CoChat
Sign Up
Cited by 147Microtubule and mitosis dynamicsCellular transport and secretionNuclear Structure and Function

Cargo binding and regulatory sites in the tail of fungal conventional kinesin

Seiler, Stephan, Kirchner, Jochen, Horn, Christian, Kallipolitou, Athina, Woehlke, Günther, Schliwa, Manfred·2000·Nature Cell Biology
DOI 10.1038/35014022
Open this paper in CoChat — free account

Abstract

Here, using a quantitative in vivo assay, we map three regions in the carboxy terminus of conventional kinesin that are involved in cargo association, folding and regulation, respectively. Using C-terminal and internal deletions, point mutations, localization studies, and an engineered 'minimal' kinesin, we identify five heptads of a coiled-coil domain in the kinesin tail that are necessary and sufficient for cargo association. Mutational analysis and in vitro ATPase assays highlight a conserved motif in the globular tail that is involved in regulation of the motor domain; a region preceding this motif participates in folding. Although these sites are spatially and functionally distinct, they probably cooperate during activation of the motor for cargo transport.

Integrity signals

Registry

Crossref · 10.1038/35014022

Cites

51 of 51 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The truncated KHC protein (KHC-891) was largely found at the tips of cellular processes.

    “Indeed, the truncated KHC protein was largely found at the tips of cellular processes (Fig. 5 D), consistent with its uninhibited migration towards the plus end of MTs and in agreement with similar results in COS cells (Verhey et al. 1998) and Neurospora (Seiler et al. 2000).”

    From Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules (2001) · cites this paper as Seiler et al. 2000

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Seiler, S., Kirchner, J., Horn, C., Kallipolitou, A., Woehlke, G., & Schliwa, M.
Affiliations
Adolf-Butenandt-Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie (DE)
Corresponding author
Schliwa, Manfred
ORCID
Woehlke, Günther
Venue
Nature Cell Biology
Publisher
Springer Science and Business Media LLC
Published
2000-05-09
Volume
2
Issue
6
Pages
333-338
ISSN
  • 1465-7392 (ISSN-L)
  • 1476-4679
DOI
10.1038/35014022
OpenAlex
W1481640083
Semantic Scholar
67afb86f9a3c9a012f0e51d501a82feb20c97fbc
Cited by
147
References
50
Citations by year
2025: 2 · 2024: 1 · 2023: 7 · 2022: 8 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 5 · 2016: 3 · 2015: 1 · 2014: 4 · 2013: 5 · 2012: 7
FWCI
4.28
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature Cell Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Deutsche Forschungsgemeinschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
cargo binding, coiled-coil domain, heptad repeat, protein folding, ATPase activity, freight transport, motor activation
MeSH terms
Adenosine Triphosphatases, Amino Acid Sequence, Binding Sites, Fluorescent Antibody Technique, Fungal Proteins, Genetic Complementation Test, Kinetics, Molecular Sequence Data, Mutation, Neurospora, Phenotype, Recombinant Fusion Proteins, Substrate Specificity, Kinesins, Conserved Sequence, Protein Structure, Tertiary, Protein Folding, Amino Acid Substitution, Molecular Motor Proteins, Amino Acid Motifs

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review