The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Living Cells as Test Tubes
Abstract
The combination of specific probes and advanced optical microscopy now allows quantitative probing of biochemical reactions in living cells. On selected systems, one can detect and track a particular protein with single-molecule sensitivity, nanometer spatial precision, and millisecond time resolution. Metabolites, usually difficult to detect, can be imaged and monitored in living cells with coherent anti-Stokes Raman scattering microscopy. Here, we describe the application of these techniques in studying gene expression, active transport, and lipid metabolism.
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Cites
Two-photon laser scanning fluorescence microscopy
Denk, W., Strickler, J. H., & Webb, W. W. (1990) · Science · doi:10.1126/science.2321027 · Cited by 9,488
Semiconductor Nanocrystals as Fluorescent Biological Labels
Bruchez, M., Moronne, M., Gin, P., Weiss, S., & Alivisatos, A. P. (1998) · Science · doi:10.1126/science.281.5385.2013 · Cited by 8,613
Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection
Chan, W. C. W., & Nie, S. (1998) · Science · doi:10.1126/science.281.5385.2016 · Cited by 7,278
A guide to choosing fluorescent proteins
Shaner, N. C., Steinbach, P. A., & Tsien, R. Y. (2005) · Nature Methods · doi:10.1038/nmeth819 · Cited by 2,840
Precise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Direct observation of kinesin stepping by optical trapping interferometry
Svoboda, K., Schmidt, C. F., Schnapp, B. J., & Block, S. M. (1993) · Nature · doi:10.1038/365721a0 · Cited by 1,910
Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization
Yildiz, A., Forkey, J. N., McKinney, S. A., Ha, T., Goldman, Y. E., & Selvin, P. R. (2003) · Science · doi:10.1126/science.1084398 · Cited by 1,837
Noise in Gene Expression: Origins, Consequences, and Control
Raser, J. M., & O'Shea, E. K. (2005) · Science · doi:10.1126/science.1105891 · Cited by 1,750
Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering
Zumbusch, A., Holtom, G. R., & Xie, X. S. (1999) · Physical Review Letters · doi:10.1103/physrevlett.82.4142 · Cited by 1,626
Self-citationReal-Time Kinetics of Gene Activity in Individual Bacteria
Golding, I., Paulsson, J., Zawilski, S. M., & Cox, E. C. (2005) · Cell · doi:10.1016/j.cell.2005.09.031 · Cited by 1,468
Cited by
Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine
Cheng, J.-X., & Xie, X. S. (2015) · Science · doi:10.1126/science.aaa8870 · Cited by 874
Self-citationAlkyne-Tag Raman Imaging for Visualization of Mobile Small Molecules in Live Cells
Yamakoshi, H., Dodo, K., Palonpon, A., Ando, J., Fujita, K., Kawata, S., & Sodeoka, M. (2012) · Journal of the American Chemical Society · doi:10.1021/ja308529n · Cited by 461
Micro‐Raman spectroscopy of algae: Composition analysis and fluorescence background behavior
Huang, Y. Y., Beal, C. M., Cai, W. W., Ruoff, R. S., & Terentjev, E. M. (2010) · Biotechnology and Bioengineering · doi:10.1002/bit.22617 · Cited by 145
Orban, T., Palczewska, G., & Palczewski, K. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.195198 · Cited by 89
Metadata
- Authors
- Xie, X. S., Yu, J., & Yang, W. Y.
- Venue
- Science
- Published
- 2006-04-14
- Volume
- 312
- Issue
- 5771
- Pages
- 228-230
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2138857126
- Cited by
- 257
- References
- 29
- FWCI
- 17.83
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
