The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Quantitative Analysis of the Intra- and Inter-Individual Variability of the Normal Urinary Proteome
Open accessAbstract
Urine is a readily and noninvasively obtainable body fluid. Mass spectrometry (MS)-based proteomics has shown that urine contains thousands of proteins. Urine is a potential source of biomarkers for diseases of proximal and distal tissues but it is thought to be more variable than the more commonly used plasma. By LC-MS/MS analysis on an LTQ-Orbitrap without prefractionation we characterized the urinary proteome of seven normal human donors over three consecutive days. Label-free quantification of triplicate single runs covered the urinary proteome to a depth of more than 600 proteins. The median coefficient of variation (cv) of technical replicates was 0.18. Interday variability was markedly higher with a cv of 0.48 and the overall variation of the urinary proteome between individuals was 0.66. Thus technical variability in our data was 7.5%, whereas intrapersonal variability contributed 45.5% and interpersonal variability contributed 47.1% to total variability. Determination of the normal fluctuation of individual urinary proteins should be useful in establishing significance thresholds in biomarker studies. Our data also allowed definition of a common and abundant set of 500 proteins that were readily detectable in all studied individuals. This core urinary proteome has a high proportion of secreted, membrane, and relatively high-molecular weight proteins.
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Cox, J., & Mann, M. (2008) · Nature Biotechnology · doi:10.1038/nbt.1511 · Cited by 14,855
Self-citationUniversal sample preparation method for proteome analysis
Wiśniewski, J. R., Zougman, A., Nagaraj, N., & Mann, M. (2009) · Nature Methods · doi:10.1038/nmeth.1322 · Cited by 8,352
Self-citationMass spectrometry-based proteomics
Aebersold, R., & Mann, M. (2003) · Nature · doi:10.1038/nature01511 · Cited by 6,955
Self-citationMicroarray data analysis: from disarray to consolidation and consensus
Allison, D. B., Cui, X., Page, G. P., & Sabripour, M. (2006) · Nature Reviews Genetics · doi:10.1038/nrg1749 · Cited by 1,339
Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast
de Godoy, L. M. F., Olsen, J. V., Cox, J., Nielsen, M. L., Hubner, N. C., Fröhlich, F., Walther, T. C., & Mann, M. (2008) · Nature · doi:10.1038/nature07341 · Cited by 873
Self-citationQuantitative proteomics reveals subset-specific viral recognition in dendritic cells
Luber, C. A., Cox, J., Lauterbach, H., Fancke, B., Selbach, M., Tschopp, J., Akira, S., Wiegand, M., Hochrein, H., O'Keeffe, M., et al. (2010) · Immunity · doi:10.1016/j.immuni.2010.01.013 · Cited by 576
Self-citationProteome-wide cellular protein concentrations of the human pathogen Leptospira interrogans
Malmström, J., Beck, M., Schmidt, A., Lange, V., Deutsch, E. W., & Aebersold, R. (2009) · Nature · doi:10.1038/nature08184 · Cited by 421
Practical Points in Urinary Proteomics
Thongboonkerd, V. (2007) · Journal of Proteome Research · doi:10.1021/pr070328s · Cited by 208
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Erdbrügger, U., Blijdorp, C. J., Bijnsdorp, I. V., Borràs, F. E., Burger, D., Bussolati, B., Byrd, J. B., Clayton, A., Dear, J. W., Falcón‐Pérez, J. M., et al. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12093 · Cited by 404
High-sensitivity Analytical Approaches for the Structural Characterization of Glycoproteins
Alley, W. R., Mann, B. F., & Novotny, M. V. (2013) · Chemical Reviews · doi:10.1021/cr3003714 · Cited by 310
Vidal, M., Chan, D. W., Gerstein, M., Mann, M., Omenn, G. S., Tagle, D., Sechi, S., & Participants, W. (2012) · Clinical Proteomics · doi:10.1186/1559-0275-9-6 · Cited by 41
Self-citationProstate cancer reshapes the secreted and extracellular vesicle urinary proteomes
Khoo, A., Govindarajan, M., Qiu, Z., Liu, L. Y., Ignatchenko, V., Waas, M., Macklin, A., Keszei, A., Neu, S., Main, B. P., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-49424-5 · Cited by 40
Metadata
- Authors
- Nagaraj, N., & Mann, M.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-01-05
- Volume
- 10
- Issue
- 2
- Pages
- 637-645
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2062311239
- Cited by
- 252
- References
- 8
- FWCI
- 6.77
- Type
- journal article
- Language
- en
- Open access
- green
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