Affinity Selection and Mass Spectrometry-Based Strategies to Identify Lead Compounds in Combinatorial Libraries
Abstract
The screening of diverse libraries of small molecules created by combinatorial synthetic methods is a recent development which has the potential to accelerate the identification of lead compounds in drug discovery. We have developed a direct and rapid method to identify lead compounds in libraries involving affinity selection and mass spectrometry. In our strategy, the receptor or target molecule of interest is used to isolate the active components from the library physically, followed by direct structural identification of the active compounds bound to the target molecule by mass spectrometry. In a drug design strategy, structurally diverse libraries can be used for the initial identification of lead compounds. Once lead compounds have been identified, libraries containing compounds chemically similar to the lead compound can be generated and used to optimize the binding characteristics. These strategies have also been adopted for more detailed studies of protein-ligand interactions.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Native MS detects ligands directly bound to a protein, whereas affinity MS approaches typically measure ligand binding indirectly as free compounds.
“An important difference between native MS and affinity MS is that native MS detects ligands directly bound to a protein, whereas affinity MS approaches typically measure ligand binding indirectly as free compounds.”
From Native metabolomics identifies the rivulariapeptolide family of protease inhibitors (2022) · cites this paper as [25]
Cites
Electrospray Ionization for Mass Spectrometry of Large Biomolecules
Fenn, J. B., Mann, M., Meng, C. K., Wong, S. F., & Whitehouse, C. M. (1989) · Science · doi:10.1126/science.2675315 · Cited by 7,634 · see citing works
Synthesis and Applications of Small Molecule Libraries
Thompson, L. A., & Ellman, J. A. (1996) · Chemical Reviews · doi:10.1021/cr9402081 · Cited by 1,456 · see citing works
Zuckermann, R. N., Kerr, J. M., Kent, S. B. H., & Moos, W. H. (1992) · Journal of the American Chemical Society · doi:10.1021/ja00052a076 · Cited by 1,279 · see citing works
General method for rapid synthesis of multicomponent peptide mixtures
Furka, Á., Sebestyén, F., ASGEDOM, M., & Dibó, G. (1991) · International journal of peptide & protein research · doi:10.1111/j.1399-3011.1991.tb00765.x · Cited by 1,155 · see citing works
Gordon, E. M., Barrett, R. W., Dower, W. J., Fodor, S. P. A., & Gallop, M. A. (1994) · Journal of Medicinal Chemistry · doi:10.1021/jm00036a001 · Cited by 1,142 · see citing works
Gallop, M. A., Barrett, R. W., Dower, W. J., Fodor, S. P. A., & Gordon, E. M. (1994) · Journal of Medicinal Chemistry · doi:10.1021/jm00035a001 · Cited by 1,135 · see citing works
New developments in biochemical mass spectrometry: electrospray ionization.
Smith, R. D., Loo, J., Edmonds, C. G., Barinaga, C., & Udseth, H. (1990) · Analytical Chemistry · doi:10.1021/ac00208a002 · Cited by 975 · see citing works
Peptoids: a modular approach to drug discovery.
