The Conserved Set of Host Proteins Incorporated into HIV-1 Virions Suggests a Common Egress Pathway in Multiple Cell Types
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HIV-1 incorporates a large array of host proteins into virions. Determining the host protein composition in HIV virions has technical difficulties, including copurification of microvesicles. We developed an alternative purification technique using cholesterol that differentially modulates the density of virions and microvesicles (density modification, DM) allowing for high-yield virion purification that is essential for tandem mass spectrometric and quantitative proteomic (iTRAQ) analysis. DM purified virions were analyzed using iTRAQ and validated against Optiprep (60% iodixanol) purified virions. We were able to characterize host protein incorporation in DM-purified HIV particles derived from CD4+ T-cell lines; we compared this data set to a reprocessed data set of monocyte-derived macrophages (MDM) derived HIV-1 using the same bioinformatics pipeline. Seventy-nine clustered proteins were shared between the MDM derived and T-cell derived data set. These clusters included an extensive collection of actin isoforms, HLA proteins, chaperones, and a handful of other proteins, many of which have previously been documented to interact with viral proteins. Other proteins of note were ERM proteins, the dynamin domain containing protein EH4, a phosphodiesterase, and cyclophilin A. As these proteins are incorporated in virions produced in both cell types, we hypothesize that these proteins may have direct interactions with viral proteins or may be important in the viral life cycle. Additionally, identified common set proteins are predicted to interact with >1000 related human proteins. Many of these secondary interacting proteins are reported to be incorporated into virions, including ERM proteins and adhesion molecules. Thus, only a few direct interactions between host and viral proteins may dictate the host protein composition in virions. Ultimately, interaction and expression differences in host proteins between cell types may drive virion phenotypic diversity, despite conserved viral protein-host protein interactions between cell types.
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These developments helped establish that virions contain host proteins.
“These developments have helped establish that virions contain host proteins and have triggered the expansion of MS studies to the proteomes of filoviruses, HIV, influenza, and respiratory syncytial virus (16–22).”
From The Impact of Mass Spectrometry–Based Proteomics on Fundamental Discoveries in Virology (2014) · cites this paper as [18]
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Cites
The WEKA data mining software: an update
Hall, M., Frank, E., Holmes, G., Pfahringer, B., Reutemann, P., & Witten, I. H. (2009) · ACM SIGKDD Explorations Newsletter · doi:10.1145/1656274.1656278 · Cited by 17,750 · see citing works
Escola, J.-M., Kleijmeer, M. J., Stoorvogel, W., Griffith, J. M., Yoshie, O., & Geuze, H. J. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.32.20121 · Cited by 1,228 · see citing works
The Protein Network of HIV Budding
von Schwedler, U. K., Stuchell‐Brereton, M. D., Müller, B., Ward, D. M., Chung, H.-Y., Morita, E., Wang, H. E., Davis, T., He, G.-P., Cimbora, D. M., et al. (2003) · Cell · doi:10.1016/s0092-8674(03)00714-1 · Cited by 815 · see citing works
Human immunodeficiency virus type 1 Gag protein binds to cyclophilins A and B
Luban, J., Bossolt, K. L., Franke, E. K., Kalpana, G. V., & Goff, S. P. (1993) · Cell · doi:10.1016/0092-8674(93)90637-6 · Cited by 792 · see citing works
The minimum information about a proteomics experiment (MIAPE)
Taylor, C. F., Paton, N. W., Lilley, K. S., Binz, P., Julian, R. K., Jones, A. R., Zhu, W., Apweiler, R., Aebersold, R. H., Deutsch, E. W., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1329 · Cited by 769 · see citing works
Specific incorporation of cyclophilin A into HIV-1 virions
Franke, E. K., Yuan, H. E. H., & Luban, J. (1994) · Nature · doi:10.1038/372359a0 · Cited by 745 · see citing works
The Regulation of Exosome Secretion: a Novel Function of the p53 Protein
