Cell and protein compatibility of parylene-C surfaces
Abstract
Parylene-C, which is traditionally used to coat implantable devices, has emerged as a promising material to generate miniaturized devices due to its unique mechanical properties and inertness. In this paper we compared the surface properties and cell and protein compatibility of parylene-C relative to other commonly used BioMEMS materials. We evaluated the surface hydrophobicity and roughness of parylene-C and compared these results to those of tissue culture-treated polystyrene, poly(dimethylsiloxane) (PDMS), and glass. We also treated parylene-C and PDMS with air plasma, and coated the surfaces with fibronectin to demonstrate that biochemical treatments modify the surface properties of parylene-C. Although plasma treatment caused both parylene-C and PDMS to become hydrophilic, only parylene-C substrates retained their hydrophilic properties over time. Furthermore, parylene-C substrates display a higher degree of nanoscale surface roughness (>20 nm) than the other substrates. We also examined the level of BSA and IgG protein adsorption on various surfaces and found that surface plasma treatment decreased the degree of protein adsorption on both PDMS and parylene-C substrates. After testing the degree of cell adhesion and spreading of two mammalian cell types, NIH-3T3 fibroblasts and AML-12 hepatocytes, we found that the adhesion of both cell types to surface-treated parylene-C variants were comparable to standard tissue culture substrates, such as polystyrene. Overall, these results indicate that parylene-C, along with its surface-treated variants, could potentially be a useful material for fabricating cell-based microdevices.
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Parylene C has a well-established history of biocompatibility and biostability.
“Parylene C has recently arisen as a good material option for peripheral nerve devices (de la Oliva et al., 2018), and has a well-established history of biocompatibility and biostability (Chang et al., 2007; Winslow et al., 2010; Hassler et al., 2011).”
From Foreign Body Reaction to Implanted Biomaterials and Its Impact in Nerve Neuroprosthetics (2021) · cites this paper as Chang et al., 2007
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“Parylene C has recently arisen as a good material option for peripheral nerve devices (de la Oliva et al., 2018), and has a wellestablished history of biocompatibility and biostability (Chang et al., 2007; Winslow et al., 2010; Hassler et al., 2011).”
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- Authors
- Chang, T. Y., Yadav, V. G., De Leo, S., Mohedas, A., Rajalingam, B., Chen, C.-L., Selvarasah, S., Dokmeci, M. R., & Khademhosseini, A.
- Affiliations
- Louisiana State University (US)
- Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, Department of Chemical Engineering, University of Waterloo, Waterloo, Canada N2L 3G1, Department of Biological Engineering, Louisiana State University, Baton Rouge, Louisiana 70808, Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02139,...
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-10-04
- Volume
- 23
- Issue
- 23
- Pages
- 11718-11725
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2046039187
- Cited by
- 320
- References
- 41
- Citations by year
- 2026: 6 · 2025: 14 · 2024: 12 · 2023: 10 · 2022: 7 · 2021: 13 · 2020: 17 · 2019: 18 · 2018: 15 · 2017: 23 · 2016: 33 · 2015: 21 · 2014: 20 · 2013: 25 · 2012: 22
- FWCI
- 7.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Langmuir (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- Parylene C, BioMEMS, surface hydrophobicity, surface roughness, air plasma treatment, fibronectin coating, protein adsorption, BSA, IgG, cell adhesion, NIH3T3 cells, polydimethylsiloxane
- MeSH terms
- Animals, Cell Adhesion, Cell Line, Dimethylpolysiloxanes, Fibroblasts, Glass, Immunoglobulin G, Polymers, Polystyrenes, Proteins, Serum Albumin, Bovine, Silicones, Surface Properties, Xylenes, 3T3 Cells, Fluorescein-5-isothiocyanate, Microscopy, Atomic Force, Coated Materials, Biocompatible, Hepatocytes, Tissue Engineering, Mice, Hydrophobic and Hydrophilic Interactions
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