Simon, R. J., Kania, R. S., Zuckermann, R. N., Huebner, V. D., Jewell, D. A., Banville, S. C., Ng, S., Wang, L., Rosenberg, S., & Marlowe, C. K. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.20.9367 · Cited by 956 · see citing works
Self-citationCombinatorial synthesis — the design of compound libraries and their application to drug discovery
Terrett, N. K., Gardner, M., Gordon, D. W., Kobylecki, R. J., & Steele, J. (1995) · Tetrahedron · doi:10.1016/0040-4020(95)00467-m · Cited by 660 · see citing works
Measuring Diversity: Experimental Design of Combinatorial Libraries for Drug Discovery
Martin, É., Blaney, J. M., Siani, M. A., Spellmeyer, D. C., Wong, A. K., & Moos, W. H. (1995) · Journal of Medicinal Chemistry · doi:10.1021/jm00009a003 · Cited by 298 · see citing works
Zuckermann, R. N., Kerr, J. M., Siani, M. A., Banville, S. C., & Santi, D. V. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.10.4505 · Cited by 109 · see citing works
Till, J. H., Annan, R. S., Carr, S. A., & Miller, W. T. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)37302-7 · Cited by 77 · see citing works
Recent advances in the generation of chemical diversity libraries
Desai, M. C., Zuckermann, R. N., & Moos, W. H. (1994) · Drug Development Research · doi:10.1002/ddr.430330214 · Cited by 60 · see citing works
Petithory, J. R., Masiarz, F. R., Kirsch, J. F., Santi, D. V., & Malcolm, B. A. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.24.11510 · Cited by 46 · see citing works
Flagged first, then by citations
1 of the 15 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Tethering: Fragment-Based Drug Discovery
Erlanson, D. A., Wells, J. A., & Braisted, A. C. (2004) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.33.110502.140409 · Cited by 385 · see citing works
Mixture-Based Synthetic Combinatorial Libraries
Houghten, R. A., Pinilla, C., Appel, J. R., Blondelle, S. E., Dooley, C. T., Eichler, J., Nefzi, A., & Ostresh, J. M. (1999) · Journal of Medicinal Chemistry · doi:10.1021/jm990174v · Cited by 257 · see citing works
Zuckermann, R. N. (2010) · Biopolymers · doi:10.1002/bip.21573 · Cited by 241 · see citing works
LC/MS applications in drug development.
Lee, M. S., & Kerns, E. H. (1999) · PubMed · doi:10.1002/(sici)1098-2787(1999)18:3/4 · Cited by 207 · see citing works
Receptor–ligand binding assays: Technologies and Applications
de Jong, L. A. A., Uges, D. R. A., Franke, J. P., & Bischoff, R. (2005) · Journal of Chromatography B · doi:10.1016/j.jchromb.2005.10.002 · Cited by 205 · see citing works
Ultra-large chemical libraries for the discovery of high-affinity peptide binders
Quartararo, A. J., Gates, Z. P., Somsen, B. A., Hartrampf, N., Ye, X., Shimada, A., Kajihara, Y., Ottmann, C., & Pentelute, B. L. (2020) · Nature Communications · doi:10.1038/s41467-020-16920-3 · Cited by 154 · see citing works
High-Throughput Solution-Based Medicinal Library Screening against Human Serum Albumin
Flarakos, J., Morand, K. L., & Vouros, P. (2005) · Analytical Chemistry · doi:10.1021/ac048685z · Cited by 137 · see citing works
Cannabinoids Block Cellular Entry of SARS-CoV-2 and the Emerging Variants
van Breemen, R. B., Muchiri, R. N., Bates, T. A., Weinstein, J. B., Leier, H. C., Farley, S., & Tafesse, F. G. (2022) · Journal of Natural Products · doi:10.1021/acs.jnatprod.1c00946 · Cited by 135 · see citing works
Annis, D. A., Nazef, N., Chuang, C., Scott, M. P., & Nash, H. M. (2004) · Journal of the American Chemical Society · doi:10.1021/ja048365x · Cited by 130 · see citing works
