Chondroitin sulfate extracted from ascidian tunic inhibits phorbol ester-induced expression of inflammatory factors VCAM-1 and COX-2 by blocking NF-κB activation in mouse skin
Abstract
Inflammatory factors are known to play a key role in promoting tumorigenesis; therefore, it is a promising strategy to inhibit the inflammation for cancer prevention. The current study was performed to investigate the potential effects of chondroitin sulfate (CS) extracted from ascidian tunic on the expression of inflammatory factors induced by treatment with 12- O-tetradecanoylphorbol-13-acetate (TPA) and to elucidate the underlying molecular mechanism of CS action in mouse skin inflammation. TPA was topically applied to the shaven backs of ICR mice with or without CS (1 or 2 mg) for 4 h. The results demonstrated that CS suppressed TPA-induced edema and reduced the expression of cyclooxygenase-2, vascular cell adhesion molecule-1, and Akt signaling in mouse skin. These studies suggest that CS from ascidian tunic may be developed as an effective natural anti-inflammatory agent.
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Chondroitin sulfate isolated from ascidian Styela clava inhibited phorbol ester- and TNF-α-induced expression of inflammatory factors VCAM-1, COX-2 and iNOS by blocking Akt/NF-κB activation in mouse skin.
“Chondroitin sulfate isolated from ascidian Styela clava inhibited phorbol ester- and TNF-α-induced expression of inflammatory factors VCAM-1, COX-2 and iNOS by blocking Akt/NF-κB activation in mouse skin [154,155].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [154]
Cites
Coussens, L. M., & Werb, Z. (2002) · Nature · doi:10.1038/nature01322 · Cited by 15,111 · see citing works
Current protocols in molecular biology
Sargent, T. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90147-9 · Cited by 7,281 · see citing works
THE NF-κB AND IκB PROTEINS: New Discoveries and Insights
Baldwin, A. S. (1996) · Annual Review of Immunology · doi:10.1146/annurev.immunol.14.1.649 · Cited by 5,882 · see citing works
Activators and target genes of Rel/NF-κB transcription factors
Pahl, H. L. (1999) · Oncogene · doi:10.1038/sj.onc.1203239 · Cited by 3,727 · see citing works
Activators and Target Genes of Rel/NF-кB Transcription Factors
Loop, T., & Pahl, H. L. (2003) · Springer eBooks · doi:10.1007/978-94-010-0163-2_1 · Cited by 3,179 · see citing works
IKKβ Links Inflammation and Tumorigenesis in a Mouse Model of Colitis-Associated Cancer
Greten, F. R., Eckmann, L., Greten, T. F., Park, J. M., Li, Z.-W., Egan, L. J., Kagnoff, M. F., & Karin, M. (2004) · Cell · doi:10.1016/j.cell.2004.07.013 · Cited by 2,433 · see citing works
NF-κB functions as a tumour promoter in inflammation-associated cancer
Pikarsky, E., Porat, R., Stein, I., Abramovitch, R., Amit, S., Kasem, S., Gutkovich-Pyest, E., Urieli-Shoval, S., Galun, E., & Ben-Neriah, Y. (2004) · Nature · doi:10.1038/nature02924 · Cited by 2,295 · see citing works
Collins, T., Read, M. A., Neish, A. S., Whitley, M. Z., Thanos, D., & Maniatis, T. (1995) · The FASEB Journal · doi:10.1096/fasebj.9.10.7542214 · Cited by 1,718 · see citing works
Nuclear factor-κB: The enemy within
Aggarwal, B. (2004) · Cancer Cell · doi:10.1016/j.ccr.2004.09.003 · Cited by 1,289 · see citing works
How NF-κB is activated: the role of the IκB kinase (IKK) complex
Karin, M. (1999) · Oncogene · doi:10.1038/sj.onc.1203219 · Cited by 935 · see citing works
