The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Quantum Dot–Fluorescent Protein FRET Probes for Sensing Intracellular pH
Open accessAbstract
Intracellular pH (pH(i)) plays a critical role in the physiological and pathophysiological processes of cells, and fluorescence imaging using pH-sensitive indicators provides a powerful tool to assess the pH(i) of intact cells and subcellular compartments. Here we describe a nanoparticle-based ratiometric pH sensor, comprising a bright and photostable semiconductor quantum dot (QD) and pH-sensitive fluorescent proteins (FPs), exhibiting dramatically improved sensitivity and photostability compared to BCECF, the most widely used fluorescent dye for pH imaging. We found that Förster resonance energy transfer between the QD and multiple FPs modulates the FP/QD emission ratio, exhibiting a >12-fold change between pH 6 and 8. The modularity of the probe enables customization to specific biological applications through genetic engineering of the FPs, as illustrated by the altered pH range of the probe through mutagenesis of the fluorescent protein. The QD-FP probes facilitate visualization of the acidification of endosomes in living cells following polyarginine-mediated uptake. These probes have the potential to enjoy a wide range of intracellular pH imaging applications that may not be feasible with fluorescent proteins or organic fluorophores alone.
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Cites
Principles of Fluorescence Spectroscopy
(2006) · Springer eBooks · doi:10.1007/978-0-387-46312-4 · Cited by 19,339
Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N. G., Palmer, A. E., & Tsien, R. Y. (2004) · Nature Biotechnology · doi:10.1038/nbt1037 · Cited by 4,619
Fluorescent Indicators for Intracellular pH
Han, J., & Burgess, K. (2010) · Chemical Reviews · doi:10.1021/cr900249z · Cited by 1,620
(2008) · Elsevier eBooks · doi:10.1016/b978-0-12-370501-3.x0001-x · Cited by 1,367
Panyam, J., Zhou, W., Prabha, S., Sahoo, S. K., & Labhasetwar, V. (2002) · The FASEB Journal · doi:10.1096/fj.02-0088com · Cited by 1,044
Improving the photostability of bright monomeric orange and red fluorescent proteins
Shaner, N. C., Lin, M. Z., McKeown, M. R., Steinbach, P. A., Hazelwood, K. L., Davidson, M. W., & Tsien, R. Y. (2008) · Nature Methods · doi:10.1038/nmeth.1209 · Cited by 1,009
Cellular pH regulators: potentially promising molecular targets for cancer chemotherapy
Izumi, H., Torigoe, T., Ishiguchi, H., Uramoto, H., Yoshida, Y., Tanabe, M., Ise, T., Murakami, T., Yoshida, T., Nomoto, M., et al. (2003) · Cancer Treatment Reviews · doi:10.1016/s0305-7372(03)00106-3 · Cited by 613
Quantum dot-based resonance energy transfer and its growing application in biology
Medintz, I. L., & Mattoussi, H. (2009) · Phys. Chem. Chem. Phys. · doi:10.1039/b813919a · Cited by 569
A Ratiometric CdSe/ZnS Nanocrystal pH Sensor
Snee, P. T., Somers, R. C., Nair, G., Zimmer, J. P., Bawendi, M. G., & Nocera, D. G. (2006) · Journal of the American Chemical Society · doi:10.1021/ja0618999 · Cited by 510
Medintz, I. L., Stewart, M. H., Trammell, S. A., Susumu, K., Delehanty, J. B., Mei, B. C., Melinger, J. S., Blanco-Canosa, J. B., Dawson, P. E., & Mattoussi, H. (2010) · Nature Materials · doi:10.1038/nmat2811 · Cited by 473
Cited by
Ratiometric optical nanoprobes enable accurate molecular detection and imaging
Huang, X., Song, J., Yung, B. C., Huang, X., Xiong, Y., & Chen, X. (2018) · Chemical Society Reviews · doi:10.1039/c7cs00612h · Cited by 820
Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications
Steinegger, A., Wolfbeis, O. S., & Borisov, S. M. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00451 · Cited by 622
Förster Resonance Energy Transfer between Quantum Dot Donors and Quantum Dot Acceptors
Chou, K., & Dennis, A. (2015) · Sensors · doi:10.3390/s150613288 · Cited by 302
Self-citationMaxwell, T., Nogueira Campos, M. G., Smith, S., Doomra, M., Thwin, Z., & Santra, S. (2020) · Nanoparticles for Biomedical Applications · doi:10.1016/b978-0-12-816662-8.00015-1 · Cited by 43
Metadata
- Authors
- Dennis, A. M., Rhee, W. J., Sotto, D., Dublin, S. N., & Bao, G.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-04-02
- Volume
- 6
- Issue
- 4
- Pages
- 2917-2924
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W2046584498
- Cited by
- 340
- References
- 30
- FWCI
- 16.65
- Type
- journal article
- Language
- en
- Open access
- green
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