Combinatorial Chemistry and Mass Spectrometry in the 21st Century Drug Discovery Laboratory
Kassel, D. B. (2001) · Chemical Reviews · doi:10.1021/cr990085q · Cited by 114 · see citing works
Muckenschnabel, I., Falchetto, R. A., Mayr, L. M., & Filipuzzi, I. (2003) · Analytical Biochemistry · doi:10.1016/j.ab.2003.09.040 · Cited by 105 · see citing works
Mass Spectrometry Innovations in Drug Discovery and Development
Papac, D. I., & Shahrokh, Z. (2001) · Pharmaceutical Research · doi:10.1023/a:1011049231231 · Cited by 104 · see citing works
Annis, D. A., Athanasopoulos, J., Curran, P. J., Felsch, J. S., Kalghatgi, K., Lee, W. H., Nash, H. M., Orminati, J.-P. A., Rosner, K. E., Shipps, G. W., et al. (2004) · International Journal of Mass Spectrometry · doi:10.1016/j.ijms.2003.11.022 · Cited by 100 · see citing works
Chemical ligands, genomics and drug discovery
Lenz, G. R., Nash, H. M., & Jindal, S. (2000) · Drug Discovery Today · doi:10.1016/s1359-6446(00)01468-9 · Cited by 85 · see citing works
Biochemical applications of mass spectrometry in pharmaceutical drug discovery
Geoghegan, K. F., & Kelly, M. A. (2004) · Mass Spectrometry Reviews · doi:10.1002/mas.20019 · Cited by 77 · see citing works
Moy, F. J., Haraki, K., Mobilio, D., Walker, G., Powers, R., Tabei, K., Tong, H., & Siegel, M. M. (2001) · Analytical Chemistry · doi:10.1021/ac0006270 · Cited by 66 · see citing works
Impact of mass spectrometry on combinatorial chemistry
Süßmuth, R. D., & Jung, G. (1999) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/s0378-4347(98)00513-1 · Cited by 64 · see citing works
Mass spectrometry and immobilized enzymes for the screening of inhibitor libraries
Cancilla, M. T., Leavell, M. D., Chow, J., & Leary, J. A. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.220403997 · Cited by 61 · see citing works
Tao, Y., Zhang, Y., Wáng, Y., & Cheng, Y. (2013) · Analytica Chimica Acta · doi:10.1016/j.aca.2013.04.058 · Cited by 61 · see citing works
Lim, H., Stellingweif, S., Sisenwine, S. F., & Chan, K.-K. (1999) · Journal of Chromatography A · doi:10.1016/s0021-9673(98)00956-x · Cited by 60 · see citing works
Biomedical and Biological Mass Spectrometry
Mano, N., & Goto, J. (2003) · Analytical Sciences · doi:10.2116/analsci.19.3 · Cited by 59 · see citing works
Kinetics and performance of a co-immobilised system of amyloglucosidase andZymomonas mobilis
Hellendoorn, L., Ottengraf, S. P. P., van den Heuvel, J. C., Pennings, J. A. M. M., dos Santos, V. A. P. M., & Wijffels, R. H. (1999) · Biotechnology and Bioengineering · doi:10.1002/(sici)1097-0290(19990620)63:6 · Cited by 6 · see citing works
Blom, K. F., Larsen, B. S., & McEwen, C. N. (1998) · Journal of Combinatorial Chemistry · doi:10.1021/cc980010k · Cited by 57 · see citing works
Tao, Y., Chen, Z., Zhang, Y., Wáng, Y., & Cheng, Y. (2013) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2013.02.024 · Cited by 54 · see citing works
Analysis and screening of combinatorial libraries using mass spectrometry
Shin, Y. G., & van Breemen, R. B. (2001) · Biopharmaceutics & Drug Disposition · doi:10.1002/bdd.278 · Cited by 50 · see citing works
Foley, T. L., Burchett, W., Chen, Q., Flanagan, M. E., Kapinos, B., Li, X.-Y., Montgomery, J. I., Ratnayake, A. S., Zhu, H., & Peakman, M. (2021) · SLAS DISCOVERY · doi:10.1177/2472555220979589 · Cited by 49 · see citing works
Whitehurst, C. E., Nazef, N., Annis, D. A., Hou, Y., Murphy, D. M., Spacciapoli, P., Yao, Z., Ziebell, M. R., Cheng, C. C., Shipps, G. W., et al. (2006) · SLAS DISCOVERY · doi:10.1177/1087057105284340 · Cited by 49 · see citing works
