A transcription reinitiation intermediate that is stabilized by activator
Abstract
High levels of gene transcription by RNA polymerase II depend on high rates of transcription initiation and reinitiation. Initiation requires recruitment of the complete transcription machinery to a promoter, a process facilitated by activators and chromatin remodelling factors. Reinitiation probably occurs through a different pathway. After initiation, a subset of the transcription machinery remains at the promoter, forming a platform for assembly of a second transcription complex. Here we describe the isolation of a reinitiation intermediate that includes transcription factors TFIID, TFIIA, TFIIH, TFIIE and Mediator. This intermediate can act as a scaffold for formation of a functional reinitiation complex. Formation of this scaffold is dependent on ATP and TFIIH. The scaffold is stabilized in the presence of the activator Gal4-VP16, but not Gal4-AH, suggesting a new role for some activators and Mediator in promoting high levels of transcription.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Core transcription complexes enforce a high transcription rate in an ATP-dependent fashion by promoting re-initiation of the transcription cycle.
“The core transcription complexes of which these factors are members have been demonstrated to enforce a high transcription rate in an ATP-dependent fashion by promoting re-initiation of the transcription cycle47.”
From Neuron Enriched Nuclear Proteome Isolated from Human Brain (2013) · cites this paper as [47]
Cited by
Sorted by citations
Single-RNA counting reveals alternative modes of gene expression in yeast
Zenklusen, D., Larson, D. R., & Singer, R. H. (2008) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1514 · Cited by 684 · see citing works
Defining mechanisms that regulate RNA polymerase II transcription in vivo
Fuda, N. J., Ardehali, M. B., & Lis, J. T. (2009) · Nature · doi:10.1038/nature08449 · Cited by 576 · see citing works
Genome-wide structure and organization of eukaryotic pre-initiation complexes
Rhee, H. S., & Pugh, B. F. (2012) · Nature · doi:10.1038/nature10799 · Cited by 496 · see citing works
The Mediator complex and transcription regulation
Poss, Z. C., Ebmeier, C. C., & Taatjes, D. J. (2013) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409238.2013.840259 · Cited by 364 · see citing works
Genome-wide Single-Molecule Footprinting Reveals High RNA Polymerase II Turnover at Paused Promoters
Krebs, A. R., Imanci, D., Hoerner, L., Gaidatzis, D., Burger, L., & Schübeler, D. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.06.027 · Cited by 249 · see citing works
Transcription in Living Cells: Molecular Mechanisms of Bursting
Rodriguez, J., & Larson, D. R. (2020) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-011520-105250 · Cited by 224 · see citing works
van de Peppel, J., Kettelarij, N., van Bakel, H., Kockelkorn, T. T. J. P., van Leenen, D., & Holstege, F. C. P. (2005) · Molecular Cell · doi:10.1016/j.molcel.2005.06.033 · Cited by 211 · see citing works
Adkins, M. W., & Tyler, J. K. (2006) · Molecular Cell · doi:10.1016/j.molcel.2005.12.010 · Cited by 162 · see citing works
Chapter 4 β‐Globin Regulation and Long‐Range Interactions
Palstra, R., de Laat, W., & Grosveld, F. (2008) · Advances in genetics · doi:10.1016/s0065-2660(07)00004-1 · Cited by 131 · see citing works
Regulation of the p53 transcriptional response by structurally diverse core promoters
Morachis, J. M., Murawsky, C. M., & Emerson, B. M. (2010) · Genes & Development · doi:10.1101/gad.1856710 · Cited by 86 · see citing works
Herpes Simplex Virus 1 ICP4 Forms Complexes with TFIID and Mediator in Virus-Infected Cells
Lester, J. T., & DeLuca, N. A. (2011) · Journal of Virology · doi:10.1128/jvi.00385-11 · Cited by 74 · see citing works
Neuron Enriched Nuclear Proteome Isolated from Human Brain
Dammer, E. B., Duong, D. M., Diner, I., Gearing, M., Feng, Y., Lah, J. J., Levey, A. I., & Seyfried, N. T. (2013) · Journal of Proteome Research · doi:10.1021/pr400246t · Cited by 70 · see citing works
- Authors
- Yudkovsky, N., Ranish, J. A., & Hahn, S.
- Affiliations
- University of Washington (US)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Hahn, Steven
- Venue
- Nature
- Published
- 2000-11
- Volume
- 408
- Issue
- 6809
- Pages
- 225-229
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1601315441
- Cited by
- 421
- References
- 0
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 12 · 2023: 6 · 2022: 9 · 2021: 10 · 2020: 8 · 2019: 13 · 2018: 12 · 2017: 10 · 2016: 13 · 2015: 7 · 2014: 16 · 2013: 25 · 2012: 24
- FWCI
- 9.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- Damon Runyon Cancer Research Foundation
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- transcription reinitiation, RNA polymerase II, transcription initiation, TFIIH, Mediator complex, TFIID, TFIIA, TFIIE, transcriptional activator, GAL4-VP16
- MeSH terms
- Adenosine Triphosphate, DNA-Binding Proteins, DNA, Fungal, Fungal Proteins, Promoter Regions, Genetic, RNA Polymerase II, RNA, Fungal, Transcription Factors, Transcription, Genetic, Yeasts, Trans-Activators, Transcription Factors, TFII, Saccharomyces cerevisiae Proteins, Transcription Factor TFIID, TATA-Binding Protein Associated Factors, Transcription Factor TFIIA, Macromolecular Substances, Transcription Factor TFIIH
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