Yu, X., Harris, S. L., & Levine, A. J. (2006) · Cancer Research · doi:10.1158/0008-5472.can-05-4579 · Cited by 688 · see citing works
Gould, S. J., Booth, A. M., & Hildreth, J. E. K. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1831413100 · Cited by 638 · see citing works
Self-citationGeneMANIA Cytoscape plugin: fast gene function predictions on the desktop
Montojo, J., Zuberi, K., Rodriguez, H., Kazi, F. I., Wright, G., Donaldson, S. L., Morris, Q., & Bader, G. D. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btq562 · Cited by 633 · see citing works
Functional association of cyclophilin A with HIV-1 virions
Thali, M., Bukovsky, A., Kondo, E., Rosenwlrth, B., Walsh, C. T., Sodroski, J., & Göttlinger, H. G. (1994) · Nature · doi:10.1038/372363a0 · Cited by 629 · see citing works
Tetraspanin-enriched microdomains: a functional unit in cell plasma membranes
Yáñez‐Mó, M., Barreiro, O., Gordón‐Alonso, M., Sala-Valdés, M., & Sánchez‐Madrid, F. (2009) · Trends in Cell Biology · doi:10.1016/j.tcb.2009.06.004 · Cited by 617 · see citing works
Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids
Salter, R. D., Howell, D. N., & Cresswell, P. (1985) · Immunogenetics · doi:10.1007/bf00375376 · Cited by 573 · see citing works
Chertova, E., Chertov, O., Coren, L. V., Roser, J. D., Trubey, C. M., Bess, J. W., Sowder, R. C., Barsov, E., Hood, B. L., Fisher, R. J., et al. (2006) · Journal of Virology · doi:10.1128/jvi.01013-06 · Cited by 462 · see citing works
Proteomics Analysis of Bladder Cancer Exosomes
Welton, J. L., Khanna, S., Giles, P. J., Brennan, P., Brewis, I. A., Staffurth, J. N., Mason, M. D., & Clayton, A. (2010) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m000063-mcp201 · Cited by 387 · see citing works
A HUPO test sample study reveals common problems in mass spectrometry–based proteomics
HUPO Test Sample Working Group, Bell, A. W., Deutsch, E. W., Au, C. E., Kearney, R. E., Beavis, R., Sechi, S., Nilsson, T., & Bergeron, J. J. M. (2009) · Nature Methods · doi:10.1038/nmeth.1333 · Cited by 351 · see citing works
Discrimination between exosomes and HIV-1: Purification of both vesicles from cell-free supernatants
Cantin, R., Diou, J., Bélanger, D., Tremblay, A., & Gilbert, C. (2008) · Journal of Immunological Methods · doi:10.1016/j.jim.2008.07.007 · Cited by 320 · see citing works
Binding specificity and in vivo targets of the EH domain, a novel protein–protein interaction module
Salcini, A. E., Confalonieri, S., Doria, M., Santolini, E., Tassi, E., Minenkova, O., Cesareni, G., Pelicci, P. G., & Di Fiore, P. P. (1997) · Genes & Development · doi:10.1101/gad.11.17.2239 · Cited by 315 · see citing works
The Proteomics Identifications database: 2010 update
Vizcaíno, J. A., Côté, R. G., Reisinger, F., Barsnes, H., Foster, J. M., Rameseder, J., Hermjakob, H., & Martens, L. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp964 · Cited by 286 · see citing works
Buschow, S. I., van Balkom, B. W. M., Aalberts, M., Heck, A. J. R., Wauben, M. H. M., & Stoorvogel, W. (2010) · Immunology and Cell Biology · doi:10.1038/icb.2010.64 · Cited by 275 · see citing works
Human immunodeficiency virus type 1, human protein interaction database at NCBI
Fu, W., Sanders-Beer, B. E., Katz, K., Maglott, D. R., Pruitt, K. D., & Ptak, R. G. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn708 · Cited by 245 · see citing works
A physical and functional link between cholesterol and tetraspanins
Charrin, S., Manié, S. N., Thiele, C., Billard, M., Gerlier, D., Boucheix, C., & Rubinstein, E. (2003) · European Journal of Immunology · doi:10.1002/eji.200323884 · Cited by 232 · see citing works
CD44 and its Role in Inflammation and Inflammatory Diseases
Johnson, P., & Ruffell, B. (2009) · Inflammation & Allergy - Drug Targets · doi:10.2174/187152809788680994 · Cited by 222 · see citing works
Cytoskeletal proteins inside human immunodeficiency virus type 1 virions
Ott, D. E., Coren, L., Kane, B. P., Busch, L. K., Johnson, D. G., Sowder, R. C., Chertova, E. N., Arthur, L. O., & Henderson, L. E. (1996) · Journal of Virology · doi:10.1128/jvi.70.11.7734-7743.1996 · Cited by 217 · see citing works
Inhibition of HIV-1 gp160-dependent membrane fusion by a furin-directed alpha 1-antitrypsin variant.