Native metabolomics identifies the rivulariapeptolide family of protease inhibitors
Reher, R., Aron, A. T., Fajtová, P., Stincone, P., Wagner, B., Pérez-Lorente, A. I., Liu, C., Shalom, I. Y. B., Bittremieux, W., Wang, M., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-32016-6 · Cited by 53 · see citing works
Mass spectrometry and combinatorial chemistry: a short outline
Triolo, A., Altamura, M., Cardinali, F., Sisto, A., & Maggi, C. A. (2001) · Journal of Mass Spectrometry · doi:10.1002/jms.238 · Cited by 46 · see citing works
Lyubarskaya, Y. V., Carr, S. A., Dunnington, D. J., Prichett, W. P., Fisher, S. M., Appelbaum, E. R., Jones, C. S., & Karger, B. L. (1998) · Analytical Chemistry · doi:10.1021/ac980330q · Cited by 45 · see citing works
Affinity selection mass spectrometry speeding drug discovery
Prudent, R., Lemoine, H., Walsh, J. J., & Roche, D. (2023) · Drug Discovery Today · doi:10.1016/j.drudis.2023.103760 · Cited by 43 · see citing works
Su, W., Ge, R., Ding, D., Chen, W., Wang, W., Yan, H., Wang, W., Yuan, Y., Liu, H., Zhang, M., et al. (2021) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.1c00170 · Cited by 43 · see citing works
Strategic Use of Affinity-Based Mass Spectrometry Techniques in the Drug Discovery Process
Kelly, M. A., McLellan, T. J., & Rosner, P. J. (2001) · Analytical Chemistry · doi:10.1021/ac010569y · Cited by 42 · see citing works
Muchiri, R. N., & van Breemen, R. B. (2020) · Journal of Mass Spectrometry · doi:10.1002/jms.4647 · Cited by 41 · see citing works
Library Design-Facilitated High-Throughput Sequencing of Synthetic Peptide Libraries
Vinogradov, A. A., Gates, Z. P., Zhang, C., Quartararo, A. J., Halloran, K. H., & Pentelute, B. L. (2017) · ACS Combinatorial Science · doi:10.1021/acscombsci.7b00109 · Cited by 40 · see citing works
Competitive Binding Assays Made Easy with a Native Marker and Mass Spectrometric Quantification
Höfner, G. C., & Wanner, K. T. (2003) · Angewandte Chemie International Edition · doi:10.1002/anie.200351806 · Cited by 40 · see citing works
Continuous-Flow, On-Line Monitoring of Biospecific Interactions Using Electrospray Mass Spectrometry
Hogenboom, A. C., de Boer, A. R., Derks, R. J. E., & Irth, H. (2001) · Analytical Chemistry · doi:10.1021/ac010026o · Cited by 37 · see citing works
Methods of screening combinatorial libraries using immobilized or restrained receptors
Woodbury, C. P., & Venton, D. L. (1999) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/s0378-4347(99)00006-7 · Cited by 33 · see citing works
Annis, A., Chuang, C., & Nazef, N. (2007) · Methods and principles in medicinal chemistry · doi:10.1002/9783527610907.ch3 · Cited by 32 · see citing works
Generating and analyzing combinatorial chemistry libraries
Wentworth, P., & Janda, K. D. (1998) · Current Opinion in Biotechnology · doi:10.1016/s0958-1669(98)80093-1 · Cited by 31 · see citing works
Automated affinity selection for rapid discovery of peptide binders
Zhang, G., Li, C., Quartararo, A. J., Loas, A., & Pentelute, B. L. (2021) · Chemical Science · doi:10.1039/d1sc02587b · Cited by 30 · see citing works
Hsieh, F., Keshishian, H., & Muir, C. (1998) · SLAS DISCOVERY · doi:10.1177/108705719800300305 · Cited by 28 · see citing works
Adam, G. C., Parish, C. A., Wisniewski, D., Meng, J., Liu, M., Calati, K., Stein, B. D., Athanasopoulos, J., Liberator, P., Roemer, T., et al. (2008) · Journal of the American Chemical Society · doi:10.1021/ja805531w · Cited by 27 · see citing works
