Sodium Nitroprusside: Mechanism of NO Release Mediated by Sulfhydryl-Containing Molecules
Abstract
Sodium nitroprusside (SNP) is among the most widely studied nitric oxide donors, and its capability of producing NO seems to depend on its interaction with sulfhydryl-containing molecules present in vivo. The aim of this research has been the study of the mechanism of interaction between SNP and sulfhydryl-containing compounds, such as cysteine and glutathione, through detection by EPR, UV-vis, and IR spectroscopy of both the radical and nonradical species involved. An electron-transfer process can be invoked as the key step, which leads to the formation of the reduced SNP radical, the main detectable radical intermediate, and the corresponding S-nitrosothiol, the ending product of NO that can be considered the real storage and transporters of NO. When cysteine was used, a second radical species (A) is detectable: it can be accounted for by the interaction of a byproduct with unreacted cysteine.
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Self-citation
- Authors
- Grossi, L., & D'Angelo, S.
- Affiliations
- University of Bologna (IT)
- Dipartimento di Chimica Organica “A. Mangini”, Università di Bologna, Viale Risorgimento 4, I-40136 Bologna, Italy
- Corresponding author
- Grossi, Loris
- ORCID
- Grossi, Loris
- Venue
- Journal of Medicinal Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-02-23
- Volume
- 48
- Issue
- 7
- Pages
- 2622-2626
- ISSN
- 0022-2623 (ISSN-L)
- 1520-4804
- OpenAlex
- W2011675072
- Cited by
- 129
- References
- 25
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 9 · 2023: 8 · 2022: 13 · 2021: 7 · 2020: 3 · 2019: 12 · 2018: 11 · 2017: 10 · 2016: 5 · 2015: 5 · 2014: 3 · 2013: 7 · 2012: 3
- FWCI
- 2.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Medicinal Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- sodium nitroprusside, nitric oxide donors, nitric oxide release, cysteine, glutathione, electron transfer, S-nitrosothiols, radical intermediates
- MeSH terms
- Buffers, Electron Spin Resonance Spectroscopy, Hydrogen-Ion Concentration, Nitric Oxide, Nitroprusside, Phosphates, Spectrophotometry, Infrared, Spectrophotometry, Ultraviolet, Water, Nitric Oxide Donors
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