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Cited by 254

The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.

Preparation of Mixed Self-Assembled Monolayers (SAMs) That Resist Adsorption of Proteins Using the Reaction of Amines with a SAM That Presents Interchain Carboxylic Anhydride Groups

Chapman, Robert G., Ostuni, Emanuele, Yan, Lin, Whitesides, George M.·2000·Langmuir
DOI 10.1021/la991579l
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Abstract

This paper describes a procedure for preparing mixed self-assembled monolayers (mixed SAMs) on gold that resist the nonspecific adsorption of proteins from solution. This method was tested using α-amino derivatives of ω-hydroxy- and ω-methoxy-oligo(ethylene glycols): H 2 N(CH 2 CH 2 O) n CH 3 and H 2 N(CH 2 CH 2 O) n H ( n = 3, 6). Mixed SAMs were prepared by allowing these amines to react with a SAM presenting interchain carboxylic anhydride groups. The resistance of the resulting surfaces to adsorption of several proteins carbonic anhydrase (EC 4.2.1.1), ribonuclease A (EC 3.1.27.5), lysozyme (EC 3.2.1.17), and fibrinogen was examined using surface plasmon resonance (SPR) spectroscopy. These mixed SAMs resist the nonspecific adsorption of proteins approximately as effectively as single-component SAMs prepared using the conventional method involving chemisorption of oligo(ethylene glycol)-terminated alkanethiols on gold. Characterization of the mixed SAM that presents a 1:1 mixture of −OCNH(CH 2 CH 2 O) 6 CH 3 and CO 2 H/CO 2 - groups by polarized infrared external reflectance spectroscopy indicates that the ethylene glycol units are in an amorphous conformation. A model surface for use in studies of biospecific adsorption was synthesized by reacting the anhydride groups with a mixture of H 2 N(CH 2 CH 2 O) 6 H and H 2 N(CH 2 CH 2 O) 6 CH 2 CONH(CH 2 ) 6 NHCOC 6 H 4 SO 2 NH 2; the resulting system was examined for its ability to bind bovine carbonic anhydrase by SPR. The values of the relevant constants were k off = 0.0054 s - 1, k on = 13 000 M - 1 s - 1, and K d = 0.42 μM. These values agree with values obtained by other means. The reaction of amines with SAMs that present interchain carboxylic anhydrides provides an experimentally simple route to the formation of mixed SAMs that resist the nonspecific adsorption of proteins or that adsorb a protein of interest biospecifically.

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Metadata

Authors
Chapman, R. G., Ostuni, E., Yan, L., & Whitesides, G. M.
Venue
Langmuir
Publisher
American Chemical Society (ACS)
Published
2000-07-25
Volume
16
Issue
17
Pages
6927-6936
ISSN
  • 0743-7463 (ISSN-L)
  • 1520-5827
DOI
10.1021/la991579l
OpenAlex
W2047762049
Cited by
254
References
21
FWCI
10.09
Type
journal article
Language
en
Open access
closed

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