Inhalational anesthetic-binding proteins in rat neuronal membranes
Open accessAbstract
Molecular targets of inhaled anesthetics must be represented in the group that specifically bind these drugs, but the paucity of direct binding data has limited the number of candidates for further evaluation. To find candidate targets, we used a combination of photolabeling, two-dimensional gel electrophoresis, and mass spectrometry to identify halothane-binding targets in rat neuronal membranes. Of the 265 spots detected on the two-dimensional gels, 90 were labeled by [(14)C]halothane, and 34 were identified. Mitochondrial proteins, especially respiratory complex and voltage-dependent anion channels, dominated the labeled group, and there were several examples of subunit- and state-dependent binding. A significant correlation was found between internal protein cavities and binding in a group of proteins with high resolution structures. Therefore, in addition to identifying novel neuronal targets, these data suggest a general molecular feature, the buried cavity, as a dominant attribute of volatile anesthetic-binding sites found in a limited number of neuronal membrane proteins.
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Actual evidence (e.g. from genomics and proteomics) points to anesthetic action in microtubules.
“Historically, views of anesthetic action have focused on neuronal membrane proteins, but actual evidence (e.g. from genomics and proteomics) [84,85] points to anesthetic action in microtubules.”
From Consciousness in the universe (2014) · cites this paper as [84]
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- Authors
- Xi, J., Liu, R., Asbury, G. R., Eckenhoff, M. F., & Eckenhoff, R. G.
- Affiliations
- University of Pennsylvania Health System (US)
- Corresponding author
- Eckenhoff, Roderic G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2004-05
- Volume
- 279
- Issue
- 19
- Pages
- 19628-19633
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2045309711
- Cited by
- 54
- References
- 42
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 5 · 2021: 1 · 2019: 4 · 2018: 1 · 2017: 2 · 2015: 2 · 2014: 3 · 2013: 5 · 2012: 6
- FWCI
- 5.17
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM 55876, GM 51595, R01GM051595, P01GM055876)
- Topics
- Health Sciences › Medicine › Anesthesiology and Pain Medicine › Anesthesia and Sedative Agents
- Life Sciences › Neuroscience › Developmental Neuroscience › Anesthesia and Neurotoxicity Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- inhalational anesthetics, halothane, anesthetic binding sites, neuronal membrane, mitochondrial proteins, respiratory chain complexes, voltage-dependent anion channel, protein cavities, photolabeling, binding site identification
- MeSH terms
- Anesthetics, Animals, Brain, Carrier Proteins, Cell Membrane, Halothane, Male, Mitochondria, Neurons, Protein Binding, Mass Spectrometry, Electrophoresis, Gel, Two-Dimensional, Rats, Wistar, Protein Structure, Tertiary, Anesthetics, Inhalation, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Rats
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Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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