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Cited by 27Muscle Physiology and DisordersThyroid Disorders and TreatmentsCardiomyopathy and Myosin Studies

Contractility and myosin isoform compositions of skeletal muscles and muscle cells from rats treated with thyroid hormone for 0, 4 and 8 weeks

LI, XIAOPENG, LARSSON, LARS·1997·Journal of Muscle Research & Cell Motility
DOI 10.1023/a:1018674126229
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Abstract

The effects of 4 and 8 weeks of thyroid hormone (3,5,3'-triiodothyronine, T3) treatment on skeletal muscles of young (3-6 months) male Wistar rats were investigated in the present study. In the slow-twitch soleus, contraction and half-relaxation times of the isometric twitch were significantly shorter in hyperthyroid rats than in the control group, and twitch duration was shorter in rats treated with T3 for 8 weeks than for 4 weeks. All single soleus muscle fibres from hyperthyroid rats co-expressed types I and IIA myosin heavy chains (type I/IIA fibres) or type I, IIA and IIX myosin heavy chains (type I/IIAX fibres), while only type I MyHC fibres were isolated from the controls. A significantly higher content of type IIA myosin heavy chain and fast myosin light chain isoforms was observed in soleus fibres from the 8-week than from 4-week T3 group. There was no significant difference in maximum velocity of unloaded shortening (V0) between type I myosin heavy chain fibres from controls (1.12 +/- 0.46 muscle lengths s-1, n = 48) and type I/IIA myosin heavy chain fibres from the 4-1.09 +/- 0.36 muscle length s-1, n = 33) and 8-week (1.03 +/- 0.31 muscle lengths s(-1), n = 31) groups, but type I/IIAX fibres from the 8-week T3 group had significantly higher V0 (1.56 +/- 0.10, n = 5) than type I from control and type I/IIA from hyperthyroid rats. In the fast-twitch extensor digitorum longus, neither myosin isoform composition, twitch duration nor V0 was affected by 4 or 8 weeks of T3 exposure. In conclusion, a dramatic and exposure duration-dependent change in the contractile speed of the isometric twitch and the expression of fast myosin isoforms was observed in soleus, but not in extensor digitorum longus, in response to T3 treatment. Long-term T3 treatment had relatively less influence, however, on V0 at the single cell level in spite of the dramatic increase in fast myosin isoforms.

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Crossref · 10.1023/a:1018674126229

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  • Four weeks of T3 treatment induces an increase in type-IIA fibers and a downregulation of type-I fibers in male and female rat soleus muscle.

    “Four weeks of T3 treatment induces an increase in type-IIA fibers and a downregulation of type-I fibers in male and female rat soleus muscle (57, 101).”

    From Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition (2015) · cites this paper as [57]

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Authors
LI, X., & LARSSON, L.
Affiliations
Karolinska University Hospital (SE)
ORCID
LARSSON, LARS
Venue
Journal of Muscle Research & Cell Motility
Publisher
Springer Science and Business Media LLC
Published
1997-05
Volume
18
Issue
3
Pages
335-344
ISSN
  • 0142-4319 (ISSN-L)
  • 1573-2657
DOI
10.1023/a:1018674126229
OpenAlex
W229600376
Cited by
27
References
35
Citations by year
2023: 1 · 2017: 1 · 2015: 1 · 2014: 1
FWCI
1.19
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Journal of Muscle Research and Cell Motility (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Wenner-Gren Foundation
  • Vetenskapsrådet
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiomyopathy and Myosin Studies
Keywords
thyroid hormones, triiodothyronine, hyperthyroidism, skeletal muscle contractility, myosin heavy chain isoforms, myosin light chain isoforms, soleus muscle, extensor digitorum longus, isometric twitch, twitch duration, unloaded shortening velocity, muscle fiber type, type I muscle fibers, fast-twitch fibres
MeSH terms
Animals, Body Weight, Male, Muscle Contraction, Organ Size, Time Factors, Triiodothyronine, Rats, Wistar, Muscle, Skeletal, Muscle Fibers, Fast-Twitch, Muscle Fibers, Slow-Twitch, Myosin Heavy Chains, Rats

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