The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Observation of Individual Microtubule Motor Steps in Living Cells with Endocytosed Quantum Dots
Abstract
We report the observation of individual steps taken by motor proteins in living cells by following movements of endocytic vesicles that contain quantum dots (QDs) with a fast camera. The brightness and photostability of quantum dots allow us to record motor displacement traces with 300 micros time resolution and 1.5 nm spatial precision. We observed individual 8 nm steps in active transport toward both the microtubule plus- and minus-ends, the directions of kinesin and dynein movements, respectively. In addition, we clearly resolved abrupt 16 nm steps in the plus-end direction and often consecutive 16 nm and occasional 24 nm steps in minus-end directed movements. This work demonstrates the ability of the QD assay to probe the operation of motor proteins at the molecular level in living cells under physiological conditions.
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Cites
Mechanics of Motor Proteins and the Cytoskeleton
Howard, & Clark (2002) · Applied Mechanics Reviews · doi:10.1115/1.1451234 · Cited by 2,909
Precise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Bead movement by single kinesin molecules studied with optical tweezers
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Force production by single kinesin motors
Schnitzer, M. J., Visscher, K., & Block, S. M. (2000) · Nature Cell Biology · doi:10.1038/35036345 · Cited by 572
Coupling of kinesin steps to ATP hydrolysis
Hua, W., Young, E. C., Fleming, M. L., & Gelles, J. (1997) · Nature · doi:10.1038/41118 · Cited by 358
The kinetic cycles of myosin, kinesin, and dynein
Hackney, D. D. (1996) · Annual Review of Physiology · doi:10.1146/annurev.ph.58.030196.003503 · Cited by 118
Cited by
Multicolor Super-Resolution Imaging with Photo-Switchable Fluorescent Probes
Bates, M., Huang, B., Dempsey, G. T., & Zhuang, X. (2007) · Science · doi:10.1126/science.1146598 · Cited by 1,504
A cell nanoinjector based on carbon nanotubes
Chen, X., Kis, A., Zettl, A., & Bertozzi, C. R. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0700567104 · Cited by 377
Processive bidirectional motion of dynein–dynactin complexes in vitro
Ross, J. L., Wallace, K., Shuman, H., Goldman, Y. E., & Holzbaur, E. L. F. (2006) · Nature Cell Biology · doi:10.1038/ncb1421 · Cited by 288
Unambiguous observation of shape effects on cellular fate of nanoparticles
Chu, Z., Zhang, S., Zhang, B., Zhang, C., Fang, C.-Y., Rehor, I., Cigler, P., Chang, H.-C., Lin, G., Liu, R., et al. (2014) · Scientific Reports · doi:10.1038/srep04495 · Cited by 277
Xie, X. S., Yu, J., & Yang, W. Y. (2006) · Science · doi:10.1126/science.1127566 · Cited by 257
Self-citation
Metadata
- Authors
- Nan, X., Sims, P. A., Chen, P., & Xie, X. S.
- Venue
- The Journal of Physical Chemistry B
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-12-01
- Volume
- 109
- Issue
- 51
- Pages
- 24220-24224
- ISSN
- 1520-5207 (ISSN-L)
- 1520-6106
- OpenAlex
- W2168002602
- Cited by
- 167
- References
- 7
- FWCI
- 7.42
- Type
- journal article
- Language
- en
- Open access
- closed
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- Nan, X., Sims, P. A., Chen, P., & Xie, X. S. (2005). Observation of Individual Microtubule Motor Steps in Living Cells with Endocytosed Quantum Dots. The Journal of Physical Chemistry B. https://doi.org/10.1021/jp056360w
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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