Analytical tools for the detection and characterization of biologically active compounds from nature
van Elswijk, D. A., & Irth, H. (2002) · Phytochemistry Reviews · doi:10.1023/a:1026089809218 · Cited by 26 · see citing works
Drug discovery from natural products using affinity selection-mass spectrometry
Muchiri, R. N., & van Breemen, R. B. (2021) · Drug Discovery Today Technologies · doi:10.1016/j.ddtec.2021.10.005 · Cited by 25 · see citing works
Unravelling seemingly complex chemistry of reactions using electrospray ionization mass spectrometry
Bakhtiar, R., & Hop, C. E. C. A. (1999) · Journal of Physical Organic Chemistry · doi:10.1002/(sici)1099-1395(199907)12:7 · Cited by 23 · see citing works
Sharma, J. P., Besanger, T. R., & Brennan, J. D. (2008) · Analytical Chemistry · doi:10.1021/ac702421e · Cited by 19 · see citing works
Kinetic capillary electrophoresis in screening oligonucleotide libraries for protein binders
Le, A. T. H., Krylova, S. M., & Krylov, S. N. (2023) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2023.117061 · Cited by 18 · see citing works
Screening combinatorial libraries
Venton, D. L., & Woodbury, C. P. (1999) · Chemometrics and Intelligent Laboratory Systems · doi:10.1016/s0169-7439(99)00009-x · Cited by 18 · see citing works
Zhang, G., Brown, J. S., Quartararo, A. J., Li, C., Tan, X., Hanna, S., Antilla, S., Cowfer, A. E., Loas, A., & Pentelute, B. L. (2022) · Communications Chemistry · doi:10.1038/s42004-022-00625-3 · Cited by 16 · see citing works
Höfner, G. C., & Wanner, K. T. (2003) · Angewandte Chemie · doi:10.1002/ange.200351806 · Cited by 15 · see citing works
Translating Senotherapeutic Interventions into the Clinic with Emerging Proteomic Technologies
Dey, A. K., Banarjee, R., Boroumand, M., Rutherford, D. V., Strassheim, Q., Nyunt, T., Olinger, B., & Basisty, N. (2023) · Biology · doi:10.3390/biology12101301 · Cited by 12 · see citing works
Affinity-based ranking of ligands for DPP-4 from mixtures
Adam, G. C., Meng, J., Athanasopoulos, J., Zhang, X., & Chapman, K. T. (2007) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2007.02.040 · Cited by 13 · see citing works
Davis, R. G., Anderegg, R. J., & Blanchard, S. G. (1999) · Tetrahedron · doi:10.1016/s0040-4020(99)00685-7 · Cited by 13 · see citing works
Soricellis, G. D., Rinaldi, F., Tengattini, S., Temporini, C., Negri, S., Capelli, D., Montanari, R., Cena, H., Salerno, S., Massolini, G., et al. (2024) · Analytica Chimica Acta · doi:10.1016/j.aca.2024.342666 · Cited by 12 · see citing works
Mass spectrometry–based bioassay for the screening of soluble orphan receptors
van Elswijk, D. A., Tjaden, U. R., van der Greef, J., & Irth, H. (2001) · International Journal of Mass Spectrometry · doi:10.1016/s1387-3806(01)00445-6 · Cited by 11 · see citing works
Avital‐Shmilovici, M., Liu, X., Shaler, T. A., Lowenthal, A. C., Bourbon, P., Snider, J., Tambo-ong, A., Repellin, C. E., Yniguez, K., Sambucetti, L. C., et al. (2022) · ACS Central Science · doi:10.1021/acscentsci.1c01041 · Cited by 10 · see citing works
The Intersection of Strategy and Drug Research
Moos, W. H. (2007) · Elsevier eBooks · doi:10.1016/b0-08-045044-x/00033-x · Cited by 10 · see citing works
Gu, Y., Liu, M., & Quinn, R. J. (2022) · Frontiers in Analytical Science · doi:10.3389/frans.2022.1014017 · Cited by 10 · see citing works
Applications of mass spectrometry to signal transduction
Resing, K. A., & Ahn, N. G. (1999) · Progress in Biophysics and Molecular Biology · doi:10.1016/s0079-6107(98)00048-0 · Cited by 9 · see citing works