Nuclear factor-?B: its role in health and disease
Kumar, A., Takada, Y., Boriek, A., & Aggarwal, B. (2004) · Journal of Molecular Medicine · doi:10.1007/s00109-004-0555-y · Cited by 933 · see citing works
Clinical, Cellular, and Molecular Aspects of Cancer Invasion
Mareel, M., & Leroy, A. (2003) · Physiological Reviews · doi:10.1152/physrev.00024.2002 · Cited by 521 · see citing works
Naturally Occurring NF-κB Inhibitors
Nam, N. (2006) · Mini-Reviews in Medicinal Chemistry · doi:10.2174/138955706777934937 · Cited by 436 · see citing works
Kim, S. O., Kundu, J. K., Shin, Y. K., Park, J., Cho, M., Kim, T., & Surh, Y. (2005) · Oncogene · doi:10.1038/sj.onc.1208446 · Cited by 296 · see citing works
Transcriptional Regulation of Cyclooxygenase-2 in Mouse Skin Carcinoma Cells
Kim, Y., & Fischer, S. M. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.42.27686 · Cited by 197 · see citing works
Osakabe, N., Takano, H., Sanbongi, C., Yasuda, A., Yanagisawa, R., Inoue, K., & Yoshikawa, T. (2004) · BioFactors · doi:10.1002/biof.552210125 · Cited by 162 · see citing works
Ding, Y.-B. (2003) · World Journal of Gastroenterology · doi:10.3748/wjg.v9.i7.1409 · Cited by 159 · see citing works
Murakawa, M., Yamaoka, K., Tanaka, Y., & Fukuda, Y. (2006) · Biochemical Pharmacology · doi:10.1016/j.bcp.2006.01.005 · Cited by 154 · see citing works
Kundu, J. K., & Surh, Y. (2005) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2005.04.019 · Cited by 144 · see citing works
St-Germain, M.-É., Gagnon, V., Parent, S., & Asselin, É. (2004) · Molecular Cancer · doi:10.1186/1476-4598-3-7 · Cited by 109 · see citing works
Legendre, F., Baugé, C., Roche, R. I., Saurel, A.-S., & Pujol, J. ‐. (2007) · Osteoarthritis and Cartilage · doi:10.1016/j.joca.2007.05.020 · Cited by 70 · see citing works
Hwang, D.-M., Kundu, J. K., Shin, J., Lee, J.-C., Lee, H. W., & Surh, Y. (2006) · Carcinogenesis · doi:10.1093/carcin/bgl151 · Cited by 65 · see citing works
NF-κB p65 transactivation domain is involved in the NF-κB-inducing kinase pathway
Jiang, X., Takahashi, N., Ando, K., Otsuka, T., Tetsuka, T., & Okamoto, T. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(03)00011-1 · Cited by 57 · see citing works
Kim, H. W., Park, I., Cho, C. S., Lee, K. H., Beck, G. R., Colburn, N. H., & Cho, M. (2004) · Cancer Research · doi:10.1158/0008-5472.can-04-1231 · Cited by 56 · see citing works
Lin, W., Luo, S., Lee, C., Wang, C.-C., Wang, J., & Yang, C. (2007) · Cellular Signalling · doi:10.1016/j.cellsig.2007.01.009 · Cited by 44 · see citing works
Tung, W., Sun, C., Hsieh, H., Wang, S., Horng, J., & Yang, C. (2007) · Cellular Signalling · doi:10.1016/j.cellsig.2007.06.009 · Cited by 30 · see citing works
In Vitro Effects of Chondroitin Sulfate
Fioravanti, A., & Collodel, G. (2006) · Advances in pharmacology · doi:10.1016/s1054-3589(05)53022-9 · Cited by 26 · see citing works
The use of inflammation by tumor cells
Arias, J. I., Aller, M., & Árias, J. (2005) · Cancer · doi:10.1002/cncr.21165 · Cited by 18 · see citing works
Lı, T., Liu, S., Liu, Z., & Hu, X. (2005) · Archiv der Pharmazie · doi:10.1002/ardp.200500975 · Cited by 8 · see citing works
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Cited by
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Evolution of glycosaminoglycans
Yamada, S., Sugahara, K., & Özbek, S. (2011) · Communicative & Integrative Biology · doi:10.4161/cib.4.2.14547 · Cited by 225 · see citing works
Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects
Fedorov, S., Ermakova, S., Zvyagintseva, T., & Stonik, V. (2013) · Marine Drugs · doi:10.3390/md11124876 · Cited by 189 · see citing works
Role of cyclooxygenase-2 in gastric cancer development and progression
Cheng, J. (2013) · World Journal of Gastroenterology · doi:10.3748/wjg.v19.i42.7361 · Cited by 101 · see citing works
Use of glucosamine and chondroitin in relation to mortality
Bell, G. A., Kantor, E. D., Lampe, J. W., Shen, D. D., & White, E. (2012) · European Journal of Epidemiology · doi:10.1007/s10654-012-9714-6 · Cited by 98 · see citing works
Immune-enhancing activities of chondroitin sulfate in murine macrophage RAW 264.7 cells
Wu, F., Zhou, C., Zhou, D., Ou, S., Liu, Z., & Huang, H. (2018) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2018.06.071 · Cited by 98 · see citing works
Association Between Use of Specialty Dietary Supplements and C-Reactive Protein Concentrations
Kantor, E. D., Lampe, J. W., Vaughan, T. L., Peters, U., Rehm, C. D., & White, E. (2012) · American Journal of Epidemiology · doi:10.1093/aje/kws186 · Cited by 82 · see citing works
Kantor, E. D., Lampe, J. W., Navarro, S. L., Song, X., Milne, G. L., & White, E. (2014) · The Journal of Alternative and Complementary Medicine · doi:10.1089/acm.2013.0323 · Cited by 60 · see citing works
Cheng, Z., & Li, L. (2016) · International Immunopharmacology · doi:10.1016/j.intimp.2016.02.011 · Cited by 43 · see citing works
Genetic variant of cyclooxygenase-2 in gastric cancer: More inflammation and susceptibility
Ji, X.-K., Madhurapantula, S. V., He, G., Wang, K., Song, C., Zhang, J., & Wang, K.-J. (2021) · World Journal of Gastroenterology · doi:10.3748/wjg.v27.i28.4653 · Cited by 37 · see citing works
Impact of health and lifestyle food supplements on periodontal tissues and health
Spahr, A., & Divnic‐Resnik, T. (2022) · Periodontology 2000 · doi:10.1111/prd.12455 · Cited by 27 · see citing works
Ejarque, R. A., Payá, M., Montesinos, M. C., Úbeda, A., Navalón, P., Herrero, M., Vergés, J., & Terencio, M. C. (2012) · Pharmacological Research · doi:10.1016/j.phrs.2012.12.004 · Cited by 24 · see citing works
Drug Screening Implicates Chondroitin Sulfate as a Potential Longevity Pill
Ewald, C. Y. (2021) · Frontiers in Aging · doi:10.3389/fragi.2021.741843 · Cited by 23 · see citing works
Specialty Supplement Use and Biologic Measures of Oxidative Stress and DNA Damage
Kantor, E. D., Ulrich, C. M., Owen, R. W., Schmezer, P., Neuhouser, M. L., Lampe, J. W., Peters, U., Shen, D. D., Vaughan, T. L., & White, E. (2013) · Cancer Epidemiology Biomarkers & Prevention · doi:10.1158/1055-9965.epi-13-0470 · Cited by 16 · see citing works
Enzymatic Production of Chondroitin Oligosaccharides and Its Sulfate Derivatives
Zhang, W., Xu, R., Jin, X., Wang, Y., Hu, L., Zhang, T., Du, G., & Kang, Z. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.951740 · Cited by 14 · see citing works
Nutraceutical Functionalities of Polysaccharides from Marine Invertebrates
Choi, B.-D., & Choi, Y. J. (2012) · Advances in food and nutrition research · doi:10.1016/b978-0-12-416003-3.00002-0 · Cited by 14 · see citing works
Kozlowski, E. O., Gomes, A. M., Silva, C. S., Pereira, M. S., Silva, A. C. E. S. D. V., & Pavão, M. S. G. (2011) · Springer eBooks · doi:10.1007/978-3-642-16833-8_7 · Cited by 11 · see citing works
