The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Cytotoxic Origin of Copper(II) Oxide Nanoparticles: Comparative Studies with Micron-Sized Particles, Leachate, and Metal Salts
Abstract
The work investigates the source of toxicity of copper oxide nanoparticles (CuO NPs) with respect to its leaching characteristic and speciation. Complexation-mediated leaching of CuO NPs by amino acids was identified as the source of toxicity toward Escherichia coli, the model microorganism used in the current study. The leached copper-peptide complex induces a multiple-fold increase in intracellular reactive oxygen species generation and reduces the fractions of viable cells, resulting in the overall inhibition of biomass growth. The cytotoxicity of the complex leachate is however different from that of equivalent soluble copper salts (nitrates and sulfates). A pH-dependent copper speciation during the addition of copper salts gives rise to uncoordinated copper ions, which in turn result in greater toxicity and cell lysis, the latter of which was not observed for CuO NPs even at comparable pH. Since leaching did not occur with micrometer-sized CuO, no cytotoxicty effect was observed, thus highlighting the prominence of materials toxicity at the nanoscale.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Toxic potential of materials at the nanolevel
Nel, A., Xia, T., Mädler, L., & Li, N. (2006) · Science · doi:10.1126/science.1114397 · Cited by 9,324
Xia, T., Kovochich, M., Liong, M., Mädler, L., Gilbert, B., Shi, H., Yeh, J. I., Zink, J. I., & Nel, A. E. (2008) · ACS Nano · doi:10.1021/nn800511k · Cited by 2,469
The potential environmental impact of engineered nanomaterials
Colvin, V. L. (2003) · Nature Biotechnology · doi:10.1038/nbt875 · Cited by 2,158
DNA damage and oxygen radical toxicity
Imlay, J. A., & Linn, S. (1988) · Science · doi:10.1126/science.3287616 · Cited by 1,974
FOYER, C. H., & NOCTOR, G. (2005) · Plant, Cell & Environment · doi:10.1111/j.1365-3040.2005.01327.x · Cited by 1,887
Characterisation of copper oxide nanoparticles for antimicrobial applications
Ren, G., Hu, D., Cheng, E. W. C., Vargas-Reus, M. A., Reip, P., & Allaker, R. P. (2009) · International Journal of Antimicrobial Agents · doi:10.1016/j.ijantimicag.2008.12.004 · Cited by 1,604
Heinlaan, M., Ivask, A., Blinova, I., Dubourguier, H.-C., & Kahru, A. (2008) · Chemosphere · doi:10.1016/j.chemosphere.2007.11.047 · Cited by 1,522
Toxicity of Silver Nanoparticles to Chlamydomonas reinhardtii
Navarro, E., Piccapietra, F., Wagner, B., Marconi, F., Kaegi, R., Odzak, N., Sigg, L., & Behra, R. (2008) · Environmental Science & Technology · doi:10.1021/es801785m · Cited by 1,457
Toxicity of nanoparticles of CuO, ZnO and TiO2 to microalgae Pseudokirchneriella subcapitata
Aruoja, V., Dubourguier, H.-C., Kasemets, K., & Kahru, A. (2009) · Science of The Total Environment · doi:10.1016/j.scitotenv.2008.10.053 · Cited by 1,259
Proton-dependent multidrug efflux systems
Paulsen, I. T., Brown, M. H., & Skurray, R. A. (1996) · Microbiological Reviews · doi:10.1128/mmbr.60.4.575-608.1996 · Cited by 1,198
Transition Metal Speciation in the Cell: Insights from the Chemistry of Metal Ion Receptors
Finney, L. A., & O'Halloran, T. V. (2003) · Science · doi:10.1126/science.1085049 · Cited by 1,100
Oxidative stress responses in Escherichia coli and Salmonella typhimurium
Farr, S. B., & Kogoma, T. (1991) · Microbiological Reviews · doi:10.1128/mmbr.55.4.561-585.1991 · Cited by 1,091
Handbook of fluorescent probes and research products
Haugland, R. P. (2002) · Cited by 1,036
Limbach, L. K., Wick, P., Manser, P., Grass, R. N., Bruinink, A., & Stark, W. J. (2007) · Environmental Science & Technology · doi:10.1021/es062629t · Cited by 889
Antibacterial Activity of Nanosilver Ions and Particles