Driving Affinity Selection by Centrifugal Force
Harlan, J. E., Egan, D. A., Ladror, U. S., Snyder, S. W., Tang, M., BUKO, A. M., & Holzman, T. F. (2003) · Assay and Drug Development Technologies · doi:10.1089/154065803322302763 · Cited by 8 · see citing works
Identification of Ligands for Ion Channels: TRPM2
Gu, Y., Liu, M., Ma, L., & Quinn, R. J. (2024) · ChemBioChem · doi:10.1002/cbic.202300790 · Cited by 7 · see citing works
de Oliveira, P. C. O., do Amaral, B. S., Cardoso, C. L., Cass, Q. B., & de Moraes, M. C. (2025) · Journal of Pharmaceutical Analysis · doi:10.1016/j.jpha.2025.101379 · Cited by 7 · see citing works
Gu, Y., Liu, M., Ma, L., & Quinn, R. J. (2024) · ACS Chemical Biology · doi:10.1021/acschembio.3c00781 · Cited by 6 · see citing works
Affinity selection mass spectrometry (AS-MS) for prospecting ligands in natural product libraries
do Amaral, B. S., de Moraes, M. C., Cardoso, C. L., & Cass, Q. B. (2025) · Frontiers in Natural Products · doi:10.3389/fntpr.2025.1562501 · Cited by 6 · see citing works
James, W. L. (2000) · Encyclopedia of Analytical Chemistry · doi:10.1002/9780470027318.a1402 · Cited by 6 · see citing works
Gopinath, S. C. B. (2011) · Encyclopedia of Analytical Chemistry · doi:10.1002/9780470027318.a1402.pub2 · Cited by 6 · see citing works
Medical applications of aptamers
Vallian, S., & Khazaei, M. (2009) · Research in Pharmaceutical Sciences · Cited by 5 · see citing works
Emerging challenges in ligand discovery: new opportunities for chromatographic assay
Ng, E. S. M., & Schriemer, D. C. (2005) · Expert Review of Proteomics · doi:10.1586/14789450.2.6.891 · Cited by 5 · see citing works
pyBinder: Quantitation to Advance Affinity Selection-Mass Spectrometry
Brown, J. S., Lee, M. A., Vuong, W., Loas, A., & Pentelute, B. L. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.4c04445 · Cited by 5 · see citing works
Huang, T., Chai, X., Li, S., Liu, B., Zhan, J., Wang, X., Xiao, X., Zhu, Q., Liu, C., Zeng, D., et al. (2024) · Analytical Chemistry · doi:10.1021/acs.analchem.4c02255 · Cited by 4 · see citing works
Studying Noncovalent Small Molecule Interactions with Protein and RNA Targets by Mass Spectrometry
Loo, J. A., Thanabal, V., & Mei, H. (2000) · Humana Press eBooks · doi:10.1007/978-1-59259-719-2_5 · Cited by 4 · see citing works
Mass Spectrometry and Drug Discovery
van Breemen, R. B., Newsome, A. G., & Dahl, J. H. (2010) · Burger's Medicinal Chemistry and Drug Discovery · doi:10.1002/0471266949.bmc012.pub2 · Cited by 4 · see citing works
Guild, B. C. (1999) · Annual reports in medicinal chemistry · doi:10.1016/s0065-7743(08)60585-0 · Cited by 3 · see citing works
Nemeth, G. A., & Kassel, D. B. (2001) · Annual reports in medicinal chemistry · doi:10.1016/s0065-7743(01)36067-0 · Cited by 3 · see citing works
Gopinath, S. C. B. (2016) · Encyclopedia of Analytical Chemistry · doi:10.1002/9780470027318.a1402.pub3 · Cited by 3 · see citing works
Mass Spectrometry and Drug Discovery
van Breemen, R. B. (2003) · Burger's Medicinal Chemistry and Drug Discovery · doi:10.1002/0471266949.bmc012 · Cited by 3 · see citing works
Affinity selection mass spectrometry (AS-MS) as a tool to accelerate drug discovery efforts
Pandey, S., Samain, F., Nacham, O., Williams, J. D., & Elsen, N. L. (2026) · Expert Opinion on Drug Discovery · doi:10.1080/17460441.2026.2622373 · Cited by 2 · see citing works
Drug Design Methods to Regulate Protein–Protein Interactions