Anderson, E. D., Thomas, L., Hayflick, J. S., & Thomas, G. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)74548-7 · Cited by 212 · see citing works
Microvesicles Are a Source of Contaminating Cellular Proteins Found in Purified HIV-1 Preparations
Bess, J. W., Gorelick, R. J., Bosche, W. J., Henderson, L. E., & Arthur, L. O. (1997) · Virology · doi:10.1006/viro.1997.8499 · Cited by 206 · see citing works
Graham, D. R. M., Chertova, E. N., Hilburn, J. M., Arthur, L. O., & Hildreth, J. E. K. (2003) · Journal of Virology · doi:10.1128/jvi.77.15.8237-8248.2003 · Cited by 205 · see citing works
Self-citationLiao, Z., Graham, D. R. M., & Hildreth, J. E. K. (2003) · AIDS Research and Human Retroviruses · doi:10.1089/088922203322280900 · Cited by 185 · see citing works
Self-citationStaubach, S., Razawi, H., & Hanisch, F. (2009) · PROTEOMICS · doi:10.1002/pmic.200800793 · Cited by 175 · see citing works
Esser, M. T., Graham, D. R. M., Coren, L. V., Trubey, C. M., Bess, J. W., Arthur, L. O., Ott, D. E., & Lifson, J. D. (2001) · Journal of Virology · doi:10.1128/jvi.75.13.6173-6182.2001 · Cited by 173 · see citing works
Self-citationHuman cellular restriction factors that target HIV-1 replication
Strebel, K., Luban, J., & Jeang, K.-T. (2009) · BMC Medicine · doi:10.1186/1741-7015-7-48 · Cited by 170 · see citing works
Alpha‐1‐antitrypsin inhibits human immunodeficiency virus type 1
Shapiro, L., Pott, G. B., & Ralston, A. H. (2001) · The FASEB Journal · doi:10.1096/fj.00-0311com · Cited by 163 · see citing works
Gluschankof, P., Mondor, I., Gelderblom, H. R., & Sattentau, Q. J. (1997) · Virology · doi:10.1006/viro.1997.8453 · Cited by 148 · see citing works
Cyclophilin A, TRIM5, and Resistance to Human Immunodeficiency Virus Type 1 Infection
Luban, J. (2007) · Journal of Virology · doi:10.1128/jvi.01519-06 · Cited by 147 · see citing works
Short Communication: Cataloguing the HIV Type 1 Human Protein Interaction Network
Ptak, R., Fu, W., Sanders-Beer, B. E., Dickerson, J. E., Pinney, J., Robertson, D., Rozanov, M., Katz, K., Maglott, D., Pruitt, K., et al. (2008) · AIDS Research and Human Retroviruses · doi:10.1089/aid.2008.0113 · Cited by 142 · see citing works
General Statistical Modeling of Data from Protein Relative Expression Isobaric Tags
Breitwieser, F. P., Müller, A. C., Dayon, L., Köcher, T., Hainard, A., Pichler, P., Schmidt-Erfurth, U. M., Superti‐Furga, G., Sanchez, J., Mechtler, K., et al. (2011) · Journal of Proteome Research · doi:10.1021/pr1012784 · Cited by 127 · see citing works
Actin-Binding Cellular Proteins inside Human Immunodeficiency Virus Type 1
Ott, D. E., Coren, L. V., Johnson, D. G., Kane, B., Sowder, R. C., Kim, Y. D., Fisher, R. J., Zhou, X. Z., Lu, K. P., & Henderson, L. E. (2000) · Virology · doi:10.1006/viro.1999.0075 · Cited by 127 · see citing works
George, M., Ying, G., Rainey, M. A., Solomon, A., Parikh, P. T., Gao, Q., Band, V., & Band, H. (2007) · BMC Cell Biology · doi:10.1186/1471-2121-8-3 · Cited by 115 · see citing works
The ins and outs of syntenin, a multifunctional intracellular adaptor protein
Beekman, J. M., & Coffer, P. J. (2008) · Journal of Cell Science · doi:10.1242/jcs.026401 · Cited by 115 · see citing works
A Role for EHD4 in the Regulation of Early Endosomal Transport
Sharma, M., Naslavsky, N., & Caplan, S. (2008) · Traffic · doi:10.1111/j.1600-0854.2008.00732.x · Cited by 113 · see citing works
Sheng, S., Chen, D., & Eyk, J. E. V. (2005) · Molecular & Cellular Proteomics · doi:10.1074/mcp.t500019-mcp200 · Cited by 110 · see citing works
A role for CD81 on the late steps of HIV-1 replication in a chronically infected T cell line
Grigorov, B., Attuil-Audenis, V., Perugi, F., Nedelec, M., Watson, S., Pique, C., Darlix, J., Conjeaud, H., & Muriaux, D. M. (2009) · Retrovirology · doi:10.1186/1742-4690-6-28 · Cited by 108 · see citing works
Tetraspanins regulate cell-to-cell transmission of HIV-1
Krementsov, D. N., Weng, J., Lambelé, M., Roy, N. H., & Thali, M. (2009) · Retrovirology · doi:10.1186/1742-4690-6-64 · Cited by 102 · see citing works
Ryzhova, E. V., Vos, R. M., Albright, A. V., Harrist, A. V., Harvey, T. S., & González‐Scarano, F. (2006) · Journal of Virology · doi:10.1128/jvi.80.6.2694-2704.2006 · Cited by 96 · see citing works
HIV-host interactions: a map of viral perturbation of the host system.
