The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.
Real-time PCR: what relevance to plant studies?
Abstract
The appearance of genetically modified organisms on the food market a few years ago, and the demand for more precise and reliable techniques to detect foreign (transgenic or pathogenic) DNA in edible plants, have been the driving force for the introduction of real-time PCR techniques in plant research. This was followed by numerous fundamental research applications aiming to study the expression profiles of endogenous genes and multigene families. Since then, the interest in this technique in the plant scientist community has increased exponentially. This review describes the technical features of quantitative real-time PCR that are especially relevant to plant research, and summarizes its present and future applications.
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Citations
Cites
A new mathematical model for relative quantification in real-time RT-PCR
Pfaffl, M. W. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.9.e45 · Cited by 34,610
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
Initiative, T. A. G. (2000) · Nature · doi:10.1038/35048692 · Cited by 9,301
Heid, C. A., Stevens, J., Livak, K. J., & Williams, P. M. (1996) · Genome Research · doi:10.1101/gr.6.10.986 · Cited by 6,174
Bustin, S. (2000) · Journal of Molecular Endocrinology · doi:10.1677/jme.0.0250169 · Cited by 3,872
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems
Bustin, S. (2002) · Journal of Molecular Endocrinology · doi:10.1677/jme.0.0290023 · Cited by 2,546
Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions
Higuchi, R., Fockler, C., Dollinger, G., & Watson, R. (1993) · Nature Biotechnology · doi:10.1038/nbt0993-1026 · Cited by 2,159
Continuous Fluorescence Monitoring of Rapid Cycle DNA Amplification
Wittwer, C. T., Herrmann, M. G., Moss, A. A., & Rasmussen, R. P. (2013) · BioTechniques · doi:10.2144/000114043 · Cited by 1,274
Quantitative RT-PCR: Pitfalls and Potential
Freeman, W. M., Walker, S. J., & Vrana, K. E. (1999) · BioTechniques · doi:10.2144/99261rv01 · Cited by 1,147
Continuous Fluorescence Monitoring of Rapid Cycle DNA Amplification
Wittwer, C. T., Herrmann, M. G., Moss, A. A., & Rasmussen, R. P. (1997) · BioTechniques · doi:10.2144/97221bi01 · Cited by 1,143
The LightCycler TM : A Microvolume Multisample Fluorimeter with Rapid Temperature Control
Wittwer, C. T., Ririe, K. M., Andrew, R. V., David, D. A., Gundry, R. A., & Balis, U. J. (1997) · BioTechniques · doi:10.2144/97221pf02 · Cited by 893
Cited by
Czechowski, T., Stitt, M., Altmann, T., Udvardi, M. K., & Scheible, W.-R. (2005) · Plant Physiology · doi:10.1104/pp.105.063743 · Cited by 3,203
Expósito-Rodríguez, M., Borges, A. A., Borges-Pérez, A., & Pérez, J. A. (2008) · BMC Plant Biology · doi:10.1186/1471-2229-8-131 · Cited by 787
Gutierrez, L., Mauriat, M., Guénin, S., Pelloux, J., Lefebvre, J., Louvet, R., Rusterucci, C., Moritz, T., Guerineau, F., Bellini, C., et al. (2008) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2008.00346.x · Cited by 696
Identification and validation of reference genes for quantitative RT-PCR normalization in wheat
Paolacci, A. R., Tanzarella, O. A., Porceddu, E., & Ciaffi, M. (2009) · BMC Molecular Biology · doi:10.1186/1471-2199-10-11 · Cited by 687
Schmidt, G. W., & Delaney, S. K. (2010) · Molecular Genetics and Genomics · doi:10.1007/s00438-010-0511-1 · Cited by 553
Metadata
- Authors
- Gachon, C., Mingam, A., & Charrier, B.
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-06-18
- Volume
- 55
- Issue
- 402
- Pages
- 1445-1454
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- OpenAlex
- W2111464410
- Cited by
- 454
- References
- 65
- FWCI
- 5.47
- Type
- journal article
- Language
- en
- Open access
- closed
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