Poluri, K. M., Gulati, K., Tripathi, D. K., & Nagar, N. (2023) · Springer eBooks · doi:10.1007/978-981-99-2423-3_6 · Cited by 2 · see citing works
Vouros, P., & Fang, A. S. (2000) · CRC Press eBooks · doi:10.1201/9780203909966.ch2 · Cited by 1 · see citing works
Miller, S. M., Moos, W. H., Munk, B. H., & Munk, S. A. (2016) · Elsevier eBooks · doi:10.1016/b978-0-08-100625-2.00011-8 · Cited by 1 · see citing works
Kool, J., Vermeulen, N. P. E., Lingeman, H., Derks, R. J. E., & Irth, H. (2008) · doi:10.1002/9783527623259.ch17 · Cited by 1 · see citing works
Lee, M. S. (2002) · doi:10.1002/0471218812.refs · Cited by 1 · see citing works
Mass Spectrometry and Drug Discovery
van Breemen, R. B., Newsome, A. G., Dahl, J. H., & Muchiri, R. N. (2021) · Burger's Medicinal Chemistry and Drug Discovery · doi:10.1002/0471266949.bmc012.pub3 · Cited by 1 · see citing works
pyBinder: Label-free Quantitation to Advance Affinity Selection-Mass SpectrometryPreprint
Brown, J. S., Lee, M. A., Vuong, W., Loas, A., & Pentelute, B. L. (2024) · ChemRxiv · doi:10.26434/chemrxiv-2024-lfqjb · Cited by 1 · see citing works
Combinatorial Chemistry Libraries, Analysis of
Kibbey, C. E. (2000) · Encyclopedia of Analytical Chemistry · doi:10.1002/9780470027318.a1907
Nanotechnology: A novel approach towards cancer treatment
Goyal, R., & Kumar, S. (2014)
The U se of 19F NMR to Monitor Organic Reactions on Solid Supports
Preserve, L. (2004)
Black, T. A. (2003) · CRC Press eBooks · doi:10.1201/9780203911464.ch13
Kyranos, J. N., & Chipman, S. D. (2000) · CRC Press eBooks · doi:10.1201/9780203909966.ch8
Mechanism of Action and Metabolism of Antineoplastic and Chemopreventive Agents
(2002) · CRC Press eBooks · doi:10.1201/9781420042696.ch5
English, R. D., & Haag, A. M. (2011) · Pharmaceutical Sciences Encyclopedia · doi:10.1002/9780470571224.pse427
Avital‐Shmilovici, M., Liu, X., Shaler, T. A., Lowenthal, A. C., Bourbon, P., Snider, J., Tambo-ong, A., Repellin, C. E., Yniguez, K., Sambucetti, L. C., et al. (2021) · doi:10.33774/chemrxiv-2021-6wxh5-v3
Wang, M., Fu, S., Xu, Y., Gao, J., Ren, J., Yang, X., Xu, H., Sun, G., Li, Y., Wang, Y., et al. (2026) · Advanced Science · doi:10.1002/advs.77386
BindingDB Entry 50036753: Mixture-based synthetic combinatorial libraries.
BindingDB (2012) · UC San Diego · doi:10.7270/q22z167w
BindingDB Entry 1965: Affinity-based ranking of ligands for DPP-4 from mixtures
BindingDB (2007) · UC San Diego · doi:10.7270/q2m906wf
- Authors
- Kaur, S., McGuire, L., Tang, D., Dollinger, G., & Huebner, V.
- Affiliations
- Biochimie des Protéines Membranaires (FR)
- Venue
- Journal of Protein Chemistry
- Published
- 1997-07
- Volume
- 16
- Issue
- 5
- Pages
- 505-511
- ISSN
- 0277-8033 (ISSN-L)
- 1573-4943
- OpenAlex
- W1226074361
- Cited by
- 95
- References
- 15
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 5 · 2023: 4 · 2022: 5 · 2021: 6 · 2020: 2 · 2017: 1 · 2016: 2 · 2014: 1 · 2013: 2
- FWCI
- 5.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Protein Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chemical Synthesis and Analysis
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- affinity selection, combinatorial libraries, lead compound identification, small molecule library, protein-ligand interactions, receptor binding, drug discovery, library optimization
- MeSH terms
- Binding, Competitive, Chromatography, Affinity, Drug Evaluation, Preclinical, Ligands, Proteins, Receptors, Drug, Mass Spectrometry, Structure-Activity Relationship, Drug Design, Peptide Library
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
