Cited by 129Nitric Oxide and Endothelin EffectsFree Radicals and AntioxidantsRedox biology and oxidative stress

Sodium Nitroprusside: Mechanism of NO Release Mediated by Sulfhydryl-Containing Molecules

Grossi, Loris, D'Angelo, Sandra·2005·Journal of Medicinal Chemistry

Abstract

Sodium nitroprusside (SNP) is among the most widely studied nitric oxide donors, and its capability of producing NO seems to depend on its interaction with sulfhydryl-containing molecules present in vivo. The aim of this research has been the study of the mechanism of interaction between SNP and sulfhydryl-containing compounds, such as cysteine and glutathione, through detection by EPR, UV-vis, and IR spectroscopy of both the radical and nonradical species involved. An electron-transfer process can be invoked as the key step, which leads to the formation of the reduced SNP radical, the main detectable radical intermediate, and the corresponding S-nitrosothiol, the ending product of NO that can be considered the real storage and transporters of NO. When cysteine was used, a second radical species (A) is detectable: it can be accounted for by the interaction of a byproduct with unreacted cysteine.

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Crossref · 10.1021/jm049857n

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Authors
Grossi, L., & D'Angelo, S.
Affiliations
  • University of Bologna (IT)
  • Dipartimento di Chimica Organica “A. Mangini”, Università di Bologna, Viale Risorgimento 4, I-40136 Bologna, Italy
Corresponding author
Grossi, Loris
Venue
Journal of Medicinal Chemistry
Published
2005-02-23
Volume
48
Issue
7
Pages
2622-2626
ISSN
  • 0022-2623 (ISSN-L)
  • 1520-4804
OpenAlex
W2011675072
Cited by
129
References
25
Citations by year
2026: 3 · 2025: 5 · 2024: 9 · 2023: 8 · 2022: 13 · 2021: 7 · 2020: 3 · 2019: 12 · 2018: 11 · 2017: 10 · 2016: 5 · 2015: 5 · 2014: 3 · 2013: 7 · 2012: 3
FWCI
2.40
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Medicinal Chemistry (publishedVersion)
  • PubMed (publishedVersion)
  • Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
sodium nitroprusside, nitric oxide donors, nitric oxide release, cysteine, glutathione, electron transfer, S-nitrosothiols, radical intermediates
MeSH terms
Buffers, Electron Spin Resonance Spectroscopy, Hydrogen-Ion Concentration, Nitric Oxide, Nitroprusside, Phosphates, Spectrophotometry, Infrared, Spectrophotometry, Ultraviolet, Water, Nitric Oxide Donors

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