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Cited by 494

The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.

Biological Water: Femtosecond Dynamics of Macromolecular Hydration

Open access
Pal, Samir Kumar, Peon, Jorge, Bagchi, Biman, Zewail, Ahmed H.·2002·The Journal of Physical Chemistry B
DOI 10.1021/jp0213506
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Abstract

The unique features of a macromolecule and water as a solvent make the issue of solvation unconventional, with questions about the static versus dynamic nature of hydration and the physics of orientational and translational diffusion at the boundary. For proteins, the hydration shell that covers the surface is critical to the stability of its structure and function. Dynamically speaking, the residence time of water at the surface is a signature of its mobility and binding. With femtosecond time resolution it is possible to unravel the shortest residence times which are key for the description of the hydration layer, static or dynamic. In this article we review these issues guided by experimental studies, from this laboratory, of polar hydration dynamics at the surfaces of two proteins (Subtilisin Carlsberg (SC) and Monellin). The natural probe tryptophan amino acid was used for the interrogation of the dynamics, and for direct comparison we also studied the behavior in bulk water a complete hydration in 1 ps. We develop a theoretical description of solvation and relate the theory to the experimental observations. In this theoretical approach, we consider the dynamical equilibrium in the hydration shell, defining the rate processes for breaking and making the transient hydrogen bonds, and the effective friction in the layer which is defined by the translational and orientational motions of water molecules. The relationship between the residence time of water molecules and the observed slow component in solvation dynamics is a direct one. For the two proteins studied, we observed a “bimodal decay” for the hydration correlation function, with two primary relaxation times: ultrafast, typically 1 ps or less, and longer, typically 15−40 ps, and both are related to the residence time at the protein surface, depending on the binding energies. We end by making extensions to studies of the denatured state of the protein, random coils, and the biomimetic micelles, and conclude with our thoughts on the relevance of the dynamics of native structures to their functions.

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Metadata

Authors
Pal, S. K., Peon, J., Bagchi, B., & Zewail, A. H.
Venue
The Journal of Physical Chemistry B
Publisher
American Chemical Society (ACS)
Published
2002-11-07
Volume
106
Issue
48
Pages
12376-12395
ISSN
  • 1520-5207 (ISSN-L)
  • 1520-6106
DOI
10.1021/jp0213506
OpenAlex
W2057742133
Cited by
494
References
34
FWCI
16.34
Type
journal article
Language
en
Open access
bronze

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