The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Re-evaluation of the 2,2-Diphenyl-1-picrylhydrazyl Free Radical (DPPH) Assay for Antioxidant Activity
Abstract
Kinetics and stoichiometry of reactions between the 2,2-diphenyl-1-picrylhydrazyl (DPPH) stable radical and 25 antioxidant compounds with different structure, molecular weight, number of -OH groups, and redox potential were investigated by recording the loss of DPPH(•) absorbance at 515 nm continuously for 10 min. A series of antioxidant concentrations was tested to determine linear response ranges and reaction saturation points. The primary feature distinguishing antioxidant activity--rate of initial reaction (<30 s)--was controlled by whether the dominant antioxidant mechanism was electron (very fast) or hydrogen atom (slow) transfer and by impairment of steric accessibility to the DPPH radical site by bulky ring adducts and multiple phenolic rings. Results raise serious questions regarding application of the DPPH assay for ranking antioxidants and natural extracts and suggest possible redirection of this assay to distinguish active reaction mechanisms by comparing reactions rates and patterns in different solvents and in 50% water/methanol mixtures at different pH values.
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Cites
Use of a Free Radical Method to Evaluate Antioxidant Activity
Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995) · LWT - Food Science and Technology · doi:10.1016/s0023-6438(95)80008-5 · Cited by 25,994
Antioxidant Determinations by the Use of a Stable Free Radical
BLOIS, M. S. (1958) · Nature · doi:10.1038/1811199a0 · Cited by 12,994
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Self-citationGenesis and development of DPPH method of antioxidant assay
Kedare, S. B., & Singh, R. P. (2011) · Journal of Food Science and Technology · doi:10.1007/s13197-011-0251-1 · Cited by 2,139
A procedure to measure the antiradical efficiency of polyphenols
Sánchez-Moreno, C., Larrauri, J. A., & Saura-Calixto, F. (1998) · Journal of the Science of Food and Agriculture · doi:10.1002/(sici)1097-0010(199802)76:2<270::aid-jsfa945>3.0.co;2-9 · Cited by 2,010
Kinetics and Mechanisms of Antioxidant Activity using the DPPH.Free Radical Method
Bondet, V., Brand-Williams, W., & Berset, C. (1997) · LWT - Food Science and Technology · doi:10.1006/fstl.1997.0240 · Cited by 1,342
Antioxidant and Antiradical Activities of Flavonoids
Burda, S., & Oleszek, W. (2001) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf001413m · Cited by 1,221
Estimation of antiradical properties of antioxidants using DPPH assay: A critical review and results
Mishra, K., Ojha, H., & Chaudhury, N. K. (2012) · Food Chemistry · doi:10.1016/j.foodchem.2011.07.127 · Cited by 1,093
Vitamin C Equivalent Antioxidant Capacity (VCEAC) of Phenolic Phytochemicals
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Cited by
Analytical Methods Used in Determining Antioxidant Activity: A Review
Munteanu, I. G., & Apetrei, C. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22073380 · Cited by 2,119
Schaich, K. M., Tian, X., & Xie, J. (2015) · Journal of Functional Foods · doi:10.1016/j.jff.2015.01.043 · Cited by 600
Self-citationFlieger, J., Flieger, W., Baj, J., & Maciejewski, R. (2021) · Materials · doi:10.3390/ma14154135 · Cited by 441
Phenolic Melatonin-Related Compounds: Their Role as Chemical Protectors against Oxidative Stress
Galano, A., Castañeda-Arriaga, R., Pérez-González, A., Tan, D.-X., & Reiter, R. (2016) · Molecules · doi:10.3390/molecules21111442 · Cited by 60
Morone, J., Lopes, G., Preto, M., Vasconcelos, V., & Martins, R. (2020) · Marine Drugs · doi:10.3390/md18090486 · Cited by 58
“A volume of 25 μL of extract serial dilutions was mixed with 200 μL of 100 μM DPPH• reagent, freshly prepared in methanol [58] in a 96-well plate.”
Method
Metadata
- Authors
- Xie, J., & Schaich, K. M.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2014-05-05
- Volume
- 62
- Issue
- 19
- Pages
- 4251-4260
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2323547402
- Cited by
- 494
- References
- 34
- FWCI
- 13.15
- Type
- journal article
- Language
- en
- Open access
- closed
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- Xie, J., & Schaich, K. M. (2014). Re-evaluation of the 2,2-Diphenyl-1-picrylhydrazyl Free Radical (DPPH) Assay for Antioxidant Activity. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf500180u
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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