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In Situ Assembly of Colloidal Particles into Miniaturized Biosensors
Abstract
We show how to create arrays of biosensors by in situ assembly of colloidal particles onto micropatterned electrodes. Latex microspheres from suspension are collected via dielectrophoresis in the micrometer-sized gaps between planar electrodes. The assembled particulate patches are fixed by changing the colloidal interactions to induce coagulation. Immuno-active sites on the latex surfaces bind the target molecules. A direct electric conductivity readout is accomplished after secondary tagging with colloidal gold and its enhancement by silver nucleation. The method holds promise for creating disposable on-chip arrays of highly sensitive miniature sensors for specific proteins, DNA fragments, or other biomolecules.
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Cited by
Applications of Nanoparticles in Biology
De, M., Ghosh, P. S., & Rotello, V. M. (2008) · Advanced Materials · doi:10.1002/adma.200703183 · Cited by 1,547
Dielectrophoretic Assembly of Electrically Functional Microwires from Nanoparticle Suspensions
Hermanson, K. D., Lumsdon, S. O., Williams, J. P., Kaler, E. W., & Velev, O. D. (2001) · Science · doi:10.1126/science.1063821 · Cited by 561
Self-citationBaron, R., Zayats, M., & Willner, I. (2005) · Analytical Chemistry · doi:10.1021/ac048691v · Cited by 373
Amino-Functionalized Polystyrene Nanoparticles Activate the NLRP3 Inflammasome in Human Macrophages
Lunov, O., Syrovets, T., Loos, C., Nienhaus, G. U., Mailänder, V., Landfester, K., Rouis, M., & Simmet, T. (2011) · ACS Nano · doi:10.1021/nn203596e · Cited by 262
Metadata
- Authors
- Velev, O. D., & Kaler, E. W.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-04-30
- Volume
- 15
- Issue
- 11
- Pages
- 3693-3698
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2142091096
- Cited by
- 472
- References
- 0
- FWCI
- 11.21
- Type
- journal article
- Language
- en
- Open access
- closed
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- Velev, O. D., & Kaler, E. W. (1999). In Situ Assembly of Colloidal Particles into Miniaturized Biosensors. Langmuir. https://doi.org/10.1021/la981729c
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