Pinney, J., Dickerson, J. E., Fu, W., Sanders-Beer, B. E., Ptak, R., & Robertson, D. (2009) · AIDS (London) · doi:10.1097/qad.0b013e328325a495 · Cited by 93 · see citing works
Expanding the Subproteome of the Inner Mitochondria Using Protein Separation Technologies
McDonald, T. G., Sheng, S., Stanley, B. A., Chen, D., Ko, Y. H., Cole, R. N., Pedersen, P. L., & Eyk, J. E. V. (2006) · Molecular & Cellular Proteomics · doi:10.1074/mcp.t500036-mcp200 · Cited by 90 · see citing works
Weng, J., Krementsov, D. N., Khurana, S., Roy, N. H., & Thali, M. (2009) · Journal of Virology · doi:10.1128/jvi.00163-09 · Cited by 89 · see citing works
Gyrase B Inhibitor Impairs HIV-1 Replication by Targeting Hsp90 and the Capsid Protein
Vozzolo, L., Loh, B., Gane, P. J., Tribak, M., Zhou, L., Anderson, I., Nyakatura, E. K., Jenner, R. G., Selwood, D. L., & Fassati, A. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.155275 · Cited by 82 · see citing works
HIV Acquires Functional Adhesion Receptors from Host Cells
GUO, M. M. L., & Hildreth, J. E. K. (1995) · AIDS Research and Human Retroviruses · doi:10.1089/aid.1995.11.1007 · Cited by 68 · see citing works
Self-citationQuantitative Proteomics Analysis of Human Endothelial Cell Membrane Rafts
Guo, Y., Singleton, P. A., Rowshan, A., Guček, M., Cole, R. N., Graham, D. R. M., Eyk, J. E. V., & Garcia, J. G. N. (2007) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m600398-mcp200 · Cited by 68 · see citing works
Self-citationLiao, Z., Roos, J. W., & Hildreth, J. E. K. (2000) · AIDS Research and Human Retroviruses · doi:10.1089/088922200309232 · Cited by 67 · see citing works
Self-citationTrubey, C. M., Chertova, E. N., Coren, L. V., Hilburn, J. M., Hixson, C. V., Nagashima, K., Lifson, J. D., & Ott, D. E. (2003) · Journal of Virology · doi:10.1128/jvi.77.23.12699-12709.2003 · Cited by 64 · see citing works
Hioe, C. E., Bastiani, L., Hildreth, J. E. K., et al. (1998) · PubMed · Cited by 62 · see citing works
Self-citationEdwards, N. J., Wu, X., & Tseng, C. (2009) · Clinical Proteomics · doi:10.1007/s12014-009-9024-5 · Cited by 51 · see citing works
Self-citationOtt, D. E. (2008) · Methods in molecular biology · doi:10.1007/978-1-59745-170-3_2 · Cited by 49 · see citing works
Harrist, A. V., Ryzhova, E. V., Harvey, T. S., & González‐Scarano, F. (2009) · PLoS ONE · doi:10.1371/journal.pone.0005020 · Cited by 38 · see citing works
Parish, L. A., Colquhoun, D. R., Ubaida‐Mohien, C., et al. (2011) · Journal of Proteome Research · doi:10.1021/pr2006268 · Cited by 37 · see citing works
Self-citationRai, T., Mosoian, A., & Resh, M. D. (2010) · Journal of Virology · doi:10.1128/jvi.01584-09 · Cited by 34 · see citing works
HIV-induced loss of CD44 expression in monocytic cell lines.
