Halothane binding proteome in human brain cortex
Abstract
Inhaled anesthetics bind specifically to a wide variety of proteins in the brain. This set of proteins must include those that contribute to the physiological and behavioral phenotypes of anesthesia and the related side effects. To identify the anesthetic-binding targets and functional pathways associated with these targets in human brain, halothane photolabeling and two-dimensional (2D) gel electrophoresis were used. Both membrane and soluble proteins from human temporal cortex were prepared. More than 300 membrane and 400 soluble protein spots were detected on the stained blots, of which 23 membrane and 34 soluble proteins were labeled by halothane and identified by mass spectroscopy. Their functional classification reveals five groups, including carbohydrate metabolism, protein folding, oxidative phosphorylation, nucleoside triphosphatase, and dimer/kinase activity with different correlative stringency. When network analysis of the interaction between these protein molecules is used, the weighted interaction accentuates the cellular protein components important in cell growth and proliferation, cell cycle and cell death, and cell-cell signaling and interactions, although no pathway was specific. This study provides evidence for multiple anesthetic binding targets and suggests potential pathways involved in their actions.
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Actual evidence (e.g. from genomics and proteomics) points to anesthetic action in microtubules.
“Historically, views of anesthetic action have focused on neuronal membrane proteins, but actual evidence (e.g. from genomics and proteomics) [84,85] points to anesthetic action in microtubules.”
From Consciousness in the universe (2014) · cites this paper as [85]
Anesthetics bind to tubulin and destabilize microtubules.
“Anesthetics bind to tubulin, causing microtubules to destabilize [84, 85].”
From The Biology of General Anesthesia from Paramecium to Primate (2019) · cites this paper as [84]
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- Authors
- Pan, J. Z., Xi, J., Tobias, J. W., Eckenhoff, M. F., & Eckenhoff, R. G.
- Affiliations
- University of Pennsylvania Health System (US)
- Department of Anesthesiology and Critical Care and Department of Bioinformatics, University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104
- Corresponding author
- Pan, Jonathan Z.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-12-19
- Volume
- 6
- Issue
- 2
- Pages
- 582-592
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W1975765816
- Cited by
- 49
- References
- 49
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 1 · 2023: 2 · 2022: 6 · 2021: 2 · 2019: 4 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 2
- FWCI
- 0.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Proteome Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM 51595, R01GM051595)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Anesthesia and Neurotoxicity Research
- Health Sciences › Medicine › Anesthesiology and Pain Medicine › Anesthesia and Sedative Agents
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- halothane, cerebral cortex, temporal cortex, protein-protein interaction network, membrane proteins, soluble protein, carbohydrate metabolism, protein folding, oxidative phosphorylation, nucleoside triphosphatase, kinase activity, cell cycle, cell death, signal transduction, anesthetic side effects
- MeSH terms
- Cerebral Cortex, Enzymes, Halothane, Humans, Nerve Tissue Proteins, Protein Binding, Electrophoresis, Gel, Two-Dimensional, Proteome
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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