Cited by 166Coffee research and impactsPotato Plant ResearchFood Chemistry and Fat Analysis

Melanoidins from Coffee Infusions. Fractionation, Chemical Characterization, and Effect of the Degree of Roast

Nunes, Fernando M., Coimbra, Manuel A.·2007·Journal of Agricultural and Food Chemistry

Abstract

A method involving fractionation in ethanol aqueous solutions, anion exchange chromatography, and immobilized copper chelating chromatography was developed to obtain high molecular weight anionic melanoidin populations from coffee infusions. Six anionic fractions with different physicochemical properties (ethanol solubility and chelating ability) and chemical composition regarding carbohydrate as well as protein nature and content were isolated. Fractions with similar chemical composition were obtained for light-, medium-, and dark-roasted coffee infusions. These melanoidin fractions accounted for 30-33% of the cold-water soluble high molecular weight material, independently of the degree of roast in coffee. The nature and abundance of the different polysaccharides in each fraction were dependent on their ethanol solubility. The 50% ethanol insoluble melanoidin populations contained mostly galactomannan-like carbohydrates, and the fractions obtained with 75% ethanol contained mostly arabinogalactan-like carbohydrates. The melanoidin populations with chelating properties presented significantly lower carbohydrate content and, from these, the 75% ethanol soluble fractions were almost devoid of carbohydrate material. The results obtained suggest that the chelating ability of these coffee melanoidins is modulated by their carbohydrates.

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Crossref · 10.1021/jf063735h

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Authors
Nunes, F. M., & Coimbra, M. A.
Affiliations
University of Trás-os-Montes and Alto Douro (PT)
Corresponding author
Nunes, Fernando M.
Venue
Journal of Agricultural and Food Chemistry
Published
2007-04-25
Volume
55
Issue
10
Pages
3967-3977
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2068221219
Cited by
166
References
33
Citations by year
2026: 5 · 2025: 14 · 2024: 13 · 2023: 7 · 2022: 11 · 2021: 9 · 2020: 7 · 2019: 8 · 2018: 11 · 2017: 7 · 2016: 3 · 2015: 5 · 2014: 16 · 2013: 12 · 2012: 6
FWCI
5.10
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Agricultural and Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Pharmacology › Coffee research and impacts
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Potato Plant Research
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food Chemistry and Fat Analysis
Keywords
melanoidins, coffee infusion, ethanol solubility, polysaccharide content
MeSH terms
Anions, Carbohydrates, Chelating Agents, Coffee, Chemical Fractionation, Hot Temperature, Phenols, Polymers, Maillard Reaction

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