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Cited by 124Fatty Acid Research and HealthInflammatory mediators and NSAID effectsEdible Oils Quality and Analysis

Contribution of Conjugated Linoleic Acid to the Suppression of Inflammatory Responses through the Regulation of the NF-κB Pathway

Cheng, Wen-Ling, Lii, Chong-Kuei, Chen, Haw-Wen, Lin, Ting-Hui, Liu, Kai-Li·2004·Journal of Agricultural and Food Chemistry
DOI 10.1021/jf0348626
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Abstract

Data from a number of researchers have shown that conjugated linoleic acid (CLA) has some beneficial health activities in animal models. Because inflammatory responses are associated with pathophysiology of many diseases, the aim of this study is to explore the effect and mechanism of CLA in the regulation of lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 macrophages. The addition of increasing levels of CLA proportionally augmented the incorporation of CLA in cultures. CLA diminished LPS-induced mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2) as well as subsequent production of nitric oxide and prostaglandin E(2), respectively. We further examined the effect of CLA on LPS-induced NF-kappaB activation by Western blot and the electrophoretic mobility shift assay. The addition of CLA at 200 microM significantly diminished LPS-induced protein expression of the cytoplasmic phosphorylated inhibitor kappaBalpha and nuclear p65 as well as NF-kappaB nuclear protein-DNA binding affinity. In conclusion, our data suggest that CLA may inhibit LPS-induced inflammatory events in RAW 264.7 macrophages and this inhibitory activity of CLA, at least in part, occurs through CLA modulating the NF-kappaB activation and therefore negatively regulating expression of inflammatory mediators.

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Crossref · 10.1021/jf0348626

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  • cLA consists of positional isomers of linoleic acid having conjugated dienes in the cis and trans configurations.

    “cLA consists of positional isomers of linoleic acid having conjugated dienes in the cis and trans configurations [39], [51], [52].”

    From Olives and olive oil are sources of electrophilic fatty acid nitroalkenes (2014) · cites this paper as [52]

  • Conjugated linoleic acid displays immune-modulatory and anti-inflammatory effects, with proposed mechanisms including reduction of pro-inflammatory cytokine levels via inhibition of NF-κB-dependent gene expression and activation of PPAR-regulated gene expression.

    “Conjugated linoleic acid displays immune-modulatory and anti-inflammatory effects [51], [52], with the mechanisms accounting for these actions proposed to include the reduction of pro-inflammatory cytokine levels via inhibition of NF-κB-dependent gene expression and activation of PPAR-regulated gene expression [51], [52].”

    From Olives and olive oil are sources of electrophilic fatty acid nitroalkenes (2014) · cites this paper as [52]

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Authors
Cheng, W.-L., Lii, C.-K., Chen, H.-W., Lin, T.-H., & Liu, K.-L.
Affiliations
Chung Shan Medical University (TW)
Corresponding author
Liu, Kai-Li
ORCID
Venue
Journal of Agricultural and Food Chemistry
Publisher
American Chemical Society (ACS)
Published
2003-12-10
Volume
52
Issue
1
Pages
71-78
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
DOI
10.1021/jf0348626
OpenAlex
W2094519127
Cited by
124
References
32
Citations by year
2024: 1 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 6 · 2019: 2 · 2018: 3 · 2017: 3 · 2016: 2 · 2015: 4 · 2014: 7 · 2013: 9 · 2012: 5
FWCI
8.59
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Agricultural and Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Health Sciences › Medicine › Pharmacology › Inflammatory mediators and NSAID effects
  • Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
Keywords
conjugated linoleic acid, NF-κB signaling, lipopolysaccharide-induced inflammation, RAW 264.7 macrophages, inducible nitric oxide synthase, cyclooxygenase-2, nitric oxide, prostaglandin E2, IκBα phosphorylation, p65 nuclear translocation, anti-inflammatory activity
MeSH terms
Animals, Anti-Inflammatory Agents, Cell Line, Cell Survival, DNA, Fatty Acids, Isoenzymes, Lipopolysaccharides, Macrophages, Nitrites, Prostaglandin-Endoperoxide Synthases, RNA, Messenger, Dinoprostone, NF-kappa B, Nitric Oxide Synthase, Linoleic Acids, Conjugated, Mice, Cyclooxygenase 2, Nitric Oxide Synthase Type II

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