GUO, M. M. L., & Hildreth, J. E. K. (1993) · The Journal of Immunology · doi:10.4049/jimmunol.151.4.2225 · Cited by 30 · see citing works
Self-citationQuantitative proteomic analysis of lentiviral vectors using 2‐DE
Denard, J., Rundwasser, S., Laroudie, N., Gonnet, F., Naldini, L., Radrizzani, M., Galy, A. H. M., Merten, O., Danos, O., & Svinartchouk, F. (2009) · PROTEOMICS · doi:10.1002/pmic.200800747 · Cited by 30 · see citing works
Ubaida‐Mohien, C., Colquhoun, D. R., Mathias, D. K., Gibbons, J. G., Armistead, J. S., Rodriguez, M. C., Rodriguez, M. H., Edwards, N. J., Hartler, J., Thallinger, G., et al. (2012) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m112.019596 · Cited by 28 · see citing works
Self-citationCD48 Delivers an Accessory Signal for CD40-Mediated Activation of Human B Cells
Klyushnenkova, E., Li, L., Armitage, R. J., et al. (1996) · Cellular Immunology · doi:10.1006/cimm.1996.0297 · Cited by 26 · see citing works
Impaired Infectivity of Ritonavir-resistant HIV Is Rescued by Heat Shock Protein 90AB1
Joshi, P., & Stoddart, C. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.248021 · Cited by 23 · see citing works
MASPECTRAS 2: An integration and analysis platform for proteomic data
Ubaida‐Mohien, C., Hartler, J., Breitwieser, F. P., et al. (2010) · PROTEOMICS · doi:10.1002/pmic.201000075 · Cited by 22 · see citing works
Self-citationCordelier, P., & Strayer, D. S. (2003) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/s0925-4439(03)00084-x · Cited by 20 · see citing works
Graham, D. R. M., Mitsak, M., Elliott, S. T., Chen, D., Whelan, S. A., Hart, G. W., & Eyk, J. E. V. (2008) · PROTEOMICS · doi:10.1002/pmic.200800608 · Cited by 20 · see citing works
Self-citationABRF-PRG07: advanced quantitative proteomics study.
Falick, A. M., Lane, W. S., Lilley, K. S., et al. (2011) · PubMed · Cited by 13 · see citing works
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Greco, T. M., Diner, B. A., & Cristea, I. M. (2014) · Annual Review of Virology · doi:10.1146/annurev-virology-031413-085527 · Cited by 67 · see citing works
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- Authors
- Linde, M. E., Colquhoun, D. R., Ubaida Mohien, C., Kole, T., Aquino, V., Cotter, R., Edwards, N., Hildreth, J. E. K., & Graham, D. R.
- Affiliations
- Johns Hopkins University (US)
- Georgetown University (US)
- University of California, Davis (US)
- Corresponding author
- Graham, David R.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-03-29
- Volume
- 12
- Issue
- 5
- Pages
- 2045-2054
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- PMC
- PMC4065613
- OpenAlex
- W2115817524
- Cited by
- 60
- References
- 66
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 4 · 2022: 2 · 2021: 1 · 2020: 4 · 2019: 6 · 2018: 7 · 2017: 7 · 2016: 5 · 2015: 5 · 2014: 11 · 2013: 2
- FWCI
- 3.51
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Proteome Research (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Johns Hopkins University
- National Institutes of Health
- National Heart, Lung, and Blood Institute
- National Institute of Mental Health (P01MH070306)
- National Cancer Institute
- National Institute of Allergy and Infectious Diseases (T32AI007247)
- National Institute of Neurological Disorders and Stroke (R01NS039253)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
- Keywords
- HIV-1, virus purification, density modification, cholesterol, microvesicles, CD4+ T cells, monocyte-derived macrophages, actin isoforms, molecular chaperones, ERM proteins, cyclophilin A, host-virus interaction
- MeSH terms
- Cell Fractionation, Cell Line, Centrifugation, Density Gradient, Cholesterol, Humans, Subcellular Fractions, Virion, CD4-Positive T-Lymphocytes, HIV-1, Cluster Analysis, Proteome, Tandem Mass Spectrometry, Host-Pathogen Interactions
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