Short-term caloric restriction and regulatory proteins of apoptosis in heart, skeletal muscle and kidney of Fischer 344 rats
Abstract
Long-term caloric restriction reduces oxidative stress, increases mean and maximum lifespan in rodents and tends to enhance apoptosis, particularly in the liver. We investigated the effect of short-term (2 months) caloric restriction (40% reduction) in 6-month-old male Fischer 344 rats on various indicators of apoptosis (caspase-3, -7, -12, the inhibitor of apoptosis protein XIAP and cytoplasmic histone-associated DNA fragments) in the post-mitotic heart and gastrocnemius muscle, and the kidney that contains mitotic cells. Short-term caloric restriction significantly reduced body mass (30%), gastrocnemius muscle mass (22%), heart mass (25%) and kidney mass (32%) compared to ad libitum controls. The levels of procaspase-3 in gastrocnemius muscle and caspase-3 in kidney were significantly lower in the caloric restricted than in the ad libitum fed group. While caloric restriction did not alter DNA fragmentation levels (indicative of apoptosis), differences did exist amongst tissues with significantly elevated levels of fragmentation in the kidney compared to the heart and gastrocnemius muscle and significantly higher levels in the heart compared to gastrocnemius muscle. No differences were observed between groups in the levels of procaspase-7 or -12 or in XIAP (an endogenous inhibitor of apoptosis, particularly of caspase-3 and -7) in any tissue. The active forms of caspase-7 and -12 were present only in the kidney. These findings suggest that while the rate of apoptosis was hig…
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The accumulation of mitochondrial dysfunction contributes to sarcopenia.
“the accumulation of mitochondrial dysfunction has been shown to contribute to sarcopenia (Trounce et al. 1989; Selman et al. 2003; Dirks & Leeuwenburgh, 2004; Leeuwenburgh et al. 2005; Short et al. 2005; Chabi et al. 2008; Marzetti et al. 2008; Gouspillou et al. 2010; Picard et al. 2010; Picard et al. 2011; Lanza et al. 2012; Umanskaya et al. 2014; Gouspillou et al. 2014a; Gouspillou et al. 2014b)”
From Parkin overexpression protects from ageing‐related loss of muscle mass and strength (2019) · cites this paper as Selman et al. 2003
Aged skeletal muscles display increased mitochondrial-mediated apoptosis.
“Notably, aged skeletal muscles display impaired mitochondrial energetics (Trounce et al. 1989; Short et al. 2005; Gouspillou et al. 2010; Lanza et al. 2012; Gouspillou et al. 2014a) and increased mitochondrial‐mediated apoptosis (Selman et al. 2003; Dirks & Leeuwenburgh, 2004; Leeuwenburgh et al. 2005; Chabi et al. 2008; Marzetti et al. 2008; Picard et al. 2010; Picard et al. 2011; Gouspillou et a”
From Parkin overexpression protects from ageing‐related loss of muscle mass and strength (2019) · cites this paper as Selman et al. 2003
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ResultMitochondrial morphology is altered in atrophied skeletal muscle of aged mice
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- Authors
- Selman, C., Gredilla, R., Phaneuf, S., Kendaiah, S., Barja, G., & Leeuwenburgh, C.
- Affiliations
- University of Florida Health (US)
- Universidad Complutense de Madrid (ES)
- Corresponding author
- Leeuwenburgh, Christiaan
- Venue
- Biogerontology
- Published
- 2003-05
- Volume
- 4
- Issue
- 3
- Pages
- 141-147
- ISSN
- 1389-5729 (ISSN-L)
- 1573-6768
- OpenAlex
- W2134090073
- Cited by
- 24
- References
- 44
- Citations by year
- 2021: 3 · 2020: 1 · 2019: 2 · 2015: 1 · 2013: 1 · 2012: 1
- FWCI
- 0.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Biogerontology (publishedVersion)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- Funders
- National Institutes of Health
- National Institute on Aging (AG17994-01, R01AG017994)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Health Sciences › Medicine › Physiology › Dietary Effects on Health
- Keywords
- caloric restriction, apoptosis, caspase-3, caspase-7, caspase-12, XIAP, heart, skeletal muscle, kidney, body mass reduction
- MeSH terms
- Aging, Animals, Body Weight, Calpain, Kidney, Male, Myocardium, Nucleosomes, Organ Size, Proteins, Rats, Inbred F344, Apoptosis, Muscle, Skeletal, Caspases, Caloric Restriction, Rats, X-Linked Inhibitor of Apoptosis Protein, Caspase 3, Caspase 7, Caspase 12
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