The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Generation of Solution and Surface Gradients Using Microfluidic Systems
Abstract
This paper describes a simple, versatile method of generating gradients in composition in solution or on surfaces using microfluidic systems. This method is based on controlled diffusive mixing of species in solutions that are flowing laminarly, at low Reynolds number, inside a network of microchannels. We demonstrate the use of this procedure to generate (1) gradients in the compositions of solutions, measured directly by colorimetric assays and (2) gradients in topography of the surfaces produced by generating concentration gradients of etching reagents, and then using these gradients to etch profiles into the substrate. The lateral dimensions of the gradients examined here, which went from 350 to 900 μm, are determined by the width of the microchannels. Gradients of different size, resolution, and shape have been generated using this method. The shape of the gradients can be changed continuously (dynamic gradients) by varying the relative flow velocities of the input streams of fluids. The method is experimentally simple and highly adaptable, and requires no special equipment except for an elastomeric relief structure that can be readily prepared by rapid prototyping. This technique provides a new platform with which to study phenomena that depend on gradients in concentration, especially dynamic phenomena in cell biology (chemotaxis and haptotaxis) and surface chemistry (nucleation and growth of crystals, etching, and Marangoni effects).
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Cites
Park, G. S. (1975) · Polymer · doi:10.1016/0032-3861(75)90130-5 · Cited by 14,967
Notch Signaling: Cell Fate Control and Signal Integration in Development
Artavanis-Tsakonas, S., Rand, M. D., & Lake, R. J. (1999) · Science · doi:10.1126/science.284.5415.770 · Cited by 5,909
Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane)
Duffy, D. C., McDonald, J. C., Schueller, O. J. A., & Whitesides, G. M. (1998) · Analytical Chemistry · doi:10.1021/ac980656z · Cited by 5,288
Self-citationParent, C. A., & Devreotes, P. N. (1999) · Science · doi:10.1126/science.284.5415.765 · Cited by 890
Control of crystal nucleation by patterned self-assembled monolayers
Aizenberg, J., Black, A. J., & Whitesides, G. M. (1999) · Nature · doi:10.1038/19047 · Cited by 853
Self-citationAizenberg, J., Black, A. J., & Whitesides, G. M. (1999) · Journal of the American Chemical Society · doi:10.1021/ja984254k · Cited by 491
Self-citationComplete asymmetric induction of supramolecular chirality in a hydrogen-bonded assembly
Prins, L. J., Huskens, J., de Jong, F., Timmerman, P., & Reinhoudt, D. N. (1999) · Nature · doi:10.1038/19053 · Cited by 481
Spatial control of actin polymerization during neutrophil chemotaxis
Weiner, O. D., Servant, G., Welch, M. D., Mitchison, T. J., Sedat, J. W., & Bourne, H. R. (1999) · Nature Cell Biology · doi:10.1038/10042 · Cited by 284
Molecular Gradients of .omega.-Substituted Alkanethiols on Gold: Preparation and Characterization
Liedberg, B., & Tengvall, P. (1995) · Langmuir · doi:10.1021/la00010a037 · Cited by 173
Terrill, R. H., Balss, K. M., Zhang, Y., & Bohn, P. W. (2000) · Journal of the American Chemical Society · doi:10.1021/ja993442n · Cited by 130
Bacterial chemotaxis: The five sensors of a bacterium
Grebe, T. W., & Stock, J. (1998) · Current Biology · doi:10.1016/s0960-9822(98)00098-0 · Cited by 124
Assays of leukocyte locomotion and chemotaxis
Wilkinson, P. C. (1998) · Journal of Immunological Methods · doi:10.1016/s0022-1759(98)00075-1 · Cited by 107
Formation of Microscale Gradients of Protein Using Heterobifunctional Photolinkers
Hypolite, C. L., McLernon, T. L., Adams, D. N., et al. (1997) · Bioconjugate Chemistry · doi:10.1021/bc9701252 · Cited by 90
Cited by
The present and future role of microfluidics in biomedical research
Sackmann, E. K., Fulton, A. L., & Beebe, D. J. (2014) · Nature · doi:10.1038/nature13118 · Cited by 2,995
Direct Ink Writing of 3D Functional Materials
Lewis, J. A. (2006) · Advanced Functional Materials · doi:10.1002/adfm.200600434 · Cited by 1,740
Microfabrication meets microbiology
Weibel, D. B., DiLuzio, W. R., & Whitesides, G. M. (2007) · Nature Reviews Microbiology · doi:10.1038/nrmicro1616 · Cited by 788
Self-citationBiomolecular gradients in cell culture systems
Keenan, T. M., & Folch, A. (2008) · Lab Chip · doi:10.1039/b711887b · Cited by 596
Gradient biomaterials for soft-to-hard interface tissue engineering
Seidi, A., Ramalingam, M., Elloumi-Hannachi, I., Ostrovidov, S., & Khademhosseini, A. (2011) · Acta Biomaterialia · doi:10.1016/j.actbio.2011.01.011 · Cited by 398
Metadata
- Authors
- Jeon, N. L., Dertinger, S. K. W., Chiu, D. T., Choi, I. S., Stroock, A. D., & Whitesides, G. M.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-10-01
- Volume
- 16
- Issue
- 22
- Pages
- 8311-8316
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W1969435013
- Cited by
- 963
- References
- 13
- FWCI
- 12.80
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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