The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Hurdles and pitfalls in measuring antioxidant efficacy: A critical evaluation of ABTS, DPPH, and ORAC assays
Open accessAbstract
Assays developed to measure radical scavenging ability of natural compounds have been used as a basis for ranking and recommending best foods for consumption. However, assays often were adapted for screening assays with inadequate consideration of reaction chemistry, particularly kinetics. Recent research results raise serious questions about the chemistry, execution, and application of these assays. This paper critically evaluates conceptual and technical issues that limit use and compromise validity of three commonly-used assays – TEAC/ABTS•+, DPPH, and ORAC. Recommendations are made for discontinuing use of ABTS•+ and DPPH radicals for measuring radical quenching, redirecting them instead to distinguishing electron transfer reaction mechanisms. Conditions required for accurate results in ORAC are reviewed, and recommendations are made for redirecting this assay to distinguishing compounds that quench radicals by hydrogen atom transfer. The mechanistic information so gained can be then applied to understanding how natural antioxidants can be used most effectively in foods.
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Cites
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Cited by
Analytical Methods Used in Determining Antioxidant Activity: A Review
Munteanu, I. G., & Apetrei, C. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22073380 · Cited by 2,119
Functional Foods: Product Development, Technological Trends, Efficacy Testing, and Safety
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Metadata
- Authors
- Schaich, K. M., Tian, X., & Xie, J.
- Venue
- Journal of Functional Foods
- Publisher
- Elsevier BV
- Published
- 2015-04
- Volume
- 14
- Pages
- 111-125
- ISSN
- 1756-4646 (ISSN-L)
- OpenAlex
- W1975747074
- Cited by
- 600
- References
- 39
- FWCI
- 22.07
- Type
- journal article
- Language
- en
- Open access
- green
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- Schaich, K. M., Tian, X., & Xie, J. (2015). Hurdles and pitfalls in measuring antioxidant efficacy: A critical evaluation of ABTS, DPPH, and ORAC assays. Journal of Functional Foods. https://doi.org/10.1016/j.jff.2015.01.043
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