The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Comparative and Targeted Proteomic Analyses of Urinary Microparticles from Bladder Cancer and Hernia Patients
Abstract
Bladder cancer is a common urologic cancer whose incidence continues to rise annually. Urinary microparticles are an attractive material for noninvasive bladder cancer biomarker discovery. In this study, we applied isotopic dimethylation labeling coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to discover bladder cancer biomarkers in urinary microparticles isolated from hernia (control) and bladder cancer patients. This approach identified 2964 proteins based on more than two distinct peptides, of which 2058 had not previously been reported as constituents of human urine exosomes/microparticles. A total of 107 differentially expressed proteins were identified as candidate biomarkers. Differences in the concentrations of 29 proteins (41 signature peptides) were precisely quantified by LC-MRM/MS in 48 urine samples of bladder cancer, hernia, and urinary tract infection/hematuria. Concentrations of 24 proteins changed significantly (p<0.05) between bladder cancer (n=28) and hernia (n=12), with area-under-the-curve values ranging from 0.702 to 0.896. Finally, we quantified tumor-associated calcium-signal transducer 2 (TACSTD2) in raw urine specimens (n=221) using a commercial ELISA and confirmed its potential value for diagnosis of bladder cancer. Our study reveals a strong association of TACSTD2 with bladder cancer and highlights the potential of human urinary microparticles in the noninvasive diagnosis of bladder cancer.
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Cox, J., & Mann, M. (2008) · Nature Biotechnology · doi:10.1038/nbt.1511 · Cited by 14,855
Jemal, A., Siegel, R., Xu, J., & Ward, E. (2010) · CA: A Cancer Journal for Clinicians · doi:10.3322/caac.20073 · Cited by 12,596
Jemal, A., Siegel, R., Ward, E., Hao, Y., Xu, J., & Thun, M. J. (2009) · CA: A Cancer Journal for Clinicians · doi:10.3322/caac.20006 · Cited by 9,709
Cited by
Evidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine
Fais, S., O’Driscoll, L., Borras, F. E., Buzas, E., Camussi, G., Cappello, F., Carvalho, J., Cordeiro da Silva, A., Del Portillo, H., El Andaloussi, S., et al. (2016) · ACS Nano · doi:10.1021/acsnano.5b08015 · Cited by 481
Erdbrügger, U., Blijdorp, C. J., Bijnsdorp, I. V., Borràs, F. E., Burger, D., Bussolati, B., Byrd, J. B., Clayton, A., Dear, J. W., Falcón‐Pérez, J. M., et al. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12093 · Cited by 404
Corrado, C., Raimondo, S., Chiesi, A., Ciccia, F., De Leo, G., & Alessandro, R. (2013) · International Journal of Molecular Sciences · doi:10.3390/ijms14035338 · Cited by 396
Applications of targeted proteomics in systems biology and translational medicine
Ebhardt, H. A., Root, A., Sander, C., & Aebersold, R. (2015) · PROTEOMICS · doi:10.1002/pmic.201500004 · Cited by 184
Developing proteomic biomarkers for bladder cancer: towards clinical application
Frantzi, M., Latosinska, A., Flühe, L., Hupe, M. C., Critselis, E., Kramer, M. W., Merseburger, A. S., Mischak, H., & Vlahou, A. (2015) · Nature Reviews Urology · doi:10.1038/nrurol.2015.100 · Cited by 83
Metadata
- Authors
- Chen, C.-L., Lai, Y.-F., Tang, P., Chien, K.-Y., Yu, J.-S., Tsai, C.-H., Chen, H.-W., Wu, C.-C., Chung, T., Hsu, C.-W., Chen, C.-D., Chang, Y.-S., Chang, P.-L., & Chen, Y.-T.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-10-31
- Volume
- 11
- Issue
- 12
- Pages
- 5611-5629
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2095387199
- Cited by
- 212
- References
- 3
- FWCI
- 5.58
- Type
- journal article
- Language
- en
- Open access
- closed
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