Untangling the Effects of Peptide Sequences and Nanotopographies in a Biomimetic Niche for Directed Differentiation of iPSCs by Assemblies of Genetically Engineered Viral Nanofibers
Open accessAbstract
Here we report the design of a unique matrix, assembled from engineered M13 phage bionanofibers with specific cues of nanotopographies and versatile signal peptides to simulate native niche for directing the fate of induced pluripotent stem cells (iPSCs). By independently varying the peptide sequences and nanotopographies, we find that the resident iPSCs on the phage matrix are first differentiated into mesenchymal progenitor cells (MPCs), which are further differentiated into osteoblasts in the absence of osteogenic supplements due to the elongation induced by phage nanofibers. The phage-based matrix represents not only a biomimetic stem cell niche enabling independently varying biochemical and biophysical cues in one system but also a substrate for generating a safe and efficient cell source for tissue engineering.
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Implantation of seed cells such as hADSCs faces significant challenges including immune rejection, teratoma formation, and undirected cell differentiation.
“However, the implantation of seed cells, such as hADSCs, faces significant challenges, including immune rejection [6], teratoma formation [7] and undirected cell differentiation [8].”
From Exosome-functionalized magnesium-organic framework-based scaffolds with osteogenic, angiogenic and anti-inflammatory properties for accelerated bone regeneration. (2022) · cites this paper as [7]
Citation [8] is attached to undirected cell differentiation, not the separately cited immune-rejection and teratoma claims. The paper’s introductory passage (indexed under Abstract; page unavailable) says directing iPSCs toward osteoblastic differentiation is a challenge, but it does not establish undirected differentiation as a challenge when implanting hADSCs. (LLM, conf=0.83)
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- Authors
- Wang, J., Wang, L., Yang, M., Zhu, Y., Tomsia, A., & Mao, C.
- Affiliations
- University of Oklahoma (US)
- Zhejiang University (CN)
- Lawrence Berkeley National Laboratory (US)
- Corresponding author
- Yang, Mingying
- Mao, Chuanbin
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2014-12-02
- Volume
- 14
- Issue
- 12
- Pages
- 6850-6856
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- PMC
- PMC4291459
- OpenAlex
- W2333767959
- Cited by
- 90
- References
- 35
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 4 · 2023: 8 · 2022: 11 · 2021: 7 · 2020: 6 · 2019: 8 · 2018: 6 · 2017: 12 · 2016: 12 · 2015: 8
- FWCI
- 4.38
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nano Letters (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Biomedical Imaging and Bioengineering (R21EB015190)
- National Institute of Dental and Craniofacial Research (R01DE015633)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- induced pluripotent stem cells, nanotopography, peptide sequences, mesenchymal progenitor cells, osteogenic differentiation, cell elongation, bioactive scaffolds, tissue engineering
- MeSH terms
- Cell Differentiation, Cell Line, Extracellular Matrix, Genetic Engineering, Humans, Materials Testing, Osteoblasts, Osteogenesis, Particle Size, Surface Properties, Bacteriophage M13, Tissue Engineering, Biomimetic Materials, Stem Cell Niche, Induced Pluripotent Stem Cells, Nanofibers, Cell Surface Display Techniques
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