The DOI matches its Crossref record.
Real-Time Quantitative PCR Detection of Genetically Modified Maximizer Maize and Roundup Ready Soybean in Some Representative Foods
Abstract
A fast and quantitative method was developed to detect transgenic "Maximizer" maize "event 176" (Novartis) and "Roundup Ready" soybean (Monsanto) in food by real-time quantitative PCR. The use of the ABI Prism 7700 sequence detection system allowed the determination of the amplified product accumulation through a fluorogenic probe (TaqMan). Fluorescent dyes were chosen in such a way as to coamplify total and transgenic DNA in the same tube. Using real-time quantitative PCR, 2 pg of transgenic or total DNA per gram of starting sample was detected in 3 h after DNA extraction and the relative amounts of "Maximizer" maize and "Roundup Ready" soybean in some representative food products were quantified.
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Citations
Cited by
Real-time PCR: what relevance to plant studies?
Gachon, C., Mingam, A., & Charrier, B. (2004) · Journal of Experimental Botany · doi:10.1093/jxb/erh181 · Cited by 454
Demeke, T., & Jenkins, G. R. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-009-3150-9 · Cited by 403
Detection of genetically modified organisms in foods
Ahmed, F. E. (2002) · Trends in Biotechnology · doi:10.1016/s0167-7799(01)01920-5 · Cited by 373
Food authentication by PCR-based methods
Mafra, I., Ferreira, I. M. P. L. V. O., & Oliveira, M. B. P. P. (2008) · European Food Research and Technology · doi:10.1007/s00217-007-0782-x · Cited by 372
García-Villalba, R., León, C., Dinelli, G., Segura-Carretero, A., Fernández-Gutiérrez, A., Garcia-Cañas, V., & Cifuentes, A. (2008) · Journal of Chromatography A · doi:10.1016/j.chroma.2008.05.018 · Cited by 129
Metadata
- Authors
- Vaïtilingom, M., Pijnenburg, H., Gendre, F., & Brignon, P.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-11-24
- Volume
- 47
- Issue
- 12
- Pages
- 5261-5266
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1980227479
- Cited by
- 217
- References
- 0
- FWCI
- 10.59
- Type
- journal article
- Language
- en
- Open access
- closed
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- Vaïtilingom, M., Pijnenburg, H., Gendre, F., & Brignon, P. (1999). Real-Time Quantitative PCR Detection of Genetically Modified Maximizer Maize and Roundup Ready Soybean in Some Representative Foods. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf981208v
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
