The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Localizing and Tracking Single Nanoscale Emitters in Three Dimensions with High Spatiotemporal Resolution Using a Double-Helix Point Spread Function
Open accessAbstract
Three-dimensional nanoscale localization and tracking of dim single emitters can be obtained with a widefield fluorescence microscope exhibiting a double-helix point spread function (DH-PSF). We describe in detail how the localization precision quantitatively depends upon the number of photons detected and the z position of the nanoscale emitter, thereby showing a approximately 10 nm localization capability along x, y, and z in the limit of weak emitters. Experimental measurements are compared to Fisher information calculations of the ultimate localization precision inherent in the DH-PSF. The DH-PSF, for the first time, is used to track single quantum dots in aqueous solution and a quantum dot-labeled structure inside a living cell in three dimensions.
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Citations
Cites
Precise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Cited by
Precisely and accurately localizing single emitters in fluorescence microscopy
Deschout, H., Zanacchi, F. C., Mlodzianoski, M., Diaspro, A., Bewersdorf, J., Hess, S. T., & Braeckmans, K. (2014) · Nature Methods · doi:10.1038/nmeth.2843 · Cited by 498
Probing cellular events, one quantum dot at a time
Pinaud, F., Clarke, S., Sittner, A., & Dahan, M. (2010) · Nature Methods · doi:10.1038/nmeth.1444 · Cited by 401
Single Cell Optical Imaging and Spectroscopy
Stender, A. S., Marchuk, K., Liu, C., Sander, S., Meyer, M. W., Smith, E. A., Neupane, B., Wang, G., Li, J., Cheng, J.-X., et al. (2013) · Chemical Reviews · doi:10.1021/cr300336e · Cited by 292
Metadata
- Authors
- Thompson, M. A., Lew, M. D., Badieirostami, M., & Moerner, W. E.
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-12-15
- Volume
- 10
- Issue
- 1
- Pages
- 211-218
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- OpenAlex
- W2030396921
- Cited by
- 184
- References
- 1
- FWCI
- 8.25
- Type
- journal article
- Language
- en
- Open access
- green
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- Thompson, M. A., Lew, M. D., Badieirostami, M., & Moerner, W. E. (2010). Localizing and Tracking Single Nanoscale Emitters in Three Dimensions with High Spatiotemporal Resolution Using a Double-Helix Point Spread Function. Nano Letters. https://doi.org/10.1021/nl903295p
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