The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Extending Applicability of the Oxygen Radical Absorbance Capacity (ORAC−Fluorescein) Assay
Abstract
The ORAC-fluorescein (ORAC-FL) method recently validated using automatic liquid handling systems has now been adapted to manual handling and using a conventional fluorescence microplate reader. As calculated for Trolox, the precision of the method was <3.0, expressed as percent coefficient of variation. The accuracy of the method was <2.3, expressed as percent variation of the mean. The detection and quantification limits were those corresponding to 0.5- and 1-microM Trolox standard solutions, respectively. The method has been applied to 10 pure compounds (benzoic and cinnamic acids and aldehydes, flavonoids, and butylated hydroxyanisole), to 30 white, rose, and bottled- and oak-aged red wines, and to 7 commercial dietary antioxidant supplements. All samples exhibited a good linear response with concentration. As seen by other methodologies, the chemical structure of a compound determines its antioxidant activity (ORAC-FL value). Of particular interest were the results with oak-aged red wines from different vintages (1989-2002) that confirm influence of vintage, but not origin of the oak, in the antioxidant activity of wines from the same variety. Dietary antioxidant supplements presented a great variability (170-fold difference) in their antioxidant potency. This work proves applicability of the ORAC-FL assay in evaluating the antioxidant activity of diverse food samples.
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Cites
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Cited by
Hernández-Ledesma, B., Dávalos, A., Bartolomé, B., & Amigo, L. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf048626m · Cited by 634
Self-citationAntioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis
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Self-citationAntioxidant evaluation protocols: Food quality or health effects
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Facts about the formation of new antioxidants in natural samples after subcritical water extraction
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Metadata
- Authors
- Dávalos, A., Gómez-Cordovés, C., & Bartolomé, B.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-12-16
- Volume
- 52
- Issue
- 1
- Pages
- 48-54
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2057434602
- Cited by
- 1,207
- References
- 30
- FWCI
- 5.52
- Type
- journal article
- Language
- en
- Open access
- closed
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