Sotiriou, G. A., & Pratsinis, S. E. (2010) · Environmental Science & Technology · doi:10.1021/es101072s · Cited by 832
Haynes, L. J. (1983) · Endeavour · doi:10.1016/0160-9327(83)90064-9 · Cited by 799
Hodgson, E. K., & Fridovich, I. (1975) · Biochemistry · doi:10.1021/bi00695a010 · Cited by 782
Flame spray pyrolysis: An enabling technology for nanoparticles design and fabrication
Teoh, W. Y., Amal, R., & Mädler, L. (2010) · Nanoscale · doi:10.1039/c0nr00017e · Cited by 731
Self-citationMetal-induced oxidative stress and signal transduction
Leonard, S. S., Harris, G. K., & Shi, X. (2004) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2004.09.010 · Cited by 663
Toxicity of nanoparticles of ZnO, CuO and TiO2 to yeast Saccharomyces cerevisiae
Kasemets, K., Ivask, A., Dubourguier, H.-C., & Kahru, A. (2009) · Toxicology in Vitro · doi:10.1016/j.tiv.2009.05.015 · Cited by 627
Myhre, O., Andersen, J. M., Aarnes, H., & Fonnum, F. (2003) · Biochemical Pharmacology · doi:10.1016/s0006-2952(03)00083-2 · Cited by 623
Generation of Superoxide Free Radical during the Autoxidation of Thiols
Misra, H. P. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42810-x · Cited by 556
Outten, F. W., Huffman, D. L., Hale, J. A., & O'Halloran, T. V. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m104122200 · Cited by 544
Molecular Analysis of the Copper-Transporting Efflux System CusCFBA of Escherichia coli
Franke, S., Grass, G., Rensing, C., & Nies, D. H. (2003) · Journal of Bacteriology · doi:10.1128/jb.185.13.3804-3812.2003 · Cited by 538
CopA: An Escherichia coli Cu(I)-translocating P-type ATPase
Rensing, C., Fan, B., Sharma, R., Mitra, B., & Rosen, B. P. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.2.652 · Cited by 516
Bacterial Programmed Cell Death and Multicellular Behavior in Bacteria
Engelberg-Kulka, H., Amitai, S., Kolodkin-Gal, I., & Hazan, R. (2006) · PLoS Genetics · doi:10.1371/journal.pgen.0020135 · Cited by 425
A Novel Anti-Influenza Copper Oxide Containing Respiratory Face Mask
Borkow, G., Zhou, S. S., Page, T., & Gabbay, J. (2010) · PLoS ONE · doi:10.1371/journal.pone.0011295 · Cited by 374
Intracellular Copper Does Not Catalyze the Formation of Oxidative DNA Damage in Escherichia coli
Macomber, L., Rensing, C., & Imlay, J. A. (2007) · Journal of Bacteriology · doi:10.1128/jb.01357-06 · Cited by 356
CuO–cotton nanocomposite: Formation, morphology, and antibacterial activity
Perelshtein, I., Applerot, G., Perkas, N., Wehrschuetz-Sigl, E., Hasmann, A., Guebitz, G., & Gedanken, A. (2009) · Surface and Coatings Technology · doi:10.1016/j.surfcoat.2009.06.028 · Cited by 329
(1990) · Royal Society of Chemistry eBooks · doi:10.1039/9781847557483 · Cited by 298
Contribution of Copper Ion Resistance to Survival of Escherichia coli on Metallic Copper Surfaces
Santo, C. E., Taudte, N., Nies, D. H., & Grass, G. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.01938-07 · Cited by 297
Electronic structure of plastocyanin: excited state spectral features
Gewirth, A. A., & Solomon, E. I. (1988) · Journal of the American Chemical Society · doi:10.1021/ja00220a015 · Cited by 260
Copper Oxide Impregnated Textiles with Potent Biocidal Activities
Gabbay, J., Borkow, G., Mishal, J., Magen, E., Zatcoff, R., & Shemer-Avni, Y. (2006) · Journal of Industrial Textiles · doi:10.1177/1528083706060785 · Cited by 255
Escherichia coli mazEF -Mediated Cell Death Is Triggered by Various Stressful Conditions
Hazan, R., Sat, B., & Engelberg-Kulka, H. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.11.3663-3669.2004 · Cited by 254
Li, M., Pokhrel, S., Jin, X., Mädler, L., Damoiseaux, R., & Hoek, E. M. V. (2011) · Environmental Science & Technology · doi:10.1021/es102266g · Cited by 231
Cuppett, J. D. (2006) · Chemical Senses · doi:10.1093/chemse/bjl010 · Cited by 227
Escherichia coli expresses a copper- and zinc-containing superoxide dismutase.
