Mast Cell Degranulation Induces Delayed Rectal Allodynia in Rats: Role of Histamine and 5-HT
Abstract
Visceral hypersensitivity is a common feature of functional bowel disorders, where an increased number of mast cells have often been described. Thus, we investigated the effect of an experimental mast cell degranulation induced by BrX-537A on somatic (tail heating) and visceral (rectal distension) sensitivity in rats and the involvement of histamine and/or serotonin on this last response. After BrX-537A administration, the latency of tail withdrawal reflex was shortened within the 2- to 8-hr period. Moreover, BrX-537A reduced the distension volume threshold from 0.8 ml to 0.4 ml inducing allodynia, from 6 to 12 hr after its administration. This effect was suppressed by doxantrazole (mast cell stabilizing agent) and WAY 100635 (5-HT1A receptor antagonist), and reproduced by 5-HTP (5-HT precursor) and 8-OH-DPAT (5-HT1A receptor agonist). However, neither granisetron (5-HT3 receptor antagonist) nor H1, H2, or H3 histamine receptor antagonists modified the BrX-537A-induced allodynia. Consequently, mast cell degranulation initiates a delayed somatic and visceral allodynia, with the participation of serotonin, through 5-HT1A receptor activation, on the visceral response.
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Activation of the 5-hydroxytryptamine receptor 1A, rather than histamine receptors, has been implicated in complex abdominal pain mediated by mast cell degranulation.
“Furthermore, the activation of the 5-hydroxytryptamine receptor 1A (5-HT1A), rather than the histamine receptors, has also been implicated in the complex abdominal pain mediated by mast cell degranulation (82).”
From Nociplastic pain: the prominent role of non-neuronal cells in central and peripheral sensitization (2026) · cites this paper as [82]
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Functional Abdominal Pain: The Basic Science
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Cellular Neurophysiology of Enteric Neurons
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Tumor bearing decreases systemic acute inflammation in rats – role of mast cell degranulation
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Serotonergic Integration In the Intestinal Mucosa
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Feng, B., Schwartz, E. S., & Gebhart, G. F. (2012)
Wood, J. D. (2004)
Minushkin, O. N., Khlynova, O. V., Sitkin, S. I., Yakovenko, É. P., Kravchuk, Y. A., Seliverstov, P. V., & Александровна, Б. Е. (2024) · Almanac of Clinical Medicine · doi:10.18786/2072-0505-2024-52-023
- Authors
- Coelho, A.-M., Fioramonti, J., & Bueno, L.
- Affiliations
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Venue
- Digestive Diseases and Sciences
- Published
- 1998-04
- Volume
- 43
- Issue
- 4
- Pages
- 727-737
- ISSN
- 0163-2116 (ISSN-L)
- 1573-2568
- OpenAlex
- W1588618357
- Cited by
- 86
- References
- 48
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 2 · 2014: 4 · 2013: 1 · 2012: 5
- FWCI
- 3.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Digestive Diseases and Sciences (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Keywords
- mast cell degranulation, visceral hypersensitivity, histamine, serotonin, 5-HT1A receptor, doxantrazole, tail withdrawal reflex, functional bowel disorders, 5-hydroxytryptophan, 8-OH-DPAT
- MeSH terms
- Animals, Histamine, Ionophores, Lasalocid, Male, Mast Cells, Receptors, Serotonin, Rectum, Serotonin, Skin Physiological Phenomena, Time Factors, Cell Degranulation, Rats, Wistar, Pain Threshold, Receptors, Serotonin, 5-HT1, Rats
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