacity of human serum
Cao, G., & Prior, R. L. (1998) · Clinical Chemistry · doi:10.1093/clinchem/44.6.1309 · Cited by 902
Self-citationHuang, D., Ou, B., Hampsch-Woodill, M., Flanagan, J. A., & Deemer, E. K. (2002) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0113732 · Cited by 518
Self-citation[5]Measurement of oxygen radical absorbance capacity in biological samples
Cao, G., & Prior, R. L. (1999) · Methods in Enzymology · doi:10.1016/s0076-6879(99)99008-0 · Cited by 506
Self-citationCao, G., Russell, R. M., Lischner, N., & Prior, R. L. (1998) · The Journal of Nutrition · doi:10.1093/jn/128.12.2383 · Cited by 502
Self-citationA fluorescence-based method for measuring total plasma antioxidant capability
Ghiselli, A., Serafini, M., Maiani, G., Azzini, E., & Ferro-Luzzi, A. (1995) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(94)00102-p · Cited by 441
Cao, G., Booth, S. L., Sadowski, J. A., & Prior, R. L. (1998) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/68.5.1081 · Cited by 402
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Cited by
The Chemistry behind Antioxidant Capacity Assays
Huang, D., Ou, B., & Prior, R. L. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf030723c · Cited by 6,162
Self-citationFluorescence probes used for detection of reactive oxygen species
Gomes, A., Fernandes, E., & Lima, J. L. F. C. (2005) · Journal of Biochemical and Biophysical Methods · doi:10.1016/j.jbbm.2005.10.003 · Cited by 1,844
Biological Activities of Polyphenols from Grapes
Xia, E.-Q., Deng, G.-F., Guo, Y.-J., & Li, H.-B. (2010) · International Journal of Molecular Sciences · doi:10.3390/ijms11020622 · Cited by 1,063
Review of methods to determine antioxidant capacities
Karadag, A., Ozcelik, B., & Saner, S. (2009) · Food Analytical Methods · doi:10.1007/s12161-008-9067-7 · Cited by 753
Apak, R., Gorinstein, S., Böhm, V., Schaich, K. M., Özyürek, M., & Güçlü, K. (2013) · Pure and Applied Chemistry · doi:10.1351/pac-rep-12-07-15 · Cited by 586
Metadata
- Authors
- Prior, R. L., Hoang, H., Gu, L., Wu, X., Bacchiocca, M., Howard, L., Hampsch-Woodill, M., Huang, D., Ou, B., & Jacob, R.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-04-29
- Volume
- 51
- Issue
- 11
- Pages
- 3273-3279
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2050385572
- Cited by
- 1,455
- References
- 20
- FWCI
- 12.71
- Type
- journal article
- Language
- en
- Open access
- closed
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- Prior, R. L., Hoang, H., Gu, L., Wu, X., Bacchiocca, M., Howard, L., Hampsch-Woodill, M., Huang, D., Ou, B., & Jacob, R. (2003). Assays for Hydrophilic and Lipophilic Antioxidant Capacity (oxygen radical absorbance capacity (ORACFL)) of Plasma and Other Biological and Food Samples. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf0262256
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