Benov, L. T., & Fridovich, I. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47248-1 · Cited by 203
Synthesis, Characterization, and Antifouling Potential of Functionalized Copper Nanoparticles
Anyaogu, K. C., Fedorov, A. V., & Neckers, D. C. (2008) · Langmuir · doi:10.1021/la800102f · Cited by 201
The regulation and role of the periplasmic copper, zinc superoxide dismutase of Escherichia coli
Gort, A. S., Ferber, D. M., & Imlay, J. A. (1999) · Molecular Microbiology · doi:10.1046/j.1365-2958.1999.01343.x · Cited by 191
Hydrogen peroxide as a potent bacteriostatic antibiotic: Implications for host defense
Hyslop, P. A., Hinshaw, D. B., Scraufstatter, I. U., Cochrane, C. G., Kunz, S., & Vosbeck, K. (1995) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(95)00005-i · Cited by 182
Teoh, W. Y., Mädler, L., Beydoun, D., Pratsinis, S. E., & Amal, R. (2005) · Chemical Engineering Science · doi:10.1016/j.ces.2005.05.037 · Cited by 180
Self-citationTHE ROLE OF REACTIVE OXYGEN SPECIES IN COPPER TOXICITY TO TWO FRESHWATER GREEN ALGAE1
Knauert, S., & Knauer, K. (2008) · Journal of Phycology · doi:10.1111/j.1529-8817.2008.00471.x · Cited by 175
Reversible Antimicrobial Photoswitching in Nanosilver
Gunawan, C., Teoh, W. Y., Marquis, C. P., Lifia, J., & Amal, R. (2009) · Small · doi:10.1002/smll.200801202 · Cited by 171
Self-citationLaCroix, L. B., Randall, D. W., Nersissian, A. M., Hoitink, C. W. G., Canters, G. W., Valentine, J. S., & Solomon, E. I. (1998) · Journal of the American Chemical Society · doi:10.1021/ja980606b · Cited by 140
Inactivation of E. coli mediated by high surface area CuO accelerated by light irradiation >360 nm
Paschoalino, M. P., Guedes, N. C., Jardim, W. F., Mielczarski, E., Mielczarski, J. A., Bowen, P., & Kiwi, J. T. (2008) · Journal of Photochemistry and Photobiology A Chemistry · doi:10.1016/j.jphotochem.2008.05.010 · Cited by 93
Generation of hydrogen peroxide by incidental metal ion-catalyzed autooxidation of glutathione
Albro, P. W., Corbett, J. T., & Schroeder, J. L. (1986) · Journal of Inorganic Biochemistry · doi:10.1016/0162-0134(86)80060-5 · Cited by 57
Repair response of Escherichia coli to hydrogen peroxide DNA damage
Hagensee, M. E., & Moses, R. E. (1986) · Journal of Bacteriology · doi:10.1128/jb.168.3.1059-1065.1986 · Cited by 32
Cinnamaldehyde Induces PCD-Like Death of Microcystis aeruginosa via Reactive Oxygen Species
Hu, L., Zhou, W., Yang, J. D., Chen, J., Yin, Y. F., & Shi, Z. Q. (2010) · Water Air & Soil Pollution · doi:10.1007/s11270-010-0571-1 · Cited by 32
Kydd, R., Teoh, W. Y., Scott, J. A., Ferri, D., & Amal, R. (2009) · ChemCatChem · doi:10.1002/cctc.200900099 · Cited by 24
Self-citation
Cited by
Antimicrobial activity of metals: mechanisms, molecular targets and applications
Lemire, J. A., Harrison, J. J., & Turner, R. J. (2013) · Nature Reviews Microbiology · doi:10.1038/nrmicro3028 · Cited by 2,746
Toxic mechanisms of copper oxide nanoparticles in epithelial kidney cells
Thit, A., Selck, H., & Bjerregaard, H. F. (2015) · Toxicology in Vitro · doi:10.1016/j.tiv.2015.03.020 · Cited by 85
Yang, J., Huang, Z., Tan, J., Pan, J., Chen, S., & Wan, W. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2023.10.005 · Cited by 83
Levak, M., Burić, P., Dutour Sikirić, M., Domazet Jurašin, D., Mikac, N., Bačić, N., Drexel, R., Meier, F., Jakšić, Ž., & Lyons, D. M. (2017) · Environmental Science & Technology · doi:10.1021/acs.est.6b03161 · Cited by 69
Wonoputri, V., Gunawan, C., Liu, S., Barraud, N., Yee, L. H., Lim, M., & Amal, R. (2015) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b07971 · Cited by 38
Self-citation
Metadata
- Authors
- Gunawan, C., Teoh, W. Y., Marquis, C. P., & Amal, R.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-08-12
- Volume
- 5
- Issue
- 9
- Pages
- 7214-7225
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W1987154750
- Cited by
- 349
- References
- 49
- FWCI
- 7.13
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
