The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Use of a High-Throughput Screening Approach Coupled with In Vivo Zebrafish Embryo Screening To Develop Hazard Ranking for Engineered Nanomaterials
Open accessAbstract
Because of concerns about the safety of a growing number of engineered nanomaterials (ENM), it is necessary to develop high-throughput screening and in silico data transformation tools that can speed up in vitro hazard ranking. Here, we report the use of a multiparametric, automated screening assay that incorporates sublethal and lethal cellular injury responses to perform high-throughput analysis of a batch of commercial metal/metal oxide nanoparticles (NP) with the inclusion of a quantum dot (QD1). The responses chosen for tracking cellular injury through automated epifluorescence microscopy included ROS production, intracellular calcium flux, mitochondrial depolarization, and plasma membrane permeability. The z-score transformed high volume data set was used to construct heat maps for in vitro hazard ranking as well as showing the similarity patterns of NPs and response parameters through the use of self-organizing maps (SOM). Among the materials analyzed, QD1 and nano-ZnO showed the most prominent lethality, while Pt, Ag, SiO2, Al2O3, and Au triggered sublethal effects but without cytotoxicity. In order to compare the in vitro with the in vivo response outcomes in zebrafish embryos, NPs were used to assess their impact on mortality rate, hatching rate, cardiac rate, and morphological defects. While QDs, ZnO, and Ag induced morphological abnormalities or interfered in embryo hatching, Pt and Ag exerted inhibitory effects on cardiac rate. Ag toxicity in zebrafish differed from the in vitro results, which is congruent with this material's designation as extremely dangerous in the environment. Interestingly, while toxicity in the initially selected QD formulation was due to a solvent (toluene), supplementary testing of additional QDs selections yielded in vitro hazard profiling that reflect the release of chalcogenides. In conclusion, the use of a high-throughput screening, in silico data handling and zebrafish testing may constitute a paradigm for rapid and integrated ENM toxicological screening.
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Cites
Toxic potential of materials at the nanolevel
Nel, A., Xia, T., Mädler, L., & Li, N. (2006) · Science · doi:10.1126/science.1114397 · Cited by 9,324
Self-citationKohonen, T. (1990) · Proceedings of the IEEE · doi:10.1109/5.58325 · Cited by 8,414
Understanding biophysicochemical interactions at the nano–bio interface
Nel, A. E., Mädler, L., Velegol, D., Xia, T., Hoek, E. M. V., Somasundaran, P., Klaessig, F., Castranova, V., & Thompson, M. (2009) · Nature Materials · doi:10.1038/nmat2442 · Cited by 7,083
Self-citationProbing the Cytotoxicity of Semiconductor Quantum Dots
Derfus, A. M., Chan, W. C. W., & Bhatia, S. N. (2004) · Nano Letters · doi:10.1021/nl0347334 · Cited by 3,391
Xia, T., Kovochich, M., Liong, M., Mädler, L., Gilbert, B., Shi, H., Yeh, J. I., Zink, J. I., & Nel, A. E. (2008) · ACS Nano · doi:10.1021/nn800511k · Cited by 2,469
Self-citationA Toxicologic Review of Quantum Dots: Toxicity Depends on Physicochemical and Environmental Factors
Hardman, R. (2006) · Environmental Health Perspectives · doi:10.1289/ehp.8284 · Cited by 2,303
In vitro toxicity of nanoparticles in BRL 3A rat liver cells
Hussain, S. M., Hess, K. L., Gearhart, J. M., Geiss, K. T., & Schlager, J. J. (2005) · Toxicology in Vitro · doi:10.1016/j.tiv.2005.06.034 · Cited by 2,025
Xia, T., Kovochich, M., Brant, J., Hotze, M., Sempf, J., Oberley, T., Sioutas, C., Yeh, J. I., Wiesner, M. R., & Nel, A. E. (2006) · Nano Letters · doi:10.1021/nl061025k · Cited by 1,918
Self-citationCytotoxicity of Colloidal CdSe and CdSe/ZnS Nanoparticles
Kirchner, C., Liedl, T., Kudera, S., Pellegrino, T., Muñoz Javier, A., Gaub, H. E., Stölzle, S., Fertig, N., & Parak, W. J. (2005) · Nano Letters · doi:10.1021/nl047996m · Cited by 1,619
Toxicity testing in the 21st century: A vision and a strategy
Gibb, S. (2008) · Reproductive Toxicology · doi:10.1016/j.reprotox.2007.10.013 · Cited by 1,257
Cited by
Sharifi, S., Behzadi, S., Laurent, S., Laird Forrest, M., Stroeve, P., & Mahmoudi, M. (2012) · Chem. Soc. Rev. · doi:10.1039/c1cs15188f · Cited by 1,460
Enzyme-responsive nanoparticles for drug release and diagnostics
de la Rica, R., Aili, D., & Stevens, M. M. (2012) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.01.002 · Cited by 765
The Use of Alternative Strategies for Enhanced Nanoparticle Delivery to Solid Tumors
Izci, M., Maksoudian, C., Manshian, B. B., & Soenen, S. J. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00779 · Cited by 506
Toxicity of Nanomaterials: Exposure, Pathways, Assessment, and Recent Advances
Ganguly, P., Breen, A., & Pillai, S. C. (2018) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.8b00068 · Cited by 341
Demokritou, P., Gass, S., Pyrgiotakis, G., Cohen, J. M., Goldsmith, W., McKinney, W., Frazer, D., Ma, J., Schwegler-Berry, D., Brain, J., et al. (2013) · Nanotoxicology · doi:10.3109/17435390.2012.739665 · Cited by 152
Metadata
- Authors
- George, S., Xia, T., Rallo, R., Zhao, Y., Ji, Z., Lin, S., Wang, X., Zhang, H., France, B., Schoenfeld, D., Damoiseaux, R., Liu, R., Lin, S., Bradley, K. A., Cohen, Y., & Nel, A. E.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-02-16
- Volume
- 5
- Issue
- 3
- Pages
- 1805-1817
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W2036661308
- Cited by
- 326
- References
- 45
- FWCI
- 18.13
- Type
- journal article
- Language
- en
- Open access
- green
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- George, S., Xia, T., Rallo, R., Zhao, Y., Ji, Z., Lin, S., Wang, X., Zhang, H., France, B., Schoenfeld, D., Damoiseaux, R., Liu, R., Lin, S., Bradley, K. A., Cohen, Y., & Nel, A. E. (2011). Use of a High-Throughput Screening Approach Coupled with In Vivo Zebrafish Embryo Screening To Develop Hazard Ranking for Engineered Nanomaterials. ACS Nano. https://doi.org/10.1021/nn102734s
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