A Sustainable Future: Fish Processing Byproducts
Bechtel, P. J., & Smiley, S. T. (2010) · doi:10.4027/sffpb.2010 · Cited by 7 · see citing works
Ascidian Tunicate Extracts Attenuate Rheumatoid Arthritis in a Collagen-induced Murine Model
Hong, S., Kwon, J., Lee, J., Lee, S., Lee, A. Y., Cho, W.-Y., Bat‐Erdene, M., Choi, B.-D., & Cho, M. (2014) · Natural Product Communications · doi:10.1177/1934578x1400900632 · Cited by 4 · see citing works
Tunicates: A Vertebrate Ancestral Source of Antitumor Compounds
Cooper, E. L., & Albert, R. (2014) · Springer eBooks · doi:10.1007/978-3-319-07145-9_18 · Cited by 4 · see citing works
Zhang, P., Tan, R., Huang, C., Guan, W., Jiang, G., Liang, C., Jiang, C., Zhang, J., Xu, Y., & Zhang, L. (2026) · Journal of Controlled Release · doi:10.1016/j.jconrel.2026.114907 · Cited by 3 · see citing works
Kornprobst, J. (2014) · Wiley eBooks · doi:10.1002/9783527335855.marprod028 · Cited by 2 · see citing works
Extraction and Determination of Chondroitin Sulfate from Fish Processing Byproducts
Stine, J. J., Wu, T., de Oliveira, A. C. M., Smiley, S., & Bechtel, P. J. (2010) · A Sustainable Future: Fish Processing Byproducts · doi:10.4027/sffpb.2010.04 · Cited by 1 · see citing works
Kornprobst, J. (2014) · Wiley eBooks · doi:10.1002/9783527335855.marprod282
Functional Properties of Ascidians By-products: Nutritional and Medicinal Value
Ryu, B., & Kim, S. (2013) · Springer eBooks · doi:10.1007/978-1-4614-9590-1_27
- Authors
- Xu, C.-X., Jin, H., Chung, Y.-S., Shin, J.-Y., Lee, K.-H., Beck, G. R., Palmos, G. N., Choi, B.-D., & Cho, M.-H.
- Affiliations
- Seoul National University (KR)
- Laboratory of Toxicology, College of Veterinary Medicine, and Nano Systems Institute, National Core Research Center, Seoul National University, Seoul 151-742, Korea; Laboratory of Molecular Oncology, Korea Institute of Radiological and Medical Sciences, Seoul 139-240, Korea; Division of Endocrinology, Metabolism and Lipids, Emory University School of Medicine, Atlanta, Georgia 30322; Division of Marine Life Science/Institute of Marine Industry, Gyeongsang National University, Tongyoung 650-160, Korea;...
- Corresponding author
- Choi, Byeong-Dae
- Cho, Myung-Haing
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-09-19
- Volume
- 56
- Issue
- 20
- Pages
- 9667-9675
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2041356412
- Cited by
- 24
- References
- 30
- Citations by year
- 2026: 1 · 2022: 2 · 2021: 2 · 2018: 1 · 2016: 1 · 2014: 5 · 2013: 4 · 2012: 4
- FWCI
- 0.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Seoul National University Open Repository (Seoul National University) (submittedVersion)
- Funders
- National Cancer Institute (CA 84573, K22CA084573)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Health Sciences › Medicine › Pharmacology › Inflammatory mediators and NSAID effects
- Keywords
- chondroitin sulfate, anti-inflammatory activity, NF-κB activation, VCAM-1, cyclooxygenase-2, phorbol myristate acetate, skin inflammation, edema, Akt signaling, cancer prevention
- MeSH terms
- Animals, Carcinogens, Cell Extracts, Chondroitin Sulfates, Female, Humans, Inflammation, Mice, Inbred ICR, Phorbol Esters, Phosphorylation, Random Allocation, Skin, Urochordata, Signal Transduction, Down-Regulation, Gene Expression, NF-kappa B, Vascular Cell Adhesion Molecule-1, Mice, Cyclooxygenase 2
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