Chemical Characterization and Antioxidant Properties of Coffee Melanoidins
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Melanoidins, the brown polymers formed through Maillard reaction during coffee roasting, constitute up to 25% of the coffee beverages' dry matter. In this study chemical characterization of melanoidins obtained from light-, medium-, and dark-roasted coffee beans, manufactured from the same starting material, was performed. Melanoidins were separated by gel filtration chromatography and studied by MALDI-TOF mass spectrometry. Results showed that the amount of melanoidins present in the brews increased as the intensity of the thermal treatment increased, while their molecular weight decreased. The antioxidant activity of melanoidins isolated from the different brews was studied by using different methodologies. Melanoidins antiradical activity determined by ABTS(*)(+) and DMPD(*)(+) assays decreased as the intensity of roasting increased, but the ability to prevent linoleic acid peroxidation was higher in the dark-roasted samples. Data suggest that melanoidins must be carefully considered when the relevance of coffee intake in human health is studied.
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- Authors
- Borrelli, R. C., Visconti, A., Mennella, C., Anese, M., & Fogliano, V.
- Affiliations
- University of Udine (IT)
- Corresponding author
- Fogliano, Vincenzo
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-09-20
- Volume
- 50
- Issue
- 22
- Pages
- 6527-6533
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1988317209
- Cited by
- 384
- References
- 21
- Citations by year
- 2026: 8 · 2025: 11 · 2024: 14 · 2023: 18 · 2022: 14 · 2021: 15 · 2020: 22 · 2019: 15 · 2018: 24 · 2017: 17 · 2016: 25 · 2015: 22 · 2014: 25 · 2013: 21 · 2012: 20
- FWCI
- 3.63
- Type
- journal article
- Language
- en
- Open access
- green
- research.wur.nl
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Health Sciences › Medicine › Pharmacology › Coffee research and impacts
- Life Sciences › Agricultural and Biological Sciences › Food Science › Potato Plant Research
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- Maillard reaction, coffee roasting, antioxidant activity, free radical scavenging, linoleic acid peroxidation, molecular weight, coffee brews
- MeSH terms
- Antioxidants, Chromatography, Gel, Coffee, Hot Temperature, Molecular Weight, Polymers, Maillard Reaction, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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